Swine immunopotentiator and its preparation method
An immune enhancer and extract technology, applied in the field of pig immune enhancer and its preparation, can solve problems such as stress reaction of farmed animals, economic loss of farmers, impact on human health, etc.
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[0023] Preparation of phytase-producing Bacillus subtilis bacterial powder:
[0024] 1. Obtaining of fermented liquid: Obtain phytase-producing Bacillus subtilis fermented liquid by step-by-step expansion cultivation of slanted strains;
[0025] (1) First-level seed culture: Inject the preserved phytase-producing Bacillus subtilis slant strain into a 500-ml shaker flask with a medium volume of 100 ml, a rotary shaker at 160 rpm, and a culture temperature of 30 ℃, the incubation time is 24 hours;
[0026] (2) Secondary seed cultivation: Put the first-grade seeds into 500 ml second-grade seed shake flasks according to the inoculation amount of 10%, and the cultivation conditions are the same as the first-grade seeds;
[0027] (3) Tertiary seed cultivation: Inoculate the secondary seeds with 10% inoculum into a 5000ml tertiary seed shaker flask, the medium capacity is 1000ml, and the rotary shaker is 120 rpm / Minutes, the culture is stable at 30°C, and the culture time is 24 h...
Embodiment 1
[0036] An immune enhancer for pigs and a preparation method thereof, comprising the following raw materials in parts by weight: an immune enhancer for pigs and a preparation method thereof, consisting of the following raw materials in parts by weight: 12 parts of yeast β-glucan, poplar flower extract 10 parts of bamboo shoot shell extract, 15 parts of phytase-producing Bacillus subtilis powder, 6 parts of propolis, 2 parts of γ-aminobutyric acid, 1 part of mannan oligosaccharide, and 3 parts of chlorogenic acid.
[0037] The preparation method of the poplar flower extract is as follows:
[0038]Take the dried poplar flowers and crush them, pass through 40 meshes, put them in a container, add 5 times the amount of water, control the temperature at 90°C for 1 hour, filter the filtrate, add ethanol to 45% for precipitation, and concentrate the supernatant in vacuo Then freeze-dry to obtain poplar flower extract.
[0039] The preparation method of the bamboo shoot husk extract: t...
Embodiment 2
[0043] An immune enhancer for pigs and a preparation method thereof, comprising the following raw materials in parts by weight: an immune enhancer for pigs and a preparation method thereof, consisting of the following raw materials in parts by weight: 18 parts of yeast β-glucan, poplar flower extract 15 parts, 18 parts of bamboo shoot shell extract, 20 parts of phytase-producing Bacillus subtilis powder, 8 parts of propolis, 3 parts of γ-aminobutyric acid, 2.5 parts of mannan oligosaccharides, and 4 parts of chlorogenic acid.
[0044] The preparation method of the poplar flower extract is as follows:
[0045] Take dried poplar flowers and crush them, pass through 40 meshes, put them in a container, add 6 times the amount of water, control the temperature at 95°C for 1.25 hours, filter and get the filtrate, then add ethanol to 45% concentration for precipitation, the obtained supernatant, vacuum Concentrated and then freeze-dried to obtain poplar flower extract.
[0046] The p...
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