Bdellovibrio bacteriovorus preparation, and fermentation method and applications thereof
A fermentation method and technology of Bdellovibrio, which is applied in the field of fermentation, can solve the problems of low content of Bdellovibrio preparations and low cracking activity, achieve the effects of shortening the fermentation cycle, high activity, and solving excessive energy consumption
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Embodiment 1
[0051] Bacillus subtilis (strain number: GIM1.136, obtained from the Guangdong Provincial Culture Collection Center for Microorganisms) and Escherichia coli (strain number: GIM1.137, obtained from the Guangdong Provincial Culture Collection Center for Microorganisms) were used as The host bacterium prepares Bdellovibrio preparation A and Bdellovibrio preparation B, and the specific process is as follows:
[0052] (1) Preparation of Bacillus subtilis suspension
[0053] Inoculate Bacillus subtilis in nutrient broth medium (10g of peptone, 3g of beef extract powder, 5g of sodium chloride, pH7.4±0.2), and culture it in a shaker at 33°C for 18h, so that it is in the logarithmic growth phase , the culture solution was centrifuged at 5000rpm for 25min at 4°C, the precipitate was retained and the supernatant was discarded, and sodium potassium phosphate buffer (concentration: 0.2mol / L, pH7.6) was added to the precipitate to obtain a concentration of 10 19 cfu / mL of the Bacillus subt...
Embodiment 2
[0062] Bdellovibrio preparation A and Bdellovibrio preparation B were prepared with Bacillus natto (strain number: CGMCC1.1086, from the General Microbiology Center of China Microbiological Culture Collection Management Committee) and Escherichia coli as host bacteria. The specific process is as follows:
[0063] (1) Preparation of Bacillus natto suspension
[0064] Inoculate Bacillus natto in nutrient broth medium (peptone 10g, beef extract powder 3g, sodium chloride 5g, pH 7.4±0.2), and culture it in a shaker at 35°C for 15h to make it grow logarithmically During the period, the culture solution was centrifuged at 6000rpm for 25min at 4°C, the precipitate was retained and the supernatant was discarded, and sodium phosphate buffer (concentration: 0.1mol / L, pH7.4) was added to the precipitate to obtain a concentration of 10 18 The suspension of Bacillus natto of cfu / mL is then placed in a refrigerator at 2°C for subsequent use;
[0065] The same method as above, the preparati...
Embodiment 3
[0073] Bdellovibrio preparation A and Bdellovibrio preparation B were prepared using Bifidobacterium bifidum (strain number: GIM1.169, sourced from Guangdong Microbial Culture Collection Center) and Salmonella typhimurium as host bacteria, respectively. The specific process is as follows:
[0074] (1) Preparation of Bifidobacterium bifidum suspension
[0075] Inoculate Bifidobacterium bifidum in PTYG medium, culture in a shaker at 37°C for 24 hours to make it in the logarithmic growth phase, centrifuge the culture solution at 8°C at 7000rpm for 20min, retain the precipitate and discard the supernatant , add potassium phosphate buffer (concentration: 0.3mol / L, pH7.5) to the precipitate to obtain a concentration of 10 22 cfu / mL Bifidobacterium bifidum suspension, and then placed in a refrigerator at 8°C for future use;
[0076] In the same way as above, use the nutrient broth medium to prepare a concentration of 10 22 The Salmonella typhimurium suspension of cfu / mL is stored i...
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