Preparation method for chitosan/hydroxyapatite composite hydrogel

A technology of hydroxyapatite and composite hydrogel, which is applied in the fields of medical science and prostheses, can solve the problems of increased molecular weight polydispersity, reduced molecular weight, acid instability, etc., and achieves improved mechanical properties and tight arrangement. Effect

Inactive Publication Date: 2014-01-01
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chitosan contains glycosidic bonds of hemiacetal structure, which is unstable to acid
When the acidic solution of chitosan is placed, an acid-catalyzed hydrolysis reaction occurs, and its main chain is continuously broken due to hydrolysis, resulting in a decrease in molecular weight and an increase in molecular weight polydispersity, which in turn seriously affects the mechanical properties and other qualities of the product.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] 1) Take 8.0g chitosan, 10.0g lithium hydroxide, 1.5g sodium hydroxide, 18.0g urea, 4.0g hydroxyapatite, and 258.5g water; mix the above components and swell for 3 hours. Freeze at -60°C for 1 hour, then thaw at room temperature, filter, centrifuge and defoaming to obtain a mixed solution of chitosan / hydroxyapatite;

[0015] 2) Pour the chitosan / hydroxyapatite mixed solution into the mold, and then immerse it in a coagulation bath with a temperature of 30°C to solidify and precipitate to form a hydrogel, rinse with deionized water to pH 7.0, and obtain chitosan / Hydroxyapatite composite hydrogel, when the compression height ratio is 50%, its compressive strength is: 0.57MPa.

[0016] The coagulation bath used was 18.0 wt% H 2 SO 4 aqueous solution.

Embodiment 2

[0018] 1) Take 6.0g of chitosan, 12.0g of lithium hydroxide, 6.0g of sodium hydroxide, 18.0g of urea, 1.5g of hydroxyapatite, and 256.5g of water; mix the above components and swell for 3 hours. Freeze at -50°C for 2 hours, then thaw at room temperature, filter, centrifuge and defoaming to obtain a mixed solution of chitosan / hydroxyapatite;

[0019] 3) Pour the chitosan / hydroxyapatite mixed solution into the mold, and then immerse it in a coagulation bath with a temperature of 30°C to coagulate and precipitate to form a hydrogel, rinse with deionized water to pH 7.0, and obtain chitosan / Hydroxyapatite composite hydrogel, when the compression height ratio is 50%, its compressive strength is: 0.48MPa.

[0020] The coagulation bath used was 10.0% by mass H 2 SO 4 A mixture of aqueous solution and ethanol in a volume ratio of 1:10.

Embodiment 3

[0022] 1) Take 10.0g chitosan, 12.0g lithium hydroxide, 4.0g sodium hydroxide, 20.0g urea, 5.0g hydroxyapatite, and 249g water; mix the above components and swell for 3 hours, put the swelling solution in - Freeze at 40°C for 2.5h, then thaw at room temperature, filter, centrifuge and defoaming to obtain a mixed solution of chitosan / hydroxyapatite;

[0023] 3) Pour the chitosan / hydroxyapatite mixed solution into the mold, and then immerse it in a coagulation bath with a temperature of 30°C to coagulate and precipitate to form a hydrogel, rinse with deionized water to pH 7.0, and obtain chitosan / Hydroxyapatite composite hydrogel, when the compression height ratio is 50%, its compressive strength is: 1.06MPa.

[0024] The coagulation bath used was a mass concentration of 8.0 % H 2 SO 4 A mixture of aqueous solution and ethanol in a volume ratio of 10:1;

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Abstract

The invention discloses a preparation method for chitosan / hydroxyapatite composite hydrogel. The preparation method comprises the following steps of taking chitosan, lithium hydroxide, sodium hydroxide, urea, hydroxyapatite and water in percentage by weight; mixing the components aforementioned and then swelling, freezing, unfreezing, filtering, centrifuging and defoaming to obtain a chitosan / hydroxyapatite mixed solution; and injecting the chitosan / hydroxyapatite mixed solution in a die, then soaking in a coagulation bath, and performing coagulation and precipitation to generate the chitosan / hydroxyapatite composite hydrogel. According to the preparation method disclosed by the invention, chitosan is dissolved by a basic solvent system, thus avoiding the adverse effects of molecular weight reduction, wider distribution and the like brought by acid-catalyzed hydrolysis in the acid chitosan solution, and ensuring the existence of the macromolecular chains of chitosan in a highly-swelling state and the closer arrangement of the macromolecular chains of chitosan. In this way, hydroxyapatite is dispersed in the solution in a nanometre state, and a mesh-shaped structure formed by chitosan is used as a template to form a uniform system, thus improving the mechanical property of the chitosan / hydroxyapatite composite hydrogel.

Description

technical field [0001] The invention relates to a preparation method of a chitosan / hydroxyapatite composite hydrogel. Background technique [0002] Chitosan has the advantages of broad-spectrum antibacterial, high antibacterial efficiency and non-toxicity; at the same time, it has biodegradability, biological activity and the ability to adsorb metal ions. Clinical application proves that chitosan has the functions of promoting new skin formation, accelerating healing rate, reducing pain, etc. It can be used in medical sutures, medical non-woven fabrics, hemostatic cotton, artificial skin and tissue engineering and other fields. Chitosan hydrogel, a three-dimensional material prepared from chitosan, has excellent biocompatibility and cell affinity, and is also biodegradable. Therefore, chitosan hydrogels have extensive and in-depth applications in biomedical fields such as drug release carriers, tissue engineering scaffolds, hemostatic materials, and bone repair. On the oth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/12A61L27/52
Inventor 胡巧玲聂景怡王征科李友良
Owner ZHEJIANG UNIV
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