Method for measuring nitrites by surface enhanced Raman spectroscopy (SERS)

A surface-enhanced Raman and nitrite technology, applied in the field of analytical chemistry, to achieve the effects of easy availability of reagents, simple operation and high sensitivity

Inactive Publication Date: 2013-11-27
GUANGXI NORMAL UNIV
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The method of determining nitrite by surface-enhanced Raman spectroscopy has not been reported yet

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  • Method for measuring nitrites by surface enhanced Raman spectroscopy (SERS)

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[0019] The method for measuring nitrite with surface-enhanced Raman spectroscopy comprises the steps:

[0020] (1) Prepare a standard test system of nitrite with known concentration: add 100 μL 1.0 mol / L HCl solution, 100 μL 1.0×10 -5 mol / L Rh6G solution, and 0.5 μL, 20 μL, 50 μL, 80 μL, 120 μL 1.45 mmol / L nitrite standard solution, dilute to 1.0 mL with double distilled water, mix well, react at room temperature for 15 minutes, add to the test tube in turn 120 μL 1.0 mol / L NaCl solution, 300 μL 57 μg / mL nano-gold solution, dilute to 2.0 mL with twice distilled water;

[0021] (2) Prepare the blank control system without adding nitrite according to the method of step (1);

[0022] (3) Take the solutions of the standard test system and place them in quartz cuvettes respectively. On the DXR smart Raman spectrometer, set the instrument parameters laser power to 3.5mW, acquisition time to 2.0s, and scan to obtain the surface-enhanced Raman spectrometer of the system. Mann spect...

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Abstract

The invention discloses a method for measuring nitrites by surface enhanced Raman spectroscopy (SERS). The method comprises the following steps of: 1. preparing an analytic solution of nitrates with known concentrations, and measuring the SER scattering peak intensity value at 1506cm<-1> of the analytic solution to be I; 2. preparing a blank control system excluding nitrates, and measuring the SER scattering peak intensity value of the system to be I0; 3. computing delta I equal to I0 minus I; 4. formulating a working curve of the concentrations of nitrates based on delta I; 5. preparing an analytic solution of tested samples, measuring the SER scattering peak intensity value of the analytic solution to be the I sample, and computing the delta I sample equal to I0 minus I sample; and 6. computing the concentrations of nitrates in the tested samples according to the working curve. Compared with the existing methods, the method has the advantages that the method is simple and convenient to operate, and the reagent is accessible and has high sensitivity and good selectivity.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a method for measuring nitrite by surface-enhanced Raman spectroscopy. Background technique [0002] Nitrite can react with amines and amide compounds produced by protein decomposition in the human body to generate carcinogen nitrosamines, which can oxidize hemoglobin and lose the ability to transport oxygen, causing acute poisoning. In the process of food processing, nitrite is an important coloring and preservative. It has many beneficial functions for meat curing, but its usage should be strictly limited. Therefore, research on fast, simple and sensitive nitrite detection methods is of positive significance for controlling food safety and protecting human health. At present, there are many techniques for detecting nitrite, such as photometry, chromatography, fluorescence analysis, chemiluminescence, electrochemical method, resonance Rayleigh scattering spectroscopy and so ...

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Application Information

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IPC IPC(8): G01N21/65G01N1/38
Inventor 蒋治良董金超梁爱惠
Owner GUANGXI NORMAL UNIV
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