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Paenibacillus terrae strain

A bacillus and land technology, applied in the directions of bacteria, microorganisms, biochemical equipment and methods, etc., can solve the problems of waste, easy loss, environmental pollution, etc., and achieve the effect of strong tolerance and promotion of plant growth.

Active Publication Date: 2013-06-12
黑龙江省农垦科学院农畜产品综合利用研究所 +1
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] Nitrogen, phosphorus, and potassium are macronutrient elements necessary for plant growth. The total phosphorus content in the soil cultivation layer is 0.1-2.0g / kg, of which inorganic phosphorus accounts for 15%-80% of the total phosphorus, most of which are insoluble The inorganic phosphorus compounds are difficult to be directly used by plants; the total potassium content in the soil cultivation layer is 0.5-45g / kg, of which mineral potassium accounts for 90%-98% of the total potassium, which is difficult to be directly used by plants; agricultural production The utilization rate of the applied phosphorus fertilizer and potassium fertilizer is only 40% to 60%, and the rest is refixed by the soil. Due to the low utilization rate of crops, it is easy to lose, causing serious waste and environmental pollution.

Method used

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Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0022] Specific Embodiment 1: In this embodiment, Paenibacillus terrae NK3-4 is preserved in the General Microorganism Center of China Committee for the Collection of Microorganisms, and the preservation number is CGMCC No.7011.

[0023] The sampling location of Paenibacillus terrae NK3-4 in this embodiment is the experimental field (46°46′02.8″N; 130°25′44.5″E) in the Heilongjiang Academy of Agricultural Sciences, Jiamusi City, Heilongjiang Province, China.

[0024] Collection, isolation and screening of Paenibacillus terrae NK3-4 strain:

[0025] In July 2005, in a soybean field that has been replanted for 4 years, select healthy soybean plants, uproot them, collect the soil around the roots, collect samples from the sampling site, classify and number each sample, and put them into sterilized plants. Put them in a sealed polyethylene bag, put them in an ice box and bring them back to the laboratory, store them at 4°C, and carry out enrichment culture within 2 days. Then put...

Embodiment 1

[0035] Pick Paenibacillus terrae (Paenibacillus terrae) NK3-4 bacterial lawn and inoculate it in potassium feldspar liquid medium (potassium feldspar liquid medium consists of 10.0g sucrose, 0.2g K 2 HPO 4 , 0.2g MgSO 4 ·7H 2 O, 0.2g NaCl, 5.0g CaCO 3 , 1.0g potassium feldspar powder, 0.1g CaSO 4 , 2.0g yeast powder and 1L distilled water, pH value 7.2), in addition, Paenibacillus terrae (Paenibacillus terrae) NK3-4 was inoculated in the control medium without potassium feldspar powder (the control medium consisted of 10g sucrose, 0.2 k 2 HPO 4 , 0.2g MgSO 4 ·7H 2 O, 0.2g NaCl, 5.0g CaCO 3 , 0.1g CaSO 4 , 2.0g yeast powder and 1L distilled water, the pH value is 7.2), the potassium feldspar liquid medium without inoculation was used as the blank control, the experiment was set in 4 replicates, and cultured at 30°C and 200r / min for 24h, spare.

[0036] Take the bacterial solution cultured for 24 hours and dilute it 10 times, measure the concentration of potassium in ...

Embodiment 2

[0041] Put the selected soybean seeds into 0.1% sodium hypochlorite solution for disinfection for 3 minutes, then take them out and rinse them with sterile water quickly, and then transfer them to a sterile plate with a sterilized round filter paper, 15 grains / plate, add Paenibacillus terrae (Paenibacillus terrae) NK3-4 liquid biological preparation (hereinafter collectively referred to as NK3-4 liquid biological preparation) 10ml, and adding 10ml sterile water as the control group, repeated 9 times, and measured the germination potential on the third day, The germination rate was measured on the 6th day.

[0042] The experimental results are shown in Table 2. The NK3-4 liquid biological preparation can increase the germination rate of soybean seeds by 4.4 percentage points.

[0043] Experimental conclusion: NK3-4 liquid biological preparation can be used for pre-sowing treatment of soybean seeds.

[0044] Table 2

[0045]

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Abstract

The invention relates to paenibacillus elgii, particularly a Paenibacillus terrae strain. The Paenibacillus terrae NK3-4 is collected at China General Microbiological Culture Collection Center, and the collection number is CGMCC No.7011. The NK3-4 has the functions of fixing nitrogen in air, decomposing mineral phosphorus, mineral potassium and insoluble silicon, and inhibiting phytopathogens. The Paenibacillus terrae NK3-4 can still increase the yield of rice, corn, soybean and other crops under the conditions of reducing the application amount of the fertilizer, and especially has more obvious yield increase amplitude when being matched with a silicon fertilizer. The invention is applicable to the field of agriculture.

Description

technical field [0001] The present invention relates to a Paenibacillus. Background technique [0002] The genus Paenibacillus (Paenibacillus Ash, Priest & Collin, 1994) belongs to the phylum Firmicutes, the class Bacillus, the order Bacillus, and the family Paenibacillus. Cells are rod-shaped, Gram-positive, negative, or variable, and move with peripatetic flagella. There are oval spores in enlarged cysts, and there is no soluble pigment on nutrient agar. Facultatively anaerobic or strictly aerobic. At least 39 species have been recorded, and Paenibacillus is widely distributed in nature. [0003] The research on the known Paenibacillus found that it has extremely high application value. For example, Paenibacillus polymyxa (P.polymyxa) can prevent and control pathogenic bacteria, pathogenic fungi, root-knot nematodes and other harmful microorganisms, and can not only secrete a variety of peptide antibacterial substances (such as polymyxin, bacteriocin, cholipeptide, po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 于文清刘文志李鹏胡广民隋文志赵晓锋田艳洪
Owner 黑龙江省农垦科学院农畜产品综合利用研究所
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