Bdellovibrio bacteriovorus preparation, and fermentation method and applications thereof

A fermentation method and technology of Bdellovibrio, which is applied in the field of fermentation, can solve the problems of low content of Bdellovibrio preparations and low cracking activity, achieve the effects of shortening the fermentation cycle, high activity, and solving excessive energy consumption

Inactive Publication Date: 2013-09-25
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the problems of low content and low cracking activity of Bdellovibrio preparations prepared by existing methods, the primary purpose of the present invention is to provide a fermentation method for Bdellovibrio preparations

Method used

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  • Bdellovibrio bacteriovorus preparation, and fermentation method and applications thereof
  • Bdellovibrio bacteriovorus preparation, and fermentation method and applications thereof
  • Bdellovibrio bacteriovorus preparation, and fermentation method and applications thereof

Examples

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Effect test

Embodiment 1

[0051] Bacillus subtilis (strain number: GIM1.136, obtained from the Guangdong Provincial Culture Collection Center for Microorganisms) and Escherichia coli (strain number: GIM1.137, obtained from the Guangdong Provincial Culture Collection Center for Microorganisms) were used as The host bacterium prepares Bdellovibrio preparation A and Bdellovibrio preparation B, and the specific process is as follows:

[0052] (1) Preparation of Bacillus subtilis suspension

[0053] Inoculate Bacillus subtilis in nutrient broth medium (peptone 10g, beef extract powder 3g, sodium chloride 5g, pH 7.4±0.2), and place it in a shaker at 33°C for 18h to make it in the logarithmic growth phase , the culture solution was centrifuged at 5000rpm for 25min at 4°C, the precipitate was retained and the supernatant was discarded, and sodium potassium phosphate buffer (concentration: 0.2mol / L, p H7.6), resulting in a concentration of 10 19 cfu / mL of the Bacillus subtilis suspension, then placed in a ref...

Embodiment 2

[0062] Using Bacillus natto (strain number: CGMCC1.1086, from the General Microbiology Center of the China Microbial Culture Collection Management Committee) and Escherichia coli as the host bacteria to prepare leech vibrio preparation A and leech vibrio preparation B, the specific process is as follows:

[0063] (1) Preparation of Bacillus natto suspension

[0064] Bacillus natto was inoculated into the nutrient broth medium (10g peptone, 3g beef extract powder, 5g sodium chloride, pH 7.4±0.2), and placed in a shaker at 35°C for 15h to keep it in logarithmic growth. The culture medium was centrifuged at 6000 rpm for 25 min at 4°C, the supernatant was discarded and the precipitation was retained, and sodium phosphate buffer (concentration 0.1 mol / L, pH 7.4) was added to the precipitation to obtain a concentration of 10 18 cfu / mL of Bacillus natto suspension, and then stored in a 2°C refrigerator for later use;

[0065] The same method as above, the preparation concentration i...

Embodiment 3

[0073] Bifidobacterium bifidum (strain number: GIM1.169, from the Guangdong Provincial Microbial Culture Collection Center) and Salmonella typhimurium were used as host bacteria to prepare Hirudovibrio preparation A and Hirudovibrio preparation B. The specific process is as follows:

[0074] (1) Preparation of Bifidobacterium bifidum suspension

[0075] Bifidobacterium bifidum was inoculated into PTYG medium, placed in a shaker at 37°C for 24h to make it in the logarithmic growth phase, the culture medium was centrifuged at 7000rpm for 20min at 8°C, the precipitate was retained and the supernatant was discarded. , adding potassium phosphate buffer (concentration 0.3mol / L, pH7.5) to the precipitate to obtain a concentration of 10 22 cfu / mL of Bifidobacterium bifidum suspension, and then stored in an 8°C refrigerator for later use;

[0076] The same method as above, using the nutrient broth medium to prepare a concentration of 10 22 cfu / mL Salmonella typhimurium suspension, st...

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Abstract

The invention discloses a bdellovibrio bacteriovorus preparation, and a fermentation method and applications thereof. The invention discloses a bdellovibrio bacteriovorus preparation and a fermentation method and application thereof. The fermentation method comprises the following steps of: (1) preparation of a host bacteria suspension; (2) preparation of a bdellovibrio bacteriovorus telotroch concentrated solution; and (3) preparation of the bdellovibrio bacteriovorus preparation. Host bacteria adopted in the fermentation method are Gram-positive bacteria which are beneficial or harmless to the environment, the method can keep and even improve the capability of bdellovibrio bacteriovorus for cracking certain gram-positive bacteria, and the bdellovibrio bacteriovorus preparation prepared by using the method has certain improvement on the cracking capability for pathogenic bacteria; and the prepared bdellovibrio bacteriovorus preparation can be directly used without more fermentation post treatment, and the use range of the preparation is greatly increased. The method relatively shortens the fermentation period at the same time of obtaining high-concentration bdellovibrio bacteriovorus bacterial solution so as to effectively solve the problems of over-high energy consumption and over-high productioncost caused by overlong fermentation time; and the obtained bdellovibrio bacteriovorus preparation has low cost and high activity.

Description

[0001] The patent application of the present invention is a divisional application with an application number of "201010270772.9", the application date of the original application is "August 31, 2010", the application number is "201010270772.9", and the name of the invention is "A leech vibrio preparation and its Fermentation methods and applications". technical field [0002] The invention relates to the technical field of fermentation, in particular to a leech vibrio preparation and a fermentation method and application thereof. Background technique [0003] At present, the main means of controlling and eliminating pathogenic bacteria are still various physical and / or chemical methods, including the use of various antibiotics. Both physical and / or chemical methods have obvious drawbacks. First, the large-scale abuse of antibiotics will lead to some side effects, such as drug resistance of pathogenic bacteria and inhibition of beneficial flora. Secondly, although the metho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/74A61P31/04C12R1/46C12R1/01
Inventor 蔡俊鹏陈小红
Owner SOUTH CHINA UNIV OF TECH
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