A mung bean protein peptide having ACE inhibitory activity and a preparation method and applications thereof

A mung bean protein and activity-inhibiting technology, which is applied in the field of high value-added deep processing and utilization of agricultural by-products, can solve the problems of low product efficacy, retention, unfavorable end product development, etc., to facilitate protein sedimentation, promote centrifugal separation, and significant biological activity Effect

Active Publication Date: 2015-01-14
广州合诚实业有限公司
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0006] However, at present, the preparation technology of mung bean protein source ACE inhibitory peptide is still in the laboratory research stage, focusing on the enzymatic hydrolysis process, preliminary exploration of product activity and identification of active peptides. Some key link technologies have not yet been solved, and industrial scale preparation technology has not yet been formed. There are no related products on the market; on the other hand, the efficacy of the researched and developed products is low, mainly manifested in the fact that the IC50 of ACE inhibitory activity is much greater than 100 μg / mL, which is not conducive to the development of terminal products with significant efficacy

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  • A mung bean protein peptide having ACE inhibitory activity and a preparation method and applications thereof

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Experimental program
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Effect test

Embodiment 1

[0053] (1) Dispersion and dissolution of mung bean protein powder: Mung bean protein powder (64.8% protein content, 9.6% moisture content, the same below) was prepared as mung bean protein slurry with 45°C deionized water equivalent to 10 times the mass of mung bean protein powder, The slurry was dispersed and mixed for 20 min at a shear rate of 2000 rpm.

[0054] (2) Cellulase enzymatic hydrolysis and enzyme inactivation: adjust the pH value of the mung bean protein slurry dispersed and mixed in step (1) to 4.0 with a hydrochloric acid solution with a concentration of 3 mol / L, and add fiber equivalent to 0.5% of the mass of mung bean protein powder Suzyme (Type B, Guangzhou Yulibao Biotechnology Co., Ltd., the same below), after 1.5h of enzymolysis at 45°C with constant temperature stirring (speed 36rpm), the temperature was raised to 90°C for 15min to inactivate the enzyme.

[0055] (3) Centrifugal separation, washing with water to remove impurities: cool the mung bean prote...

Embodiment 2

[0061] (1) Dispersion and dissolution of mung bean protein powder: Prepare mung bean protein powder with 55°C deionized water equivalent to 6 times the mass of mung bean protein powder into mung bean protein slurry, and disperse and mix the slurry for 10 minutes at a shear speed of 3000 rpm.

[0062] (2) Cellulase enzymatic hydrolysis and enzyme inactivation: adjust the pH value of the mung bean protein slurry dispersed and mixed in step (1) to 3.5 with a hydrochloric acid solution with a concentration of 4mol / L, and add fiber equivalent to 1% of the mass of mung bean protein powder Sulfase, after enzymolysis at 55°C with constant temperature stirring (36rpm speed) for 1 hour, the temperature was raised to 85°C and kept for 20 minutes to inactivate the enzyme.

[0063] (3) Centrifugal separation, washing with water to remove impurities: cool the mung bean protein slurry after the enzyme inactivation in step (2) to below 65°C, centrifuge at 6000rpm for 10min, and take the precip...

Embodiment 3

[0069] (1) Dispersion and dissolution of mung bean protein powder: prepare mung bean protein powder with 50°C deionized water equivalent to 8 times the mass of mung bean protein powder into a mung bean protein slurry, and disperse and mix the slurry for 15 minutes at a shear speed of 2400 rpm.

[0070] (2) Cellulase enzymatic hydrolysis and enzyme inactivation: adjust the pH value of the mung bean protein slurry dispersed and mixed in step (1) to 4.5 with a hydrochloric acid solution with a concentration of 1mol / L, and add fiber equivalent to 0.1% of the mass of mung bean protein powder Sulfase, after enzymolysis at 50°C with constant temperature stirring (36rpm speed) for 2h, the temperature was raised to 100°C and kept for 10min to inactivate the enzyme.

[0071] (3) Centrifugal separation, washing with water to remove impurities: cool the mung bean protein slurry after the enzyme inactivation in step (2) to below 65°C, centrifuge at 5000rpm for 15min, and take the precipitat...

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Abstract

The present invention discloses a mung bean protein peptide having ACE inhibitory activity and a preparation method and applications thereof. According to the invention, the preparation method comprises: mixing water and mung bean protein powder to prepare mung bean protein slurry, after the pH of the slurry is adjusted to 3.5-5, adding cellulase for hydrolysis at 45-55 DEG C, after the enzyme hydrolysis, deactivating the cellulose, centrifugating, and washing with water to remove impurities; and then adjusting the pH to be 8-9, adding alkaline protease for hydrolysis at 50-60 DEG C, after the enzyme hydrolysis, deactivating the alkaline protease and obtaining the mung bean protein peptide having ACE inhibitory activity after coarse filtration, fine filtration and nanofiltration, and drying. According to the invention, the preparation method is energy efficient and convenient in operation, product quality and production efficiency are improved, and the obtained protein peptide has the ACE inhibitory activity of 42.8-59.6[mu]g / mL in terms of IC50, and can be widely used as a health food, or materials general food, in particular for the preparation of antihypertensive products assist.

Description

technical field [0001] The invention belongs to the technical field of high value-added deep processing and utilization of by-products of agricultural products, and particularly relates to a mung bean protein peptide with ACE inhibitory activity and its preparation method and application. Background technique [0002] Hypertension is a disease that is extremely harmful to human health. Statistics show that there were about 1 billion hypertensive patients in the world in 2010, and it will reach 1.56 billion in 2025. High topril, perindopril, trandolapril, etc.) are convenient to take, have direct effects, and have rapid onset but large side effects; Chinese medicines have small side effects and mild effects, but slow onset and are inconvenient to take; therefore, looking for Efficient and safe treatment of hypertension has become an important issue concerned with human health. [0003] Modern scientific research shows that active peptides obtained from animal and plant prot...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06A23L1/305
Inventor 周雪松曾建新蒋文真
Owner 广州合诚实业有限公司
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