Ultrasonic-assisted extraction process for pleurotus eryngii polysaccharides

Abstract

The invention discloses an ultrasonic-assisted extraction process for pleurotus eryngii polysaccharides. The ultrasonic-assisted extraction process includes soaking dry pleurotus eryngii hyphae in a distilled water solvent container; placing the container in ultrasonic grinding equipment, and grinding cell wall structures of the pleurotus eryngii hyphae by the aid of ultrasonic waves to obtain pleurotus eryngii hypha mixed liquid; heating the pleurotus eryngii hypha mixed liquid by a hot water extraction method to enable polysaccharides in cells of the pleurotus eryngii hyphae to seep out so as to obtain extract liquid; performing centrifugation, concentration, ethanol precipitation and ion-exchange chromatography on the extract liquid to obtain the pleurotus eryngii polysaccharides. The ultrasonic-assisted extraction process for the pleurotus eryngii polysaccharides has the advantages that cell walls of the hyphae are broken due to extremely high pressure generated by the aid of ultrasonic cavitation, effective ingredients in the pleurotus eryngii hyphae are fed into solvents in an accelerated manner, repeated extraction is omitted, the polysaccharide yield is high, the extraction process is simplified, and the polysaccharide extraction efficiency is high.

Description

technical field [0001] The invention relates to an extraction process of Pleurotus eryngii polysaccharide, in particular to an ultrasonic-assisted extraction process of Pleurotus eryngii polysaccharide. Background technique [0002] Modern medical research has found that the physiologically active substances in edible fungi that can significantly enhance the resistance of cancer patients are edible fungus polysaccharides. Edible fungus polysaccharides have a variety of biological activities, including: anti-tumor, immune regulation, lowering blood pressure, lowering blood fat, lowering blood sugar, invigorating the stomach and protecting the liver, and anti-virus. In addition, it also has anti-oxidation, anti-aging, anti-infection, Anti-radiation, anti-edema, anti-fatigue and repair of damaged tissue cells. At present, edible fungus polysaccharides have been widely used in the clinical treatment of immunodeficiency diseases, autoimmune diseases and tumors and other uses in ...

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention and the technical task proposed are to improve the prior art, provide a kind of ultrasonic assisted Pleurotus eryngii polysaccharide extraction process, solve the traditional hot water extraction method that needs much extraction of Pleurotus eryngii polysaccharide in the current technology. Second extraction, the problem of low polysaccharide yield