Method for preparing walnut seed kernel oil by adopting brine enzymatic method
An enzymatic preparation, walnut technology, applied in the direction of fat oil/fat production, fat production, etc., can solve the problems of low extraction rate, impact on oil quality, high energy consumption, etc., and achieve high extraction rate, rich nutritional value, and oil quality. high effect
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Embodiment 1
[0023] (1) Weigh 20g of walnut kernels (with an oil content of 60%~70%), dry them at about 40°C, and grind them into a slurry with a sesame oil refiner;
[0024] (2) Add 1.6g of sodium chloride, 0.16g of α-amylase (activity ≥ 3700 activity units / g), 2.0g of cellulase (activity 10000u / g) into the slurry, then add 100mL of distilled water and stir well mixed;
[0025] (3) The mixed system was stirred and enzymolyzed at 50°C for 2 hours to obtain the enzymolyzed product;
[0026] (4) Centrifuge the enzymatic hydrolysis product at a temperature of 15°C and a speed of 6000r / min for 15 minutes; collect the centrifuged product in the upper layer, which is pure natural walnut seed oil, and the extraction rate is 78.93%.
Embodiment 2
[0028] (1) Weigh 20g of walnut kernels (with an oil content of 60%~70%), dry them at about 50°C, and grind them into a slurry with a sesame oil refiner;
[0029] (2) Add 2.0g sodium chloride, 0.2g α-amylase (activity ≥ 3700 activity units / g), 2.4g cellulase (activity 10000u / g) into the slurry, then add 120mL distilled water and stir well mixed;
[0030] (3) The mixed system was stirred and enzymolyzed at 55°C for 3 hours to obtain the enzymolyzed product;
[0031] (4) Centrifuge the enzymatic hydrolysis product at a temperature of 20°C and a rotational speed of 7000r / min for 20 minutes; collect the centrifuged product in the upper layer, which is pure natural walnut seed oil, and the extraction rate is 80.35%.
Embodiment 3
[0033] (1) Weigh 20g of walnut kernels (with an oil content of 60%~70%), dry them at about 60°C, and grind them into a slurry with a sesame oil refiner;
[0034] (2) Add 2.4g sodium chloride, 0.24g α-amylase (activity ≥ 3700 activity units / g), 2.8g cellulase (activity 10000u / g) to the slurry, then add 140 mL of distilled water, Stir to mix well;
[0035] (3) The mixed system was stirred and enzymolyzed at 60°C for 4 hours to obtain the enzymolyzed product;
[0036] (4) Centrifuge the enzymatic hydrolysis product at a temperature of 25°C and a speed of 8000r / min for 30 minutes; collect the centrifuged product in the upper layer, which is pure natural walnut seed oil, and the extraction rate is 79.06%.
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