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Method for removing endotoxin in lactoferrin product

A technology for lactoferrin and endotoxin, applied in the field of removing endotoxin in lactoferrin products (such as lactoferrin powder), can solve the problems of food safety risk, high cost of alkanediol, toxicity of alkanediol, etc.

Active Publication Date: 2013-12-25
INNER MONGOLIA YILI INDUSTRIAL GROUP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the method for removing endotoxins mentioned in this document is not suitable for removing endotoxins in substances such as lactoferrin that have a strong effect on endotoxins
[0007] US2011 / 0003976A1 discloses a method for removing endotoxins in protein systems by chromatography. The key technology is to use alkanediol as the eluting solvent to separate proteins and endotoxins. It is mentioned that this method is also applicable to Lactoferrin, but this method has at least two disadvantages: firstly, the cost of alkanediol is relatively high, which limits the commercialization of this method; secondly, alkanediol has certain toxicity, which poses a greater risk to food safety

Method used

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  • Method for removing endotoxin in lactoferrin product
  • Method for removing endotoxin in lactoferrin product
  • Method for removing endotoxin in lactoferrin product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Material: lactoferrin powder, endotoxin content is 104EU / mg.

[0030] Endotoxin removal process:

[0031] 1) Dissolution: Dissolve 5g of lactoferrin powder in 49L of pure water to prepare a lactoferrin solution with a mass concentration of 0.01%. Based on the mass of lactoferrin, add 10% of salt, which is composed of zinc sulfate and potassium chloride, wherein the molar ratio of zinc sulfate and potassium chloride is 100:1. Then add 5% pectin, stir gently until fully dissolved, and place at 10°C for 4 hours to fully form endotoxin polymers and endotoxin-pectin aggregates.

[0032] 2) Ultrafiltration: Select pilot-scale ultrafiltration equipment. The membrane core is a flat ultrafiltration membrane with a molecular weight cut-off of 2000kDa. Toxin lactoferrin solution, when the amount of the permeate reaches 30L, add 10L of water to the retentate, so that more lactoferrin enters the permeate through the membrane. When the permeate was about 25L, the membrane flux dec...

Embodiment 2

[0052] Material: lactoferrin powder, endotoxin content is 104EU / mg.

[0053] Endotoxin removal process:

[0054] 1) Dissolution: Dissolve 5g of lactoferrin powder in 49L of pure water to prepare a lactoferrin solution with a mass concentration of 0.01%. Based on the mass of lactoferrin, add 0.01% of salt, which is formed by chloride Calcium and ammonium sulfate, wherein the molar ratio of calcium chloride and ammonium sulfate is 1:5 (that is, the molar ratio of calcium ions to ammonium ions is 1:10). Then add 0.01% pectin, stir gently until fully dissolved, and place at 10°C for 4 hours to fully form endotoxin polymers and endotoxin-pectin aggregates.

[0055] 2) Ultrafiltration: Select pilot-scale ultrafiltration equipment. The membrane core is a flat ultrafiltration membrane with a molecular weight cut-off of 2000kDa. Toxin lactoferrin solution, when the amount of the permeate reaches 30L, add 10L of water to the retentate, so that more lactoferrin enters the permeate thro...

Embodiment 3

[0068] Material: lactoferrin powder, endotoxin content is 8.65EU / mg.

[0069] Endotoxin removal process:

[0070] 1) Dissolution: Dissolve 50kg of lactoferrin powder in 450L of pure water to prepare a lactoferrin solution with a mass concentration of 10%. Based on the mass of lactoferrin, add 0.001% of salt, which consists of magnesium chloride, Composed of sodium chloride and ammonium sulfate, the molar ratio of magnesium chloride, sodium chloride and ammonium sulfate is 1:50:25 (that is, the ratio of divalent cations to monovalent cations is 1:100). Then add 0.00005% gellan gum and 0.00005% agar, stir gently until fully dissolved, and place at 25°C for 2 hours to fully form endotoxin polymers and endotoxin-colloid aggregates.

[0071] 2) Microfiltration: select microfiltration equipment of appropriate scale, choose ceramic microfiltration membrane with a pore size of 0.1 μm as the membrane core, operate the microfiltration at 30°C, and pass the membrane pressure at 0.08MPa,...

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Abstract

The invention provides a method for removing endotoxin in a lactoferrin product. The method comprises the following steps of: preparing the lactoferrin product from which the endotoxin is to be removed into a lactoferrin aqueous solution, adding a flocculant to the lactoferrin aqueous solution so that the endotoxin therein is converted into a high polymer, and carrying out membrane separation to remove the endotoxin. The method provided by the invention also comprises the process of performing affinity chromatography on the lactoferrin aqueous solution after the membrane separation so as to further remove the endotoxin. Due to the adoption of the method provided by the invention, the removal rate of the endotoxin in the lactoferrin product can be 99.99% after the two-step separation operations of membrane separation and affinity chromatography.

Description

Technical field [0001] The invention relates to a method for removing endotoxins from lactoferrin products (such as lactoferrin powder), and belongs to the technical field of dairy processing. Background technique [0002] Lactoferrin (LF) is a glycoprotein with bioactive functions that naturally exists in exocrine fluids such as milk, tears, and saliva. Its bioactive functions include broad-spectrum antibacterial activity, enhanced iron delivery and absorption, immune regulation, Regulate inflammatory response and antioxidant effect, etc. Therefore, lactoferrin has continued to be highly praised by the market in recent years and has been widely used in food, medicine, health products, cosmetics and other fields. Commercial lactoferrin products currently used in the food industry are extracted from milk or whey. [0003] Endotoxins are the main component of the cell wall of Gram-negative bacteria. They are called endotoxins because they are released in large amounts when b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/79C07K1/36C07K1/34C07K1/30
Inventor 闫序东王彩云云战友
Owner INNER MONGOLIA YILI INDUSTRIAL GROUP CO LTD
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