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Fluorescent probe compound and its preparation method and use

A technique for fluorescent probes and compounds, applied in the fields of fluorescent probe compounds and their preparation and use, can solve the problems of application limitation, complex sample pretreatment process, and high detection cost, and achieve high selectivity, low analysis cost, and high sensitivity Effect

Inactive Publication Date: 2013-08-07
CHANGZHOU JINTAN BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Traditional palladium detection methods usually include atomic absorption spectrometry, plasma emission spectrometry, solid-phase microextraction-high performance liquid chromatography, X-ray fluorescence method, etc., but these methods often require complex sample pretreatment processes and special procedures. Trained personnel operate, and the detection cost is high, so the application is greatly limited

Method used

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  • Fluorescent probe compound and its preparation method and use
  • Fluorescent probe compound and its preparation method and use
  • Fluorescent probe compound and its preparation method and use

Examples

Experimental program
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Embodiment 1

[0028] In a 50ml dry round-bottomed flask, add 4-formylfluorescein (formula B) (0.36g, 1mmol) and anhydrous acetonitrile (15.0mL), stir to dissolve with a magnetic stirrer, and then dropwise add 2-aminobenzene Diphenylphosphine (DPPA) (0.305 g, 1.1 mmol) in acetonitrile (15 mL). After the addition, the reaction was carried out at room temperature for 24 hours. During the reaction, a large amount of white precipitates were produced. The reaction was filtered to obtain a white solid product, which was washed twice with acetonitrile and diethyl ether respectively. The above solid product was recrystallized with 5 mL of dichloromethane / petroleum ether (1:5, volume ratio) to obtain 0.485 g of pure white powder (A), yield: 78%.

[0029] Preparation of standard working curve:

[0030] Dissolve 7.19 mg of the fluorescent probe (A) prepared above in 1 mL of a 1:1 (volume ratio) aqueous solution of water and dimethylformamide to make 1×10 -2 M colorless solution, take 100L of the abov...

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Abstract

The invention discloses a fluorescent probe compound and its preparation method and use. The fluorescent probe compound has a general formula A, wherein in the general formula A, X1 and X2 respectively represent H and Cl. The fluorescent probe compound has high selectivity, high sensitivity and strong practicality and provides an important approach for fluorescent signal enhancement analysis of small and trace amounts of palladium compounds.

Description

technical field [0001] The present invention relates to a fluorescent probe compound and its preparation method and use. Background technique [0002] Palladium materials and palladium compounds play an important role in the field of material and chemical catalysis synthesis. Palladium metal materials are widely used in dental materials, gasoline combustion catalysts, electronic products and jewelry fields; various palladium compounds are used as excellent catalysts in the synthesis of fine chemicals. and widely used in drug synthesis. As a rare thiophilic transition metal, palladium compounds have serious harmful effects on human health due to their ability to combine with biological macromolecules such as amino acids, thioproteins and DNA in organisms. Recent studies have demonstrated that palladium compounds can even catalyze the Suzuki-Miyaura reaction in living cells [R.M.Yusop, A.Unciti-Broceta, E.M.V.Johansson, R.M.Sánchez-Martín and M.Bradley, Nat.Chem.2011, 3 , 23...

Claims

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Application Information

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IPC IPC(8): C07F9/6561C09K11/06G01N21/64G01N33/52
Inventor 曾宪顺
Owner CHANGZHOU JINTAN BIOTECH CO LTD