Device and method for sample treatment and particle analysis
A sample processing and microparticle technology, which is applied in the field of automation equipment, sample processing and microparticle analysis equipment, to achieve the effect of a large range of activities
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
example 1
[0116] Example 1: Five-differentiation analysis of white blood cells in a large number of whole blood samples.
[0117] That is, it is necessary to classify lymphocytes, monocytes, neutrophils, eosinophils and basophils in white blood cells, and obtain the proportion (%) and absolute count (number / liter) of various types of cells ), but no labeled reactions are required.
[0118] Experimental scheme: Self-prepared reagents are used for hemolysis and cell classification reactions. The amount of whole blood samples involved in the reaction is 35uL, the amount of reagents is 1.5mL, and the reaction time is 10 seconds.
[0119] The experiment operator sets the amount of samples and reagents involved in the reaction, the reaction time, the filling process, and the amount of each analysis on the man-machine interface of the operation control system, and the system control device automatically and accurately controls the reaction and analysis. the whole process.
[0120] The optica...
example 2
[0123] Example 2: WBC five-differential analysis of a single or small batch of whole blood samples.
[0124] Experimental scheme: Use a commercial reagent for hemolysis and cell classification reaction. The amount of whole blood sample involved in the reaction is 30uL, the amount of reagent is 1.2mL, and the reaction time is 7 seconds.
[0125] The operation method is the same as above.
[0126] Among them, the settings of the reaction parameters are different, and the optical system uses two forward scattered light detectors to capture the signal.
[0127] The samples to be tested were manually mixed and added to a 6-well microwell plate, and loaded into the labeled reaction pool. The sample aspirating needle device drives the sample aspirating needle to the position of the marked cuvette containing the sample, accurately aspirating a quantitative sample to be tested, and performing subsequent operations, as in Example 1.
example 3
[0128] Example 3: Absolute count analysis (number / liter) of Helper subclass and Suppressor subclass of T lymphocytes in a large number of whole blood samples. That is, whole blood samples need to be labeled with CD3, CD4, and CD8 monoclonal antibodies to obtain the proportion of Helper subclasses and Suppressor subclasses in white blood cells or lymphocytes; and hemolysis and cell classification reactions are required to obtain white blood cells or lymphocytes absolute concentration.
[0129] Experimental plan: A commercial antibody set A (CD3, CD4 and CD8 monoclonal antibodies are packaged together) was used for labeling incubation reaction. The amount of whole blood sample participating in the reaction was 100uL, the amount of antibody set was 25uL, and the reaction time was 10 minutes.
[0130] Self-prepared reagents were used for hemolysis and cell classification reactions. The sample volume involved in the reaction was 35 uL, the reagent volume was 1.5 mL, and the reactio...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More