Ultra performance liquid chromatography (UPLC)-triple quadrupole/composite linear ion trap mass spectrometry detection method of triphenylmethanes and metabolites of triphenylmethanes in water bodies

Abstract

The invention belongs to the technical field of environmental monitoring and in particular relates to an ultra performance liquid chromatography (UPLC)-triple quadrupole / composite linear ion trap mass spectrometry detection method of triphenylmethanes and metabolites of triphenylmethanes in water bodies. Currently, the detection methods which can be used for simultaneously detecting five substances, including MG, LMG, CV, LCV and BG, and have detection limits up to ng level are not available yet. The method has the beneficial effects that a device adopts composite mass spectra, is an advanced novel mass spectrograph and adopts quadrupole / linear ion trap technology, thus greatly improving the MS / MS scanning capacity; meanwhile, the advanced novel mass spectrograph is connected with an UPLC, so that a simple, quick and sensitive method can be established; five triphenylmethanes and metabolites thereof in water bodies are simultaneously determined, thus providing a reliable detection method for further analyzing and evaluating the pollution conditions in the ecological environments.

Description

technical field [0001] The invention belongs to the technical field of environmental monitoring, and in particular relates to an ultra-high performance liquid chromatography-triple quadrupole / composite linear ion trap mass spectrometry detection method for triphenylmethanes and metabolites in water bodies. Background technique [0002] Malachite green (MG) and crystal violet (CV) are triphenylmethane dyes, which are widely used in aquaculture because of their disinfection and bactericidal effects. Another triphenylmethane substance, bright green (Brilliant green, BG), is often used as a triphenylmethane fuel for counterstaining cytoplasm, and there are few literature studies. It has a similar chemical structure to MG and CV, and is also easily absorbed by water. Product absorbs. The metabolic degradation products of MG and CV in vivo are Leucomalachite green (Leucomalachite green, LMG) and Leucocrystal violet (LCV), respectively. Triphenylmethanes and their metabolites hav...

AI Technical Summary

Problems solved by technology

Since LMG and LCV have no absorption peaks in the ultraviolet-visible region, and MG and CV have no fluorescence response, it is often necessary to use certain pretreatment methods when using high performance liquid phase method to simultaneously determine these substances. After PbO 2 Post-column derivatization, the recessive substances LMG and LCV are oxidized to dominant substances MG and CV, but the ratio of the derivatized column packing is affected by the mobile phase and sample matrix, and there are problems such as unstable conversion rate; while using a fluorescence detector to measure , MG and CV can be reduced to LMG and LCV by adding a reducing agent in the pretreatment process. MG, CV and their metabolic residues in water, the detection limit of this method is 0.4 μg / L

At present, there is no detection method that can simultaneously detect MG, LMG, CV, LCV, and BG with a detection limit of ng level

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