Technology for extracting high-purity heparin sodium from intestinal mucosa by trypsin method

Abstract

The invention relates to the technical field of heparin sodium production, and provides a technology for extracting high-purity heparin sodium from intestinal mucosa by a trypsin method. The technology is characterized by adopting a fresh pig pancreas as the raw material to prepare a trypsin liquid and to extract the heparin sodium, therefore, utilization of artificial compositions is reduced greatly, the product nature is improved, the yield is high, and only about 1600 pig small intestines are needed to produce hundred million international units of the crude heparin sodium product; furthermore, as the trypsin liquid is adopted to extract the heparin sodium, peculiar smell in a production process can be reduced to a large extent, and the environmental protection is facilitated. According to the invention, a re-dissolution and re-precipitation step is added, so that the problem that the product purity is lower due to the fact that salt crystal is formed easily by a high-concentration alcohol and an eluent and is mixed in the heparin sodium product. The purity of the heparin sodium product processed by the technology is improved remarkably, and the titer of the heparin sodium is larger than 130 U / mg.

Description

technical field [0001] The invention relates to the technical field of heparin sodium production, in particular to a technique for extracting high-purity heparin sodium from intestinal mucosa by trypsin method. Background technique [0002] Heparin sodium, also known as heparin, is a natural anticoagulant substance containing acidic mucopolysaccharides containing sulfate groups. Heparin sodium is white or off-white powder, odorless, tasteless, hygroscopic, soluble in water, insoluble in organic solvents such as ethanol, acetone, and dioxane. [0003] Heparin sodium widely exists in mammalian liver, lung, and intestinal mucosa, and mostly exists in complexes combined with proteins. Enzyme hydrolyzed protein can separate heparin sodium, which is mucopolysaccharide containing sulfuric acid, amino group and aldonic acid. At pH 8-9, it is negatively charged, and can perform ion exchange with anion exchangers for rough separation, and the polysaccharide solution is precipitated ...

AI Technical Summary

Problems solved by technology

[0004] The defects of the traditional enzymatic hydrolysis production method of heparin sodium are: long production cycle, high energy consumption, low yield, the mucous membranes of 2800-3000 pig small intestines are needed to produce 100 million international units of heparin sodium crude product, the product purity is low, and the yield of heparin sodium is low. Potency is at most 80U / mg, and produces a large amount of intestinal residue and waste water, polluting the environment

2709 protease is a synthetic product, which reduces the naturalness of heparin sodium products, and the use of 2709 protease for enzymatic hydrolysis produces a large odor during the production process and pollutes the environment

Simultaneously the product yield of this method, purity are also lower