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A kind of preparation method of fish skin collagen for medical grade biological material

A technology of biomaterials and fish skin, applied in the preparation method of peptides, chemical instruments and methods, animal/human proteins, etc., can solve the problems of polluted environment, long processing time, and undiscovered problems, and achieve good biocompatibility, The effect of short processing time

Active Publication Date: 2015-12-23
烟台德胜海洋生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to statistics, only tens of thousands of tons of fish skins are discarded in the processing process every year, which pollutes the environment and wastes resources.
[0006] In the prior art, the skin of deep-sea fish is mainly used, and even only the skin of specific fish can be processed to obtain fish skin collagen for medical grade biomaterials. The operation steps are cumbersome, the processing time is long, and the obtained fish skin collagen Low protein content, high ash content, no good biocompatibility, narrow application range
In the current domestic and foreign research literature on the use of fish skin to extract collagen, there is also a processing technology that uses EDTA to pretreat the raw materials. The production process of this technology is seriously polluted and the extraction cycle is long. One week, hard to industrialize
[0007] There are many types of fish, and they live in different environments of seawater and freshwater, which makes the structure of fish skin different. So far, there has been no report on a complete and effective method for industrially preparing fish skin collagen for medical grade biomaterials.

Method used

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  • A kind of preparation method of fish skin collagen for medical grade biological material
  • A kind of preparation method of fish skin collagen for medical grade biological material

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Take 200g of fresh cod skin, remove the residual meat and fat, cut it into 1cm×1cm small pieces with a slicer, rinse with 20 times 4°C pure water for 30 minutes, repeat once, and drain. Put the fish skin into 3% NaCl solution at 4°C, the ratio of solid to liquid is 1:30 (w / w), stir for 40 hours, change the liquid every 10 hours, and drain. Wash with deionized water 4°C 1:20 (w / w) 4 times for 30 minutes each time, and drain the water.

[0034] Put the fish skin into 0.2M NaOH solution at 4°C, the ratio of solid to liquid is 1:30 (w / w), stir for 40 hours, change the liquid every 10 hours, and drain. Wash with deionized water at 4°C (1:30w / w) for 3 times, each time for 1 hour, until the pH is 7-8, and drain the water.

[0035] Put the washed fish skin into a 0.5M phosphoric acid solution at 4°C, stir with a solid-liquid ratio of 1:30, centrifuge at 10,000 rpm at 4°C, and take the supernatant for later use. Then add 0.5M phosphoric acid solution to the centrifuged precipita...

Embodiment 2

[0039] Take 200g of fresh tilapia skin, remove scales, residual meat and fat, cut into 1cm×1cm small pieces with a slicer, rinse with 20 times of 4°C pure water for 30 minutes, and repeat once, drain the water. Put the fish skin in 4% NaCl solution at 4°C, the ratio of material to liquid is 1:30 (w / w), stir for 36 hours, change the liquid every 8 hours, drain the water, and use deionized water at 4°C 1:20 (w / w) wash 4 times, 30min each time, drain the water.

[0040] Put the fish skin into 0.1M NaOH solution at 4°C, the ratio of solid to liquid is 1:30 (w / w), stir for 48 hours, change the liquid every 12 hours, and drain the water. Wash with deionized water at 4°C (1:30w / w) for 3 times, each time for 1 hour, until Ph7-8, and drain the water.

[0041] Put the washed fish skin into 0.5M acetic acid solution at 4°C, with a material-to-liquid ratio of 1:30, mash it with a tissue grinder, stir for 4 hours, centrifuge at 10,000 rpm at 4°C, and take the supernatant. Put the precipi...

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Abstract

The invention provides a preparation method of fish-skin collagen for a medical biomaterial. The preparation method provided by the invention comprises steps of raw material treatment, collagen extraction, extract purification and the like. By the adoption of the preparation method of fish-skin collagen for a medical biomaterial, non-collagen components of fish-skin can be fully and effectively removed; high-purity collagen with the content of obtained hydroxyproline being greater than or equals to 9%, the content of non-collagen being less than or equals to 1% and the content of ash being less than or equals to 1% is obtained; and fish resources can be utilized within a larger range. The preparation method has advantages of simple technology, easy operation, short processing time and obvious biocompatibility effect, and is suitable for industrial production.

Description

technical field [0001] The invention belongs to the field of preparation of biomedical materials, and in particular relates to a method for extracting natural macromolecule collagen which can be used as biomedical materials from fish skin. Background technique [0002] Collagen is the most abundant and important structural protein in animals. It is widely distributed in the skin, bones, cartilage, teeth, tendons, ligaments, and blood vessels of all multicellular animals from aquatic animals to vertebrates. It is the main support tissue and connective tissue. The components, accounting for about 25% of the total body protein, play a variety of functions in animals such as supporting organs, protecting the body, connecting, and nourishing. [0003] Most of the collagen fibers in mammals belong to type I collagen, and type I collagen consists of two α 1 Peptide chain and 1 α 2 The peptide chain forms a triple-helix superhelical structure through the α-helical structure. This...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K14/78C07K1/36C07K1/34C07K1/30
Inventor 王南平何兰曹俊郭休玉
Owner 烟台德胜海洋生物科技有限公司
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