Method for detecting trace ochratoxin A (OTA) by adopting electrochemical aptamer sensor
An aptamer sensor, ochratoxin technology, applied in the direction of measuring devices, scientific instruments, instruments, etc., can solve the problem that the change of electrical signal is not obvious, and achieve the effect of low detection cost, simple and flexible operation, and small reagent volume
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[0029] Example 1: Preparation of aptamer sensor
[0030] (1) Prepare DNA3-GNPs: take 100 mL 0.01% HAuCl 4 Heat to boiling with stirring, then add 10 mL of 1% sodium citrate, continue heating and stirring for half an hour, then stop heating, stir and cool to room temperature to obtain gold glue. The transmission electron microscope image is shown in figure 1 in. Take 2 mL of gold gel and add 30 μL of 100 μM DNA3 solution to incubate for 12 h, then add 0.2 mL of 2 M NaCl solution, and continue to incubate for 24 h. After the completion of the reaction, the resulting solution was washed twice with water, centrifuged at 12000 r / min for 10 min, and repeated three times to prepare DNA3-GNPs. Finally, re-disperse in 600 μL Tris-HCl (pH 8.0) for use.
[0031] (2) Pretreatment of gold electrode: firstly, the gold electrode is polished with alumina powder of 1 μm, 0.5 μm, and 0.05 μm, and then polished with K 3 Fe(CN) 6 The peak potential difference for probe scanning is less than 90 mV. ...
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[0033] Example 2: Drawing the standard curve of the trace OTA standard sample by the aptamer sensor
[0034] The aptamer sensor was first placed in the OTA standard solution with the concentration of 0, 0.001, 0.01, 0.1, 0.5, 1, 2, 4 ng / mL for 15 minutes, and then rinsed with water for three times. Then immerse in 80 μM MB solution for 10 min. Second water rinsing, and then use the aptamer sensor as the working electrode, the platinum wire as the counter electrode, and the saturated calomel electrode as the reference electrode. In 50 mM Tris-HCl, DPV is used in the potential range of 0~-0.4 V Collect the corresponding MB reduction peak current signal, draw the standard curve, and display the result in image 3 in.
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