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Application of bacteroides fragilis in preparing composition for preventing and treating hypercholesteremia

A technology for hypercholesterolemia and Bacteroides fragilis, which is applied in application, food preparation, drug combination and other directions to achieve the effects of good preventive and therapeutic effects, strong pharmacological effects, good food and medicinal prospects

Inactive Publication Date: 2013-06-12
广州知光生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as a probiotic, Bacteroides fragilis has not been reported yet for its application in the preparation of compositions for preventing and treating hypercholesterolemia.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Preparation of Bacteroides fragilis bacterial liquid

[0019] (1) Isolation of Bacteroides fragilis from infant feces

[0020] Weigh 0.5 g of stool according to the "normal flora test method", put it in a test tube filled with 4.5 ml of normal saline, and serially dilute it to 10 -1 ~10 -6 , from high concentration to low concentration on the Bd medium plate, placed in an anaerobic gas box and anaerobic gas bag for anaerobic culture. Store at 37°C, open the can for inspection after 2-7 days. A single colony was selected for pure culture, staining microscopy, culture characteristics, and physiological and biochemical testing.

[0021] (2) Enrichment culture of Bacteroides fragilis and preparation of bacterial solution

[0022] Select the purified single colony and inoculate it in the improved tryptone broth for enrichment culture. The obtained bacterial solution is centrifuged and precipitated at a speed of 3000r / min for 15 minutes, and the supernatant is...

Embodiment 2

[0023] Example 2 Toxicity test of Bacteroides fragilis

[0024] The Bacteroides fragilis bacterium liquid that adopts embodiment 1 preparation carries out following experiment:

[0025] (1) Acute toxicity test in mice

[0026] Sixty C57BR / C mice weighing 22±2 g (half male and half male) were divided into high-dose group, low-dose group and control group, 20 in each group. Each mouse in the high-dose group was intragastrically administered 10 12 cfu / ml bacterial solution 0.5ml, the low dose group was administered orally 10 times a day 9 cfu / ml bacterial solution 0.5ml, and the control group was fed with 0.5ml normal saline every day. Continuous observation for 14 days.

[0027] Experimental results: The mice grew normally, no death was observed, and the LD50 was undetectable.

[0028] (2) Rat toxicity test

[0029] Take 30 SD rats (half male and half female) of 150±20 g, and divide them into 3 groups: high and low dose group and control group. Each rat in the high-do...

Embodiment 3

[0034] Example 3 Prevention and treatment effect of Bacteroides fragilis on hypercholesterolemia

[0035] The Bacteroides fragilis bacterium liquid that adopts embodiment 1 preparation carries out following experiment:

[0036] SD rats with a body weight of 200±20 grams were taken. After one week of adaptation to the environment, blood was taken from the tail vein to measure blood lipid levels. Rats with abnormal blood lipids were excluded. The SD rats were randomly divided into 3 groups: normal group, high-fat group, and treatment group, with 10 rats in each group. The normal group was given normal feed (protein 14%, fat 10%, carbohydrate 76%); the high-fat group and the treatment group were given high-fat feed (adding 2% cholesterol, 1% cholic acid, 5% peanut oil); the treatment group Gavage 2ml 10 per day 9 cfu / ml of Bacteroides fragilis suspension.

[0037] After 8 weeks, they fasted overnight and took blood from the heart after ether anesthesia to measure serum total...

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Abstract

The invention relates to the technical field of application of bacteroides fragilis, and in particular relates to application of bacteroides fragilis in preparing a composition for preventing and treating hypercholesteremia. Experiments show that bacteroides fragilis is safe, nontoxic and strong in pharmacologic action and has good preventing and treating effect to hypercholesteremia, thereby indicating that the bacteroides fragilis has good edible and officinal prospects. The invention explores novel use of bacteroides fragilis and develops a novel application field. Bacteroides fragilis as probiotic can be used for preparing foods or medical compositions for preventing and treating hypercholesteremia so as to provide a clinically health-care and preventing and treating food suitable for human to take.

Description

technical field [0001] The invention relates to the technical field of application of Bacteroides fragilis, in particular to the application of Bacteroides fragilis in preparing a composition for preventing and treating hypercholesterolemia. Background technique [0002] Hypercholesterolemia is one of the main risk factors for atherosclerosis and related cardiovascular diseases (such as coronary heart disease). How to reduce serum cholesterol has become a topic of increasing concern to modern nutritionists and clinicians. Hypercholesterolemia has no obvious clinical symptoms and is often found during physical examination. The current prevention and control measures mainly include behavioral prevention and drug treatment. In recent decades, it has been found that people who drink yogurt for a long time have a significantly lower risk of cardiovascular disease, and a large number of scientific studies have confirmed that this is related to the probiotics contained in yogurt....

Claims

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Application Information

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IPC IPC(8): A61K35/74A61P3/06A23L1/30A23C9/152A23C9/13A23C19/00A23G9/36
Inventor 智发朝白杨刘洋洋
Owner 广州知光生物科技有限公司
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