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Bacillus calmette-guerin compound polysaccharide, and preparation method and use thereof

A composite polysaccharide and BCG technology, applied in biochemical equipment and methods, microorganism-based methods, drug combinations, etc., can solve the problem of no BCG polysaccharide nucleic acid research, etc., and achieve convenient administration methods and good clinical efficacy. , the effect of a wide range of indications

Inactive Publication Date: 2013-09-18
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the conditions and time constraints at that time, there was no further research on the components of BCG polysaccharide nucleic acid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0020] A preparation method of BCG complex polysaccharide, comprising the following steps:

[0021] (1) Continue to inoculate the subculture containing the standard strain of BCG in Sutong medium for expansion culture until it grows into a milky white to dark orange pellicle;

[0022] (2) Crude extraction of complex polysaccharides: extract the above culture by boiling, centrifuge to remove the precipitate, and use a polar organic solvent to precipitate complex polysaccharides at 2-6°C to obtain crude complex polysaccharides;

[0023] (3) Refining of complex polysaccharides: After the crude complex polysaccharides are treated with anion exchange column and ultrafiltration, freeze-drying to obtain refined complex polysaccharides, that is, BCG complex polysaccharides.

[0024] As a further solution: step (1) specifically includes the following steps:

[0025] 1) Prepare Sutong liquid medium: mix 4.0g asparagine, MgSO 4 .7H 2 O 0.5g, citric acid 2.0g, ferric ammonium ...

Embodiment

[0033] (1) Preparation of BCG compound polysaccharide injection: Dissolve BCG compound polysaccharide in water for injection at 35°C-45°C, adjust the pH value to 7.0-7.4, add water to the full amount, and use ultrafilter to remove heat source, PVDF After membrane filtration and packaging, autoclave;

[0034] (2) Preparation of BCG complex polysaccharide suspension: Dissolve BCG complex polysaccharide with water for injection at 35°C-45°C, adjust the pH value to 7.0-7.4, add water to the full amount, use an ultrafilter to remove heat source, high pressure sterilization;

[0035] (3) Preparation of BCG compound polysaccharide tablet: Mix BCG compound polysaccharide thoroughly and grind it in a high-speed mill for micronization, and mix it with fine powder, disintegrating agent, binder and 2 / 3 The amount of disintegrating agent is fully mixed evenly, at 3-4kg / cm 2 Pressure tablet.

[0036] As a further solution: the pharmaceutical composition of BCG complex polysaccharide is u...

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Abstract

The invention discloses bacillus calmette-guerin compound polysaccharide, and a preparation method and use thereof. The preparation method comprises the steps of extracting bacillus calmette-guerin compound polysaccharide from a bacillus calmette-guerin cell wall by using a biochemical method; firstly, continuing to inoculate subculture containing a bacillus calmette-guerin standard strain on Sutong culture medium to carry out extended cultivation until mycoderm from milk white to dark orange grows; carrying out compound polysaccharide crude extraction and compound polysaccharide purification. The prepared bacillus calmette-guerin compound polysaccharide comprises arabinose, mannose, glucoside and galactose; the molecular weight is 2000-36000 Daltons. The bacillus calmette-guerin compound polysaccharide can simulate an immune system of people; activation of a plurality of immune cells and generation of cell factors are facilitated, so as to play the immunomodulatory effect. The preparation has no toxic and side effect on application to a human body, and can be used for preventing influenza, asthma, an anaphylactic disease and intracellular infectious diseases, and assisting auxiliary anti-tumor treatment.

Description

technical field [0001] The invention relates to a method for extracting BCG complex polysaccharide from BCG cell wall and application of the extracted BCG complex polysaccharide. Background technique [0002] BCG polysaccharide nucleic acid is a new type of biological response modifier developed by the former Hunan Medical University from the 1970s to the 1990s. A large number of studies have shown that this product has strong immunoregulatory pharmacological effects such as anti-allergy, anti-intracellular infection, and anti-tumor, and it is clinically effective in preventing and treating colds and asthma. Its main mechanism of action is: (1) Inducing Th 0 Cells differentiate into Th 1 It promotes the secretion of γ-interferon (IFN-γ) and interleukin-2 (IL-2), stimulates B cells to synthesize IgG2a, inhibits the synthesis of IgG1, and has immune regulation and anti-allergic effects; Nucleus-macrophage function, mice injected with this product intraperitoneally, the numb...

Claims

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Application Information

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IPC IPC(8): C12P19/04C08B37/00A61K31/715A61K45/00A61P37/02A61P11/00A61P11/06A61P37/08A61P31/00A61P35/00C12R1/32
Inventor 高戈高梓博高洁生
Owner CENT SOUTH UNIV
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