C1q gene and application of coding protein of C1q gene

Abstract

The invention provides a C1q gene and an application of a coding protein of the C1q gene. The C1q gene and the application of the coding protein of the C1q gene are used for preparing products distinguishing tuberculosis latent infection and active tuberculosis. The products distinguishing tuberculosis latent infection and active tuberculosis preferably comprise products using real-time quantitative polymerase chain reaction (PCR) and gene chip detection or immune detection to distinguish the tuberculosis latent infection and the active tuberculosis. Experiments prove that expression of the C1q gene in blood of tuberculosis patients is evidently stronger than expression of the C1q gene in blood of health people and latent infection people, and thus the C1q gene can act as a distinctive marker gene in diagnosing the tuberculosis, and tuberculosis diagnosis is accurate and fast.

Description

Technical field: [0001] The invention relates to the technical field of bioengineering, in particular to the application of C1q gene and its coded protein. Background technique: [0002] Tuberculosis (TB) is a chronic infectious disease caused by Mycobacterium tuberculosis infection. Mycobacterium tuberculosis can not only cause pulmonary tuberculosis (85%), but also cause tuberculosis in multiple organs outside the lungs. Although there are currently effective anti-tuberculosis drugs, tuberculosis is still the number one killer of infectious diseases, and about 2 million people die from tuberculosis every year in the world; about one-third of the world's population is infected with Mycobacterium tuberculosis, which is called About 10% of people with latent tuberculosis infection (LTBI) will eventually develop active tuberculosis. [0003] Due to the lack of effective tuberculosis vaccine, the prevention and control of tuberculosis mainly depends on early detection, treatme...

AI Technical Summary

Problems solved by technology

However, current detection techniques for diagnosing active tuberculosis have serious deficiencies and cannot meet the requirements of clinical and tuberculosis prevention and control: 1) Sputum Mycobacterium tuberculosis microbiological examination is highly specific and is currently the gold standard for diagnosing active tuberculosis. Low reliability (less than 40%), long time-consuming (1-2 months for tuberculosis culture), and high requirements for laboratory biosafety

2) Mycobacterium tuberculosis gene detection, although the purpose of rapid diagnosis (1 day) has been achieved, the sensitivity of genetic detection directly from sputum samples has not been significantly improved, and there are problems of false negatives and false positives

3) Antibody detection in immunological testing is considered unsuitable for the diagnosis of tuberculosis by the World Health Organization; cellular immunological testing, including tuberculin skin test (TST) and tuberculosis interferon release test (IGRA), cannot effectively distinguish patients with active tuberculosis and latent tuberculosis infection, although the latter is significantly more sensitive than other tests in patients with active tuberculosis

[0005] There is no report about C1q gene as a diagnostic marker for tuberculosis

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