Preparation and phosphoeptide enrichment methods of titanium dioxide/ferroferric oxide nanoparticles

A technology for enriching phosphopeptides and ferric iron tetroxide, which is applied in the field of separation and purification of nanomaterials and proteins, can solve the problems of difficult enrichment of phosphopeptides and numerous steps, and achieve short enrichment period, simple operation and mild reaction conditions Effect

Inactive Publication Date: 2014-01-08
JIANGNAN UNIV
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Problems solved by technology

Enrichment of phosphopeptides by using the prepared titanium dioxide ferroferric oxide magnetic nanoparticles is easy to operate, low in cost, short in production cycle, and easy to industrialized large-scale production, which solves the problems of traditional phosphopeptide enrichment difficulties and numerous steps.

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  • Preparation and phosphoeptide enrichment methods of titanium dioxide/ferroferric oxide nanoparticles

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Embodiment 1

[0040] 1. Preparation of titanium dioxide ferric oxide magnetic nanoparticles

[0041] Synthesized by chemical co-precipitation method, the chemical reaction equation is:

[0042] Fe 2+ +2Fe 3+ +8NH 3 ·H 2 O=Fe 3 o 4 ↓+8NH 4+ +4H 2 o

[0043] Proceed as follows:

[0044] (1) the soluble Fe 2+ and Fe 3+ Dissolve in water-ethanol mixed solution (v / v=10:1), and pass nitrogen gas for 20min under mechanical stirring.

[0045] (2) Raise the temperature to 60° C., slowly drop ammonia solution into the system under stirring, adjust the pH value of the system to about 10, and obtain a black solution containing magnetic particles.

[0046] (3) Add 40 mL of butyl phthalate-ethanol mixed solution rapidly under stirring, raise the temperature to 80° C. for 1 hour, terminate the reaction, and let it stand for cooling to obtain a reaction solution.

[0047] (4) The magnetic particles are separated under the action of an external magnetic field, and washed several times with deio...

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Abstract

The lack of calcium and the low absorption efficiency of calcium become global problems, and phosphoeptide is proven to be capable of effectively promoting the calcium absorption of a human body, so that looking for efficient and fast phosphoeptide enrichment methods is of great significance. Target materials are separated and purified by taking magnetic nanoparticles as adsorption carriers is a new separation and purification technology, through carrying out surface modification on magnetic nanoparticles, target materials are specifically separated and purified, and under the action of an external magnetic field, the rapid separation of the target materials from a complex system is implemented. Compared with traditional methods, a magnetic separation technology has the advantages of simple and rapid operation, efficient enrichment, high recovery rate, and the like. The invention firstly provides a preparation method of metal oxide magnetic nanoparticles. Titanium dioxide/ferroferric oxide magnetic nanoparticles prepared by using the method disclosed by the invention have superparamagnetism and good water dispersibility, and show high specificity to the adsorption of phosphoeptide. The enrichment of phosphoeptide based on the metal oxide magnetic nanoparticles is obviously superior to that in traditional methods.

Description

technical field [0001] The invention relates to the technical field of nanomaterials and protein separation and purification, in particular to the preparation of titanium dioxide ferroferric oxide magnetic nanoparticles and a method for enriching phosphopeptides. Background technique [0002] Calcium is the most abundant trace element in the human body. It is called the "environmental guard" of the human body. It has important physiological functions, including: mildly inhibiting hyperthyroidism, preventing ectopic calcification and ossification, reducing the recurrence rate of colon cancer and Reduce the incidence of hypertension. Long-term calcium deficiency can lead to underdeveloped bones and teeth, osteoporosis, anemia, and even high blood pressure and coronary heart disease. The average daily calcium intake of Chinese residents is only about 400 mg, which only accounts for about 50% of the daily recommended amount, so calcium supplementation is a top priority. [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/06B01J20/28B01J20/30C07K1/22
Inventor 杨严俊苏宇杰汪家琦
Owner JIANGNAN UNIV
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