Method, substrate and reagents for alpha-N-acetylglucosaminidase activity detection

Abstract

The present invention provides a method for alpha-N-acetylglucosaminidase (EC3.2.1.50) activity detection, and further provides a substrate and reagents for alpha-N-acetylglucosaminidase activity determination. The method, the substrate and the reagents can be used for analyzing and determining alpha-N-acetylglucosaminidase activity in a sample (including human body fluid or tissue or cell samples) requiring determination, and are mainly used in the field of clinical laboratory. The determination reagent prepared by using the method has characteristics of convenience, rapidness and high sensitivity, and is easily promoted and applied.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and at the same time belongs to the field of clinical diagnosis and detection of genetic metabolic diseases. Specifically, the present invention provides a method for measuring the enzyme activity of α-N-acetylglucosaminidase (α-N-acetylglucosaminidase, EC 3.2.1.50), and the present invention also provides a method as α-N-acetylglucosaminidase A substrate for the detection of glucosaminidase (α-N-acetylglucosaminidase, EC 3.2. 1.50) Reagents for enzymatic activity. [0002] Background technique [0003] Lysosome is an organelle wrapped by a unit membrane in human cells. It contains a variety of acid hydrolytic enzymes. More than 60 kinds have been found so far, such as protease, nuclease, glycosidase, lipase, phosphatase, lysozyme and other enzymes. In the cell, lysosomal enzymes control the digestion of various endogenous and exogenous macromolecular substances, such as nucleic acids, pro...

AI Technical Summary

Problems solved by technology

[0009] The difficulty in diagnosing mucopolysaccharidosis type IIIB is reflected in many aspects: first, because the disease is a multi-system disease, involving genetics, nerves, muscles, blood, bones, eyes and other organs or systems, the symptoms are complex, and are related to many common diseases. There are similar clinical manifestations, so it is difficult for doctors to accumulate diagnostic experience from symptom manifestations, so missed diagnosis, misdiagnosis and delayed diagnosis are common problems

Secondly, the existing routine examinations and other auxiliary methods in the hospital are difficult to achieve the purpose of diagnosis, such as bone marrow aspiration, physical examination, electrocardiogram, electroencephalogram, B-ultrasound, CT / MRI, X-ray, muscle biopsy, serum creatine phosphokinase Many inspection methods, such as medical examination, cannot analyze the real pathogenic factors of mucopolysaccharidosis type IIIB, so it is impossible to make an accurate diagnosis of the patient's disease

Gene detection is very difficult. Due to the existence of single nucleotide sequence polymorphisms, some mutations may be normal and will not cause diseases, thus bringing uncertainty to the analysis; in addition, because the activity of the enzyme is also related to other enzymes There are many such genes that have not been discovered by researchers so far, so genetic testing cannot be used as a reliable basis for diagnosis

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