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Application of Safenour cyclopeptide in oral insulin medicine for treating diabetes

A diabetes and insulin technology, applied in the direction of cyclic peptide components, drug combinations, medical preparations containing active ingredients, etc., can solve the problems that insulin is easily degraded by enzymes, cannot be taken orally, and is inconvenient to carry, and achieves significant resistance to protease degradation and effective Effects of lowering blood sugar and promoting intestinal absorption

Inactive Publication Date: 2014-06-04
武汉光谷世傲生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention solves the problems that insulin is easily degraded by enzymes, cannot be taken orally and is not easy to carry

Method used

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  • Application of Safenour cyclopeptide in oral insulin medicine for treating diabetes
  • Application of Safenour cyclopeptide in oral insulin medicine for treating diabetes
  • Application of Safenour cyclopeptide in oral insulin medicine for treating diabetes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] A preparation method of Bacillus amyloliquefaciens WH3 bacterial strain, the steps are:

[0057] 1. Screening and isolation of Bacillus amyloliquefaciens WH3:

[0058] Rapeseed seedlings at the three-leaf stage were randomly selected from the rapeseed field of Hubei Academy of Agricultural Sciences in Wuhan City, Hubei Province, China, and the roots were separated after washing. Rinse the outer surface of the root of rapeseed with tap water, rinse it with sterile water for 5 times, then immerse it in 70% (V / V) ethanol for 30 seconds, take it out, rinse it with sterile water for 5 times, and cut it with sterile scissors. The rice roots were cut into pieces about 1 mm in size, and then placed on PDA medium (containing 200 g of potato boiled liquid, 20 g of glucose, 20 g of agar, and distilled water was added to 1 L) and cultured at 28°C for 48 hours. The isolated bacteria were cultured with PD liquid medium (containing 200g potato boiled liquid, 20g glucose, and distille...

Embodiment 2

[0062] Isolation, purification and identification of Safenour

[0063] Inoculate 10 μl of preserved Bacillus amyloliquefaciens WH3 strain into 10 ml sterilized PD medium, and cultivate overnight at 28° C. with shaking at 200 rpm. Then inoculate 2.5ml of fresh culture into a 1L Erlenmeyer flask containing 250ml of fresh PD medium, culture at 28°C or 37°C, and shake at 200rpm for 48h. Generally, 4 Erlenmeyer flasks are used to grow 1 L cultures at a time. Then the supernatant was collected by centrifugation at 8000g for 10min, and the antibacterial active substance was extracted by "two-step extraction method". After alcohol extraction, the n-butanol phase was collected by centrifugation at 8000 g for 20 min. The obtained n-butanol ester phase was evaporated to dryness at 65° C. with a vacuum rotary evaporator, and then dissolved with 10 ml of distilled water. After the solution obtained after dissolution was centrifuged to remove the precipitate, the supernatant was further ...

Embodiment 3

[0065] A kind of application of cyclic peptide Safenour in the oral insulin preparation (medicine) of preparation treatment or prevention diabetes, its steps are:

[0066] 1, a kind of preparation method of Bacillus amyloliquefaciens WH3 bacterial strain, its steps are:

[0067] (1) Screening and isolation of Bacillus amyloliquefaciens WH3:

[0068] Rapeseed seedlings at the three-leaf stage were randomly selected from the rapeseed field of Hubei Academy of Agricultural Sciences in Wuhan City, Hubei Province, China, and the roots were separated after washing. Rinse the outer surface of the root of rapeseed with tap water, rinse it with sterile water for 5 times, then immerse it in 70% (V / V) ethanol for 30 seconds, take it out, rinse it with sterile water for 5 times, and cut it with sterile scissors. The rice roots were cut into pieces about 1 mm in size, and then placed on PDA medium (containing 200 g of potato boiled liquid, 20 g of glucose, 20 g of agar, and distilled wate...

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Abstract

The invention discloses an application of Safenour cyclopeptide in an oral insulin medicine for treating diabetes, which comprises the following steps: 1) Safenour cyclopeptide and insulin are combined to have a protection effect; 2) after combining Safenour with insulin, the anti gastrointestinal tract acidic environment and digestive enzyme such as pepsin and trypsin after oral administration of insulin can be degraded; 3) absorption of intestinal tract on insulin can be promoted after combining Safenour with insulin; and 4) the administration is carried out after combining Safenour with insulin, the Safenour cyclopeptide has obvious diabetes and hpyerglycemic treatment effect. The security is high, the Safenour preparation is simple, and the problems that oral administration of insulin is easily degraded, so that unable oral administration and inconvenient carrying are provided can be solved. The oral insulin preparation which takes Safenour as an auxiliary material is easily carried, the usage is convenient, the problems of instability of natural insulin and quick-acting insulin analogues under acidic environment, twice injection on morning and evening everyday can be solved, and the Safenour cyclopeptide can be used for treating diabetes, and has good hpyerglycemic effect. The anti gastrointestinal tract degradating effect of the oral administration of insulin is good, and the Safenour cyclopeptide enables large scale production.

Description

technical field [0001] The invention belongs to the field of diabetes drug treatment, and more specifically relates to the application of a cyclic peptide Safenour in oral insulin preparations (medicines) for treating or preventing diabetes. Background technique [0002] With the development of biotechnology and genetic engineering, peptide and protein drugs are increasing and their uses are becoming wider and wider. The common characteristics of these drugs are high drug efficacy, short biological half-life, large molecular weight, and most of them have good water solubility, so they are not easy to pass through biomembranes. And because it is easily destroyed by gastrointestinal enzymes, it is mostly administered by injection at present. Because it is easy to be degraded by enzymes in the body, the biological half-life is relatively short, only a few minutes to ten minutes, and repeated injections are required. In order to overcome frequent The inconvenience and limitatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/12A61K38/28A61K47/42A61P3/10
Inventor 祁高富高振秋苏纪宇
Owner 武汉光谷世傲生物科技有限公司
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