Method for preparing diet food with natural biological active proteins of rice
A technology of biologically active protein and diet food, which is applied in the field of preparation of diet food, can solve the problems of high preparation cost, unfavorable large-scale popularization and utilization, etc., and achieves the effects of simple preparation, easy operation and implementation, and good economic benefits.
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Embodiment 1
[0029] 1) Using commercially available Beidahuang Green Food Delivery Co., Ltd. organic brown rice as raw material, crushed and sieved through a 40-mesh sieve to obtain rice noodle raw material. The rice noodle raw material is defatted with acetone. The rice noodle raw material (g) and acetone ( mL) The solid-liquid ratio is 1:3g / mL. After shaking for 2 hours, the precipitate is collected by centrifugation at 6000 rpm for 20 minutes, and placed in a fume hood to air dry to obtain defatted rice noodles.
[0030] 2) Extraction of total salt-soluble protein from rice: Weigh 15g of defatted rice flour and add 120mL of 1.0mol / L NaCl aqueous solution, mix well, shake and extract at 40℃ for 2h, centrifuge at 6000rpm for 20min to collect the supernatant, repeat step 2) once, combine Clear liquid to obtain total salt-soluble protein solution;
[0031] 3) Ethanol precipitation and impurity removal: Add absolute ethanol to the total salt-soluble protein solution in step 2), and the volume rat...
Embodiment 2
[0034] 1) Using commercially available Beidahuang Green Food Distribution Co., Ltd. organic brown rice as raw material, crushed and passed through a 50-mesh sieve to obtain rice noodle raw material. The rice noodle raw material is defatted with acetone. The rice noodle raw material (g) and acetone ( mL) The solid-liquid ratio is 1:3g / mL. After shaking for 2 hours, the precipitate is collected by centrifugation at 6000 rpm for 20 minutes, and placed in a fume hood to air dry to obtain defatted rice noodles;
[0035] 2) Extraction of total salt-soluble protein from rice: weigh 15g of defatted rice flour and add 150mL of 1.0mol / L NaCl aqueous solution, mix well, shake and extract at 30℃ for 2h, centrifuge at 6000rpm for 20min to collect the supernatant, repeat step 2) once, combine the supernatants To obtain a total salt-soluble protein solution;
[0036] 3) Ethanol precipitation and impurity removal: Add absolute ethanol to the total salt-soluble protein solution in step 2), and the ...
Embodiment 3
[0039] 1) Use commercially available fine defatted rice bran powder produced by Jiangxi Tianyu Oil Co., Ltd. as raw material as defatted rice flour;
[0040] 2) Extraction of total salt-soluble protein from rice: accurately weigh 15g of defatted rice flour and add 150mL of 1.0mol / L NaCl aqueous solution, mix well, shake and extract at 30℃ for 2h, centrifuge at 6000rpm for 20min to collect the supernatant, repeat step 2) once, combine Supernatant to obtain total salt-soluble protein solution;
[0041] 3) Ethanol precipitation and impurity removal: Add absolute ethanol to the total salt-soluble protein solution in step 2). The volume ratio of the total salt-soluble protein solution to the absolute ethanol is 1:2.5. At the same time, stir and mix with a magnetic stirrer. Precipitate at 4°C for 1 hour, centrifuge at 8000 rpm for 20 minutes, remove the precipitated part, collect the supernatant to obtain the biologically active protein solution;
[0042] 4) Post-processing ethanol remova...
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