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Fermentation production medium and culture method of Aspergillus niger selectively synthesizing 1,3-diglyceride

A technology for the production of medium and diglycerides, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problem of low catalytic efficiency of whole-cell lipase, and achieve industrial production, mild conditions, and easy to control effects

Inactive Publication Date: 2015-11-11
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since it is an intracellular lipase, the whole-cell lipase is directly used as a catalyst, but the catalytic efficiency of this whole-cell lipase is not high (about 11%)

Method used

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  • Fermentation production medium and culture method of Aspergillus niger selectively synthesizing 1,3-diglyceride
  • Fermentation production medium and culture method of Aspergillus niger selectively synthesizing 1,3-diglyceride
  • Fermentation production medium and culture method of Aspergillus niger selectively synthesizing 1,3-diglyceride

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Experimental program
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Embodiment 1

[0023] The following are the culture medium and method involved in the present invention

[0024] (1) Seed medium: Seed medium (mass percentage): beef extract 0.3; peptone 0.5; glucose 0.5; NaCl 0.5; Sterilize at ℃ for 20min.

[0025] (2) Preparation of fermented seed liquid: Inoculate Aspergillus niger into the seed medium, culture at 30°C, 180r / min for 24 hours, and obtain the fermented seed liquid.

[0026] (3) Fermentation production medium (mass percentage): bean cake powder 2; corn steep liquor 2; K 2 HPO 4 0.5;NaNO 3 0.5; the rest is water, pH 7.0, in a 250mL Erlenmeyer flask, filled with 20%, sterilized at 121°C for 20min.

[0027] (4) Preparation of whole-cell lipase: Inoculate the fermented seed solution at 2% inoculum in the fermentation production medium for 72 hours at 30°C and 180r / min, then centrifuge at 4°C and 10,000r / min for 10min The obtained bacterium was freeze-dried and ground with liquid nitrogen protection to obtain whole-cell lipase.

[0028] (5)...

Embodiment 2

[0031] Box-Behnken design

[0032] Box-Behnken design is an experimental method suitable for optimization of 2-5 factors. Several factors screened by PB experiments that significantly affect the synthesis of lipase in Aspergillus niger CCTCCNo.M2012538 and the yield of 1,3-diglyceride were used as design factors, and the concentration obtained from the steepest climbing test was used as the center point , with the yield of 1,3-diglyceride as the response value, the medium composition was optimized by response surface analysis. See Table 1 for the experimental design, Table 2 for the experimental results, and Table 3 for the analysis of the experimental results.

[0033]

[0034] Adopt Design-Expert software to carry out variance analysis and multiple regression fitting to the data of 1,3-diglyceride yield in Table 2, obtain the significance of each influence factor (Table 3), obtain Aspergillus niger CCTCCNo.M2012538 complete The quadratic polynomial regression model equa...

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Abstract

The invention discloses a fermentation production culture medium for aspergillus niger capable of selective synthesis of 1,3-diglyceride. The medium is characterized by comprising the following components, by mass, 1.030% of glucose, 1.785% of soybean cake powder, 0.0441% of potassium chloride, 0.044% of disodium hydrogen phosphate, 0.044% of calcium chloride, 0.044% of dipotassium hydrogen phosphate and 0.044% of zinc sulfate, with the balance being water. The medium provided by the invention enables lipase to have greatly improved capability in catalysis of a glycerolysis reaction of glycerin and triglyceride and allows maximum yield of 1,3-diglyceride to reach 26.887%, increased by 2.7 times compared with yield before optimization. The proportion of 1,3-diglyceride in diglyceride is increased by 88.253%, increased by 1.26 times compared with the proportion before optimization.

Description

technical field [0001] The invention relates to a fermentation production medium and method of Aspergillus niger CCTCC NO. 2012538 for highly selective synthesis of 1,3-diglyceride. Background technique [0002] 1,3-Diglycerides are natural components of oils and are ideal for healthy fats. There are two reasons for this: First, the metabolism of 1,3-diglyceride in the human body is different from that of 1,2-diglyceride and triglyceride. Eating 1,3-diglyceride can reduce blood lipids, reduce visceral fat and effectively It can prevent obesity and various diseases mainly caused by obesity; the second is that 1,3-diglyceride is no different from triglyceride in terms of taste and flavor. Studies have shown that in recent years, 1,3-diglyceride has been widely used in food processing and as a pharmaceutical intermediate, and its research has received extensive attention. [0003] Although 1,3-diglyceride has many advantages, its content in natural oils is very low, so it is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C12R1/685
Inventor 何腊平周换景李翠芹张义明
Owner GUIZHOU UNIV
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