Lactobacillus preparation for preventing and controlling late farmed shrimp ponds vibrio
A technology of Lactobacillus and Lactobacillus casei, applied in the field of applied microorganisms, can solve problems such as general mentioning, and achieve the effects of long validity period and strong environmental adaptability
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Embodiment 1
[0027] Prepare MRS agar and MRS broth medium, spare, the composition of MRS agar medium is as follows: peptone 10 g, beef extract 10 g, yeast extract 5 g, glucose 20 g, Tween 80 ml, K 2 HPO 4 2 g, sodium acetate 5 g, diammonium citrate 2 g, MgSO 4 7H 2 O 0.2 g, MnSO 4 4H 2 O 0.05 g, agar powder 15 g, add distilled water to 1000 ml, sterilize at 121°C for 15 min with an autoclave, and adjust the pH to 6.2-6.4. The composition of MRS broth medium is MRS agar medium minus agar powder. Activation of Lactobacillus casei (Lactobacillus casei) BFT-1 with MRS slant, preservation number: CCTCC No: M2011424, then inoculated in 100 ml of MRS broth medium, cultured at 30°C for 24 hours before use. Prepare 2216E solid medium: 5 g peptone, 1 g yeast extract, 0.1 g ferric phosphate, 15 g agar, add aged seawater to 1000 ml, adjust pH to 7.6, sterilize at 121°C for 20 min in an autoclave, pour Solid tablet. Mix the activated Vibrio anguillarum with sterile water and spread it on the 22...
Embodiment 2
[0030]1) Slant activation and shake flask culture of strains. Inoculate the Lactobacillus casei with the preservation number CCTCC No: M2011424 on the solid medium of MRS agar slant, cultivate it at 30°C for 24 hours to activate it, and then insert it into a shaker flask containing 100 ml of MRS broth medium. 30°C, incubate for 24 hours, set aside;
[0031] 2) Seed tank fermentation: put the MRS broth medium into the seed tank, steam high-temperature sterilization and cooling in the seed tank, and insert the shake flask bacteria in step (1) according to the inoculation amount of 0.5%; Start the mixer for 3 minutes for 1 hour to mix, temperature 30°C, tank pressure 0.06M Pa, cultivate for 24 hours under natural pH conditions, and set aside;
[0032] (3) Preparation of batch products: Load the batch production medium in the fermenter: yeast extract 0.8%, peptone: 0.9%, glucose 1.8%, sodium chloride 0.4%, calcium carbonate 0.9%, the rest is water, initial pH 7.0-7.4; steam high...
Embodiment 3
[0035] 1) Slant activation and shake flask culture of strains. The specific implementation is the same as step 1 of embodiment 2);
[0036] 2) Seed tank fermentation: put the MRS broth medium into the seed tank, steam high temperature sterilization in the seed tank and cool it down, add the shake flask bacteria in step (1) according to the inoculation amount of 1%; Start the mixer for 3 minutes for 1 hour to mix, temperature 30°C, tank pressure 0.06M Pa, cultivate for 24 hours under natural pH conditions, and set aside;
[0037] (3) Preparation of batch products: Fill the fermenter with batch production medium, yeast extract 1%, peptone: 1.2%, glucose 2.5%, sodium chloride 0.5%, calcium carbonate 1.2%, the rest is water, initial pH 7.0-7.4; steam high-temperature sterilization and cooling in the fermenter; put the seed solution in step (2) into the fermenter, the inoculation amount is 10% of the volume of the fermenter, and start the mixer for 3 minutes every 1h to mix. The ...
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