Magnetic dual-template protein molecule imprinted nano particle and preparation method thereof

A nanoparticle and molecular imprinting technology is applied in the field of magnetic dual-template protein molecularly imprinted nanoparticles and their preparation, which can solve the problems of complex and changeable spatial structure, lag in imprinting research, and bulky biological macromolecules, etc. The effect of low cost and high adsorption capacity

Active Publication Date: 2014-01-15
XI AN JIAOTONG UNIV
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Problems solved by technology

However, due to the large size of biological macromolecules and the complex and changeable spatial structure, the current successful examples of molecular imprinting technology mostl

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  • Magnetic dual-template protein molecule imprinted nano particle and preparation method thereof
  • Magnetic dual-template protein molecule imprinted nano particle and preparation method thereof
  • Magnetic dual-template protein molecule imprinted nano particle and preparation method thereof

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preparation example Construction

[0037] A method for preparing magnetic double-template protein molecularly imprinted nanoparticles, comprising the following operations:

[0038] 1) Using the target protein as a template molecule, fully mix the template molecule, superparamagnetic nanoparticles functionalized with amino groups on the surface and functional monomers in the reaction buffer, and then polymerize at 20-35°C for 5-24 hours;

[0039] Wherein, the mass ratio of the template molecule to the surface amino-functionalized superparamagnetic nanoparticles is 40-80:150-300, and the mass ratio of the surface amino-functionalized superparamagnetic nanoparticles to the functional monomer is 150-300:200 ~500;

[0040]After the reaction is completed, the polymerized solid polymer is separated to obtain magnetic molecularly imprinted nanoparticles bound with template molecules;

[0041] 2) Use 0.01-0.1mol / L alkaline solution as the eluent to elute the magnetic molecularly imprinted nanoparticles combined with te...

Embodiment 1

[0053] A method for preparing magnetic double-template protein molecularly imprinted nanoparticles, comprising the following steps:

[0054] (a) Preparation of surface amino-functionalized superparamagnetic nanoparticles by hydrothermal method:

[0055] Put 5.0g of hexamethylenediamine, 3.5g of anhydrous sodium acetate, 1.5g of ferric chloride, and 30mL of ethylene glycol in a reaction kettle, stir evenly, the reaction temperature is 200°C, and the reaction time is 6h. After the reaction is completed, add The solid and liquid were separated under a magnetic field, and the solid product was washed with ultrapure water until neutral, and dried in vacuum (15Kpa, 40°C). That is to say, the surface amino functionalized superparamagnetic nanoparticles, and its electron microscope picture is as follows: figure 1 As shown, the particle size is about 60nm.

[0056] (b) Preparation of molecularly imprinted nanoparticles by immobilized template method

[0057] Take bovine serum albumi...

Embodiment 2

[0063] A method for preparing a dual-template protein molecularly imprinted polymer, comprising the steps of:

[0064] (a) Preparation of surface amino-functionalized superparamagnetic nanoparticles by hydrothermal method:

[0065] Put 3.0g of hexamethylenediamine, 2.0g of anhydrous sodium acetate, 1.0g of ferric chloride, and 20mL of ethylene glycol in a reaction kettle, and stir evenly. The reaction temperature is 190°C, and the reaction time is 8h. After the reaction is completed, add The solid and liquid were separated under a magnetic field, and the solid product was washed with ultrapure water until neutral, and dried in vacuum (15Kpa, 40°C). The surface amino-functionalized superparamagnetic nanoparticles with a particle diameter of about 65 nm were obtained.

[0066] (b) Preparation of molecularly imprinted nanoparticles by immobilized template method

[0067] Put 18.2mg BSA and 21.8mg BHb in a 100mL three-neck flask, add 20mL Tris-HCl buffer (0.1mmol / L, pH=7.0), the...

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Abstract

The invention discloses a magnetic dual-template protein molecule imprinted nano particle and a preparation method thereof. The surface amino-functionalization superparamagnetic nano particle is used as a carrier, and two types of protein are used as template molecules; the template molecules are fixed on the surface of the magnetic carrier; the polymer is formed under the effect of 3-aminopropyl triethoxysilane and octyltrimethylsilicane; then the template molecule in the polymer is washed out to obtain the magnetic dual-template protein molecule imprinted nano particle. The magnetic dual-template protein molecule imprinted nano particle prepared through the method is low in material cost, simple to prepare, rapid to separate, and various abundant protein constituents in complex biological systems are simultaneously, effectively and selectively removed and enriched more easily.

Description

technical field [0001] The invention belongs to the technical field of protein molecular imprinting, and relates to a magnetic double-template protein molecular imprinting nanoparticle and a preparation method thereof. Background technique [0002] Proteomics has become a research focus of scientists in recent years. Proteomics refers to all the proteins expressed in the genome and their way of existence. It aims to clarify the expression and function patterns of all proteins in an organism, including the identification of protein expression, existence (modified form), structure, function and interaction. function etc. Based on an important research aspect of proteomics - the study of protein expression patterns, it requires the characterization of the proteome, that is, the separation, identification and mapping of all proteins, which requires the isolation of proteins from samples and their identification . However, judging from the current technical means, there are st...

Claims

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Application Information

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IPC IPC(8): B01J20/26B01J20/28B01J20/30B01D15/08C07K1/14
Inventor 高瑞霞慕欣茹张军杰唐玉海
Owner XI AN JIAOTONG UNIV
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