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Fluorescent microsphere of core shell structure and preparing method and application thereof

A technology of fluorescent microspheres and core-shell structures, which is applied to the preparation of microspheres, microcapsule preparations, chemical instruments and methods, etc., can solve the problems of troublesome preparation, few types of dyes, and low luminous intensity of fluorescent microspheres, and achieve fluorescent performance Excellent, good application prospects, good biocompatibility

Active Publication Date: 2014-01-01
INST OF CHEM MATERIAL CHINA ACADEMY OF ENG PHYSICS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fluorescent microspheres prepared by this method have a very low luminescence intensity due to the self-quenching effect of the dye molecules.
Another way is to react the dye with the monomer of silica, and then polymerize to obtain silica fluorescent microspheres, but the particle size uniformity of the silica microspheres prepared by this method is difficult to ensure, and it can Reactive dyes need to be modified in advance, and there are few types of dyes that can be grafted, and the preparation is troublesome

Method used

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  • Fluorescent microsphere of core shell structure and preparing method and application thereof
  • Fluorescent microsphere of core shell structure and preparing method and application thereof
  • Fluorescent microsphere of core shell structure and preparing method and application thereof

Examples

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Effect test

Embodiment 1

[0031]Example 1: (1) Preparation of the first polymer core: Take 100mL of purified water, add 10g of rectified styrene, 1.5g of PVP (polyvinylpyrrolidone) and 0.26g of AIBN (azobisisobutyronitrile) Put it into a 250mL three-necked flask equipped with a stirrer, a thermometer and a condenser, ultrasonically disperse for 10 minutes, stir for 1 hour under nitrogen protection, set the stirring rate at 450r / min, then slowly raise the temperature to 70°C, and continue the reaction for 12 hours. After lowering the temperature to room temperature, stop stirring, remove the nitrogen gas, centrifuge the product, wash thoroughly with ethanol, and freeze-dry to obtain the first polymer core with a particle size of 240nm and a monodispersity of less than 5%.

[0032] (2) Preparation of the second polymer core dispersed with fluorescent dyes: Take 2 grams of the prepared first polymer core, 20 mL of purified water, and 0.8 g of acrylic acid Pluronic F127 into a 50 mL single-necked flask, and...

Embodiment 3

[0041] Example 3: (1) Preparation of the first polymer core: Take 100mL of purified water, add 20g of rectified styrene, 1.5g of PVP (polyvinylpyrrolidone) and 0.20g of AIBN (azobisisobutyronitrile) Put it into a 250mL three-necked flask equipped with a stirrer, a thermometer and a condenser, ultrasonically disperse for 15 minutes, stir for 1 hour under nitrogen protection, set the stirring rate at 480r / min, then slowly raise the temperature to 70°C, and continue the reaction for 12 hours. After the temperature was lowered to room temperature, the stirring was stopped, the nitrogen was removed, the product was centrifuged and fully washed with ethanol, and freeze-dried to obtain the first polymer core with a particle size of 460nm and a monodispersity of less than 5%.

[0042] (2) Preparation of the second polymer core dispersed with fluorescent dyes: Take 4.0 g of the prepared first polymer core, 20 mL of purified water, and 0.8 g of acrylic acid Pluronic F127 into a 50 mL sin...

Embodiment 4

[0044] Example 4: (1) Preparation of the first polymer core: take 100mL of purified water, mix 10 grams of rectified methacrylate, 1.5 grams of sodium lauryl sulfate and 0.26 grams of AIBA (azobisisobutyl Add amidine hydrochloride) into a 250mL three-necked flask equipped with a stirrer, thermometer and condenser, ultrasonically disperse for 10 minutes, stir for 1 hour under nitrogen protection, set the stirring rate at 450r / min, and then slowly raise the temperature to 70°C , continue the reaction for 12 hours, lower the temperature to room temperature, stop stirring, remove the nitrogen, centrifuge the product and wash it fully with methanol, freeze-dry, and obtain the first polymer core with a particle size of 240nm, and the monodispersity is lower than 5%.

[0045] (2) Preparation of the second polymer core with fluorescent dye dispersed: take 2 grams of the prepared first polymer core, 20 mL of purified water, and 0.8 g of maleic anhydride-acrylic acid copolymer into a 50...

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Abstract

The invention discloses a fluorescent microsphere of a core shell structure. The fluorescent microsphere of the core shell structure comprises a macromolecule inner core with scattered fluorescent dye and a shell made of silicon dioxide materials, wherein the thickness of the shell is even, the diameter of the macromolecule core is 10-10000nm, and the thickness of the shell is 5-1000nm. The macromolecule core is mainly made of macromolecule materials. The fluorescent dye is one or more of perylene and derivatives of the perylene, pyrene and derivatives of the pyrene, fluorene and derivatives of the fluorene, triaryl amidogen and derivatives of the triaryl amidogen, pyrazolo quinoline and derivatives of the pyrazolo quinoline, triaryl boron and derivatives of the triaryl boron, and dye containing DCM structures. The invention further discloses a preparing method and application of the fluorescent microsphere of the core shell structure. The fluorescent microsphere of the core shell structure is even in grain size, good in biocompatibility and good in fluorescence property and is a material capable of being used for biological markers.

Description

technical field [0001] The invention relates to the technical field of organic optical functional materials, in particular to a fluorescent microsphere with a core-shell structure, a preparation method thereof, and the application of the fluorescent microsphere with a core-shell structure in biological detection, fluorescent labeling, special ink, fluid analysis and photonic crystals application. Background technique [0002] The core of fluorescence imaging, fluorescent labeling and fluorescent tracking lies in the possession of fluorescent materials with high luminous efficiency, good stability, environmental inertness, non-toxicity and convenient preparation. Traditional inorganic fluorescent compounds and quantum dots have good fluorescence stability, but generally have problems such as low extinction coefficients; while some organic / organometallic complexes have the advantages of large extinction coefficients and high fluorescence quantum efficiency, but their luminesce...

Claims

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Application Information

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IPC IPC(8): C09K11/02C09K11/06B01J13/02
Inventor 冯娇边莎黄辉蔡华强
Owner INST OF CHEM MATERIAL CHINA ACADEMY OF ENG PHYSICS
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