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Method for treating blue-green algae by utilizing microbial flocculant and hydrolyzed salt

A microbial flocculant and microbial technology, applied in the direction of microbial-based methods, biochemical equipment and methods, biological water/sewage treatment, etc., can solve the problems of secondary pollution, high treatment costs, low capture efficiency, etc., and achieve strong Adsorption, the effect of avoiding secondary pollution

Inactive Publication Date: 2013-08-28
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical algae removal method is based on the destruction of algae cells by chemical reagents to inhibit biological activity to remove algae. The chemical method kills algae quickly, but the cost is high, and sometimes it will cause secondary pollution and other problems; the mechanical method is based on "taking out" According to the idea, the use of algae removal equipment to catch algae can reduce water body source pollution. Due to the small size of cyanobacteria cells, they are evenly distributed in the water body, the capture efficiency is low, and the treatment cost is high; ecological restoration uses the principle of nutrient competition. Create artificial engineering to inhibit the growth of blue-green algae, which requires a large investment and slow results

Method used

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  • Method for treating blue-green algae by utilizing microbial flocculant and hydrolyzed salt
  • Method for treating blue-green algae by utilizing microbial flocculant and hydrolyzed salt
  • Method for treating blue-green algae by utilizing microbial flocculant and hydrolyzed salt

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Use L 25 (5 2 ) to optimize the flocculation conditions of Microcystis aeruginosa. Its factor level table is shown in Table 1. The flocculation test is as follows: take the algae liquid of Microcystis aeruginosa and place it in a conical flask, add a certain amount of FeCl 3 , shaking at 30°C and 150 rpm for 20 minutes, then adding different amounts of microbial flocculants to it, shaking for 10 minutes under the same conditions as above, standing for 30 minutes, filtering, and the filtrate was used for subsequent determinations. The evaluation indexes of the orthogonal test are COD, flocculation rate and pH.

[0022] Table 1 Orthogonal test factor level table

[0023]

[0024] Orthogonal test results are shown in Table 2, Fe 3＋ (A) has a greater effect on flocculation of Microcystis aeruginosa than microbial flocculants (B). The best combination of Microcystis aeruginosa flocculation is A 5 B 1 , namely Fe 3＋ The dosage is 0.02g / 200mL algae liquid, and th...

Embodiment 2

[0029] Take 500mL Microcystis aeruginosa and put them in 6 Erlenmeyer flasks, 3 of which are first added with microbial flocculant, shaken at 30°C and 150rpm for 10min, then add FeCl 3 , shaking at 30°C and 150rpm for 20 minutes, then standing for 30 minutes; the other three were first added with FeCl 3 , shake at 30°C and 150rpm for 20min, then add biological flocculant, shake at 30°C and 150rpm for 10min, and observe after standing for 30min. Paste double-sided tape on the SEM sample stage, take the flocs of Microcystis aeruginosa and evenly spread on the double-sided tape, air-dry, spray gold, and observe by SEM. Its electron microscope photo is attached figure 1 shown.

[0030] Add microbial flocculants to the culture medium of Microcystis aeruginosa first, and the microbial flocculants will disperse evenly in the algae liquid, and then add FeCl 3 Hydrolysis produces positively charged hydroxyl iron ions, which are adsorbed with the microbial flocculant to make the micr...

Embodiment 3

[0033] According to the optimized conditions in Example 1, take 4L of Microcystis aeruginosa liquid (pH=9.15) and put it in a 5L reagent bottle, add 40ml of microbial flocculant while stirring, stir for 10min, and then add Fe 3+ 0.02g, stir for 20min, let stand for 30min. The flocculated Microcystis aeruginosa was salvaged by a strainer, and the filtrate was used for analysis of physical and chemical properties. The flocculation effect is as attached figure 2 As shown, the changes in the physical and chemical properties of the cyanobacteria algae liquid are shown in Table 3.

[0034] Table 3 Changes of physical and chemical indicators of culture solution after flocculation of cyanobacteria

[0035]

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Abstract

The invention discloses a method for treating blue-green algae by utilizing a microbial flocculant and hydrolyzed salt. The method comprises the steps of: adding the microbial flocculant prepared by bacillus mucilaginosus into blue-green algae outbreak water body and hydrolyzed salt into blue-green algae outbreak water body, carrying out the synergistic effect of the microbial flocculant and hydrolyzed salt to flocculate blue-green algae so as to float the blue-green algae flocculating constituent on a liquid surface; fishing out and collecting the floating blue-green algae flocculating constituent, wherein the addition amount of the microbial flocculant is 0.05% to 10%, and the addition amount of the hydrolyzed salt is 0.005% to 1%. The method has the advantages that (1) the blue-green algae is flocculated by the microbial flocculant and hydrolyzed salt, the flocculating constituent floats on the water level so as to facilitate fishing; (2) by fishing out the blue-green algae flocculating constituent, the content of nitrogen and phosphorous in the water body can be reduced, the endogenous pollution can be purified, and the secondary outbreak of the blue-green algae can be restrained; (3) the microbial flocculant has strong adsorption effect on the hydrolysis of the metal ions, so that the residual metal ions in the water body can be reduced to an extremely low level so as to avoid secondary pollution; and (4) the blue-green algae flocculating constituent contains a great deal of organic matters which can be subjected to resource utilization.

Description

technical field [0001] The invention relates to a method for flocculating and collecting blue-green algae by using a microbial flocculant and hydrolyzed salt, which belongs to the field of environmental protection. Background technique [0002] Algal blooms in natural water bodies have become a global environmental disaster. Excessive blooms of algae have deteriorated water quality, seriously endangered water ecology, fisheries and landscape functions, and even threatened the safety of drinking water. [0003] For the control of cyanobacteria outbreak water, a variety of methods for controlling algae blooms have been studied at home and abroad, such as chemical methods, mechanical methods, ecological restoration methods and physical methods. The chemical algae removal method is based on the destruction of algae cells by chemical reagents to inhibit biological activity to remove algae. The chemical method kills algae quickly, but the cost is high, and sometimes it will cause ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C12P1/04C12R1/07
Inventor 连宾郝建朝
Owner NANJING NORMAL UNIVERSITY