Preparation method and application of multi-effect compound anti-biological inoculants
A biocontrol agent and strain technology, which is applied in the field of preparation of multi-effect composite biocontrol agents, can solve problems such as unstable biological control of Trichoderma, and achieve the effect of maintaining natural balance.
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Embodiment 1
[0033] A: Preparation of slant mother strains:
[0034] Take 10 grams of crop root surface soil, add sterile water to dilute to 1000 ml of dilution, mix the dilution with PDA medium in a weight ratio of 1:9, pour it into a petri dish to make a plate, and cultivate it at 26°C for 48 hours , Trichoderma was obtained by isolation; after purification, it was transferred to the slope of the test tube as the mother strain of the slope;
[0035] PDA medium: 200 grams of potato, 20 grams of glucose, 20 grams of agar, 0.05 grams of Bengal red, 0.03 grams of streptomycin sulfate, 1000 ml of water;
[0036] Take 10 grams of crop roots, sterilize the surface with 0.1% mercuric chloride solution for 2 minutes, then wash with sterile water for 3 times, grind it, add sterile water to dilute to 1000 ml dilution, and mix the dilution with PDA medium: press After mixing in a weight ratio of 1:9, pour it into a petri dish to make a plate, culture it at 26°C for 48 hours, and obtain endophytic B...
Embodiment 2
[0046] A: Preparation of slant mother strains:
[0047] Take 10 grams of crop root surface soil, add sterile water to dilute to 1000 ml of dilution, mix the dilution with PDA medium in a weight ratio of 1:9, pour it into a petri dish to make a plate, and cultivate it at 26°C for 48 hours , Trichoderma was obtained by isolation; after purification, it was transferred to the slant of the test tube as the mother strain of the slant.
[0048] PDA medium: 200 grams of potato, 20 grams of glucose, 20 grams of agar, 0.05 grams of Bengal red, 0.03 grams of streptomycin sulfate, 1000 ml of water;
[0049] Take 10 grams of crop root system, sterilize the surface with 0.1% mercuric chloride solution for 3 minutes, then wash it with sterile water for 4 times, grind it, add sterile water to dilute to 1000 ml dilution, and dilute the dilution with PDA medium by 1 : 9 after being mixed in a weight ratio and poured into a petri dish to make a flat plate, cultured at 26°C for 48 hours, and th...
Embodiment 3
[0060] A: Preparation of slant mother strains:
[0061] Take 10 grams of crop root surface soil, add sterile water to dilute to 1000 ml of dilution, mix the dilution with PDA medium in a weight ratio of 1:9, pour it into a petri dish to make a plate, and cultivate it at 26°C for 48 hours , Trichoderma was obtained by isolation; after purification, it was transferred to the slope of the test tube as the mother strain of the slope;
[0062] PDA medium: 200 grams of potato, 20 grams of glucose, 20 grams of agar, 0.05 grams of Bengal red, 0.03 grams of streptomycin sulfate, 1000 ml of water;
[0063] Take 10 grams of crop root system, sterilize the surface with 0.1% mercuric chloride solution for 4 minutes, then wash it with sterile water for 5 times, grind it, add sterile water to dilute to 1000 ml dilution, and dilute the dilution with PDA medium by 1 : After mixing in a weight ratio of 9, pour it into a petri dish to make a plate, cultivate it for 48 hours at 26° C., and obtai...
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