Manufacturing method of low-bacterium papain

Abstract

The invention discloses a production method of low-bacteria papain, which makes full use of papaya milk, including the steps of microfiltration and ultrafiltration, and is characterized in that: in the microfiltration step, three groups of hollow fiber tubes are sequentially used to treat the papaya The milk is subjected to microfiltration: the first group is filtered with a 0.4 μm hollow fiber tube, the second group is filtered with a 0.2 μm hollow fiber tube, and the third group is filtered with a 0.1 μm hollow fiber tube; Hollow fiber combined ultrafiltration. The method reduces the number of produced papain microorganisms to less than or equal to 100, and the filtration is fast, easy to clean, and the papain does not leak, so that the production cost of the papain is reduced, and the production quality and output of the papain are improved.

Description

technical field [0001] The invention relates to a production method of protease, in particular to a production method of papain. Background technique [0002] Papaya, also known as papaya or longevity fruit, is a tropical fruit. Papaya fruit is orange-yellow or red-orange in flesh color, with high edible rate, rich in papain, vitamin C, calcium, carotene, protein and minerals, etc., with high nutritional value. The use of papain can be used in food processing industry, medicine and health products, feed additive, leather industry and textile industry, etc., and has a wide range of uses. Papain is a kind of proteolytic enzyme extracted from the milk of immature papaya fruit by means of bioengineering technology. It belongs to plant protease and has the characteristics of pure natural, safe, non-toxic and convenient to use. [0003] The existing papain extraction method is usually: raw material acceptance papaya milk→colloid mill→enzyme separation→refrigeration→microfiltrat...

AI Technical Summary

Problems solved by technology

The problems existing in this method are: (1) In the microfiltration process, the hollow fiber combined microfiltration machine with 0.2 μm hollow fiber tube has been used to filter papaya milk. It is found in practice that the hollow fiber filter tube of 0.2 μm is easy to block and cause Filtration is slow, and the filtered clear liquid has been tested, and the total number of microorganisms is ≥ 2000, which exceeds the national standard; (2) During the ultrafiltration process, a hollow fiber combined ultrafiltration machine with a molecular weight of 6000 is used to perform ultrafiltration on papaya milk. Filter, papain leaks 5%