Preparation method and application of multi-effect compound anti-biological inoculants

A biocontrol agent and strain technology, which is applied in the field of preparation of multi-effect composite biocontrol agents, can solve problems such as unstable biological control of Trichoderma, and achieve the effect of maintaining natural balance.

Inactive Publication Date: 2013-06-12
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Utilizing the different modes of action and biocontrol mechanisms of biocontrol Trichoderma and endogenous Bacillus subtilis to control crop soil-borne diseases, the synergistic application of the two will become an important means to improve the control effect of crop soil-borne diseases, and can solve the biological control effect of Trichoderma Insufficient instability

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A: Preparation of slant mother strains:

[0034] Take 10 grams of crop root surface soil, add sterile water to dilute to 1000 ml of dilution, mix the dilution with PDA medium in a weight ratio of 1:9, pour it into a petri dish to make a plate, and cultivate it at 26°C for 48 hours , Trichoderma was obtained by isolation; after purification, it was transferred to the slope of the test tube as the mother strain of the slope;

[0035] PDA medium: 200 grams of potato, 20 grams of glucose, 20 grams of agar, 0.05 grams of Bengal red, 0.03 grams of streptomycin sulfate, 1000 ml of water;

[0036] Take 10 grams of crop roots, sterilize the surface with 0.1% mercuric chloride solution for 2 minutes, then wash with sterile water for 3 times, grind it, add sterile water to dilute to 1000 ml dilution, and mix the dilution with PDA medium: press After mixing in a weight ratio of 1:9, pour it into a petri dish to make a plate, culture it at 26°C for 48 hours, and obtain endophytic B...

Embodiment 2

[0046] A: Preparation of slant mother strains:

[0047] Take 10 grams of crop root surface soil, add sterile water to dilute to 1000 ml of dilution, mix the dilution with PDA medium in a weight ratio of 1:9, pour it into a petri dish to make a plate, and cultivate it at 26°C for 48 hours , Trichoderma was obtained by isolation; after purification, it was transferred to the slant of the test tube as the mother strain of the slant.

[0048] PDA medium: 200 grams of potato, 20 grams of glucose, 20 grams of agar, 0.05 grams of Bengal red, 0.03 grams of streptomycin sulfate, 1000 ml of water;

[0049] Take 10 grams of crop root system, sterilize the surface with 0.1% mercuric chloride solution for 3 minutes, then wash it with sterile water for 4 times, grind it, add sterile water to dilute to 1000 ml dilution, and dilute the dilution with PDA medium by 1 : 9 after being mixed in a weight ratio and poured into a petri dish to make a flat plate, cultured at 26°C for 48 hours, and th...

Embodiment 3

[0060] A: Preparation of slant mother strains:

[0061] Take 10 grams of crop root surface soil, add sterile water to dilute to 1000 ml of dilution, mix the dilution with PDA medium in a weight ratio of 1:9, pour it into a petri dish to make a plate, and cultivate it at 26°C for 48 hours , Trichoderma was obtained by isolation; after purification, it was transferred to the slope of the test tube as the mother strain of the slope;

[0062] PDA medium: 200 grams of potato, 20 grams of glucose, 20 grams of agar, 0.05 grams of Bengal red, 0.03 grams of streptomycin sulfate, 1000 ml of water;

[0063] Take 10 grams of crop root system, sterilize the surface with 0.1% mercuric chloride solution for 4 minutes, then wash it with sterile water for 5 times, grind it, add sterile water to dilute to 1000 ml dilution, and dilute the dilution with PDA medium by 1 : After mixing in a weight ratio of 9, pour it into a petri dish to make a plate, cultivate it for 48 hours at 26° C., and obtai...

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PUM

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Abstract

The invention provides a preparation method and an application of multi-effect anti-biological inoculants for controlling crop soil-borne diseases. According to the preparation method, trichoderma viride strains and endogeny bacillus subtilis having antagonistic action to pathogenic bacteria of the crop soil-borne diseases are used as parent inoculants, and light calcium carbonate is used as a carrier to prepare the anti-biological inoculants. The anti-biological inoculants are mainly used for controlling various crop soil-borne diseases caused by pathogenic fungi including fusarium, pythium, rhizoctonia and the like, and have multiple functions of improving self resistance and increasing the output of plants. By adopting the preparation method, the anti-biological inoculants are short in production cycle, stable and lasting in control effect and pollution-free to the environment without residues to crops.

Description

technical field [0001] The invention relates to a biocontrol agent, in particular to a preparation method and application of a multi-effect compound biocontrol agent for preventing and treating soil-borne diseases of crops. The two bacterial strains are respectively Trichoderma (Trichoderma viride ) and Bacillus subtilis ( Bacillus subtilis ). Background technique [0002] As more and more toxic chemical pesticides are restricted in use, countries around the world are stepping up research and development of biological pesticides. As a class of antagonistic fungi with important biocontrol value, Trichoderma has been found to have antagonistic effects on plant pathogenic fungi as early as the 1930s, and its mechanism of action includes antibiotic production, parasitism, bacteriolysis, and competition. The strong competition and viability of Trichoderma play an important role in the process of inhibiting pathogenic bacteria, especially soil-borne diseases, and it is a biocon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N63/00A01P3/00C12N3/00C12R1/125C12R1/885
Inventor 郑雅楠杨长成魏汉莲高增贵李可强关昕庄敬华
Owner SHENYANG AGRI UNIV
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