Special multi-element trace element additive for biological culture

Abstract

The invention relates to a special multi-element trace element additive for biological culture. The multi-element trace element additive is especially suitable for the supplementation of multi-element trace elements in the culturing processes of a plurality of biologically active materials comprising plant cells, fungi and animals. The multi-element microbial element additive is named as a 'super power element', is obtained through chelating all trace elements required by organisms and ethylene diamine tetraacetic acid (EDTA) under high temperature reflux, and is a deep purple clear solution obtained after co-dissolving a ferrous salt, a magnesium salt, a zinc salt, a copper salt, a molybdenum salt, boric acid, a cobalt salt, a manganese salt, a cerium salt and a lanthanum salt and EDTA according to a certain ratio and carrying out boiling refluxing for 7h, and the clear solution is added to a biologic medium in a 0.01% proportion. The additive has the characteristics of high stability, wide pH tolerance characteristic, controlled release cell absorption and low cost, can effectively promote the healthy growth of cultured materials, improves the activities and the breeding speed, and has wide application values.

Description

technical field [0001] The invention relates to a biological culture additive, in particular to a special multi-element trace element additive for plant cells, fungi and animal cell culture and its application method. Background technique [0002] Organisms are composed of more than 60 elements. According to the content of the elements in the organism, they can be divided into two categories: macro elements and trace elements. Any element that accounts for more than one ten-thousandth of the total weight of an organism, such as carbon, hydrogen, oxygen, nitrogen, calcium, phosphorus, magnesium, sodium, etc., is called a constant element; any element that accounts for less than one ten-thousandth of the total body weight Elements, such as iron, zinc, copper, manganese, chromium, selenium, molybdenum, cobalt, fluorine, etc., are called trace elements (iron is also called semi-trace elements). The content of trace elements in the human body is really negligible, such as zinc ...

AI Technical Summary

Problems solved by technology

The additive can effectively solve the lack of trace elements in the cultivation process of plant cells, fungi and animal cells without changing the physical and chemical properties of the medium, help the culture to absorb trace elements slowly, and permanently improve the problem of lack of trace elements in the culture , to promote the healthy growth of cells and enhance their biological activity

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