Enzymological detection method

A detection method and technology of enzymology, applied in the field of medical testing, can solve the problems of inability to apply clinical large-flow automatic analysis, high price, complicated operation, etc., and achieve the effects of high scientific value and economic value, simple operation and sensitive detection.
CN103207173AInactive Publication Date: 2013-07-17SHAOXING INST OF TECH COLLEGE OF ENG PEKING UNIV BIOENG CENT
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHAOXING INST OF TECH COLLEGE OF ENG PEKING UNIV BIOENG CENT
Publication Date
2013-07-17
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to the technical field of measuring and testing and discloses an enzymological detection method for detecting concentrations of citrulline, asymmetric dimethylarginine and the like in a reaction system by ATP (adenosine triphosphate) detection. According to the basic principle of reaction, ADMA (asymmetric dimethylarginine) is hydrolyzed by dimethylarginine dimethylaminohydrolase to obtain citrulline; in the presence of ADP (adenosine diphosphate) and Mg2+, the citrulline is subjected to coupling catalysis through ornithine carbamoyl transferase and carbamyl phosphokinase to finally obtain ornithine, carbon dioxide, ammonia, and ATP, and the released ATP is detectable by reagents. The enzymological detection method has the main advantages that reaction is sensitive, quick and highly accurate, the price is low, the ADMA or citrulline with the least concentration of 0.1 micromol / L can be detected, the detected concentration is approximate to the concentration of the ADMA in normal physiological serum and is far lower than the concentration of citrulline in the normal physiological serum, and a new available method for clinical ADMA detection is provided.
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Description

technical field

[0001] The invention relates to the technical field of medical testing, in particular to an enzymatic detection method using ATP chemiluminescence method for detection. Background technique

[0002] Asymmetric dimethylarginine (ADMA) is a methylated protein degradation product, it is a nitric oxide synthase inhibitor, it can competitively inhibit the activity of nitric oxide synthase, and reduce the production of nitric oxide , and can directly induce oxidative stress, as an endogenous relaxation factor, nitric oxide has the function of relaxing blood vessels, and plays an important role in diseases of the cardiovascular system. ADMA widely exists in human tissues, cells, plasma, and urine. It is mainly produced by the methylation of intracellular proteins under the action of arginine methyltransferase, and then hydrolyzed. 80% of ADMA in the human body ADMA is metabolized and inactivated by dimethylarginine dimethylamine hydrolase (DDAH), and only a small p...

Claims

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