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Oral encapsulated preparation for aquatic animals, process for the preparation of same, and usage of same

A preparation and aquaculture technology, applied in the field of preparation of aquatic product embedded preparations, can solve the problems of inability to use aquatic animals, poor vaccine stability, low vaccine viscosity, etc. Effect

Active Publication Date: 2014-01-22
维克香港贸易有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The w / o / w form of the vaccine described in the cited case has low viscosity and high fluidity, and is suitable for injection vaccines for livestock. The vaccine efficacy is similar to that of Freund's incomplete adjuvant (FIA); however , the w / o / w form of the vaccine has poor stability and is not easy to prepare. It is only used for livestock injection vaccines and cannot be used for aquatic animals and oral vaccine preparations.
[0008] It can be seen that there are still many deficiencies in the above-mentioned conventional oral aquatic product preparations, and they are not good designers, and urgently need to be improved.

Method used

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  • Oral encapsulated preparation for aquatic animals, process for the preparation of same, and usage of same
  • Oral encapsulated preparation for aquatic animals, process for the preparation of same, and usage of same
  • Oral encapsulated preparation for aquatic animals, process for the preparation of same, and usage of same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Preparation of aquatic oral vaccine

[0051] In this embodiment, vibriosis vaccines are used as embedded materials, and the aquatic oral vaccine of this embodiment is prepared in the following proportions:

[0052] A 65% by weight aqueous solution containing vibriosis vaccines;

[0053] 30% oil by weight;

[0054] 2.5% by weight of the first emulsifier;

[0055] 2.5% by weight of the second emulsifier;

[0056] First mix the oil and the first emulsifier uniformly, add 50% of the aqueous solution, shake and mix quickly at 3,000 rpm, mix the unmixed water phase substance with the second emulsifier, and add it to the above mixture , The aquatic oral vaccine of this example can be prepared by mixing with rapid shaking at 3,000 rpm;

[0057] The oil is fish oil;

[0058] Wherein the first emulsifier is sorbitol fatty acid ester, sorbitol fatty acid ester, ethylene oxide or propylene oxide concentrate;

[0059] Wherein the second emulsifier is sorbitol fatty acid ester, sorbitol...

Embodiment 2

[0061] Example 2 White shrimp seedling growth test

[0062] Experimental animal: white shrimp (Litopenaeus vannamei) shrimp seedlings with no specific pathogen free.

[0063] Test vaccine: Using the aquatic oral vaccine prepared in Example 1, a certain amount of the vaccine was diluted with 1 liter of filtered clean seawater and mixed.

[0064] Feed organisms: Artemia nauplii is used as an oral vaccine administration system.

[0065] Test group: The vaccine prepared in Example 1 was used for the test, and the diluted vaccine was fed to brine shrimp (the concentration of brine shrimp was 0.125 kg / 1 liter) for 2 hours, and then the brine shrimp was fed with white shrimp fry. From the 4th day of the juvenile stage (Post-Larva4) to the 13th day of the juvenile stage (Post-Larva13), the animals were fed three times a day, and the concentration of white shrimp larvae in the test tank was 150 per liter.

[0066] Control group: White shrimp larvae of the same batch as the experimental group bu...

Embodiment 3

[0083] Example 3 Growth test of black tiger shrimp seedlings

[0084] Test animals: black tiger prawn (Panaeus monodon) shrimp larvae with no specific pathogen (specific pathogen free).

[0085] Test vaccine: Same as Example 2.

[0086] Feed organisms: Artemia nauplii is used as an oral vaccine administration system.

[0087] Test group: The vaccine prepared in Example 1 was used for the test. The diluted vaccine was fed to brine shrimp (the concentration of brine shrimp was 0.125 kg / 1 liter) for 2 hours, and then the brine shrimp was fed to black tiger shrimp fry. From the 4th day of the juvenile stage (Post-Larva4) to the 13th day of the juvenile stage (Post-Larva13), the black tiger prawns were fed three times a day, and the concentration of black tiger prawns in the test tank was 150 per liter.

[0088] Control group: Black tiger shrimp larvae from the same batch as the test group but not administered with the vaccine.

[0089] Both the test group and the control group were tested i...

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Abstract

The invention provides an oral encapsulated preparation for aquatic animals in the form of w / o / w emulsion which comprises on a weight basis: 40-80% of an aqueous phase containing water soluble active substances; 18-50% of oily phase comprising one or more oils; 1-5% of the first emulsifiers; 1-5% of the second emulsifiers. The preparation method comprises mixing uniformly the oily phase with the first emulsifiers; adding the aqueous phase to the mixture and mixing them by highspeed concussion to form water in oil emulsion; mixing uniformly the remaining aqueous phase with the second emulsifiers, then adding the mixture to the water in oil emulsion and mixing by highspeed concussion to form w / o / w emulsion.

Description

Technical field [0001] The present invention relates to an oral aquatic product embedding preparation, in particular to a small particle, high stability, good palatability, can be used as aquatic oral vaccine and various supplements (nutrients, immune enhancers or accelerators , Pathogen elimination preparations, etc.) oral aquatic embedded preparations; the present invention further relates to the preparation and use methods of the aquatic embedded preparations. Background technique [0002] China’s aquaculture industry is technologically advanced and ranks among the world’s three largest aquaculture kingdoms with Japan and Norway. However, Taiwan’s location in the subtropical zone and high aquaculture density makes it easy to become a breeding ground for aquatic diseases, and densely colonized aquatic animals require nutrients and breeding environment Special attention must be paid, otherwise it will easily cause high mortality of aquatic animals and have a huge impact on the e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/113A61K39/106A61K47/44A23K1/16A61P31/04
CPCA23K40/30A23K20/158A23K50/80Y02A40/818A61P31/04
Inventor 郭树勇陈旭中
Owner 维克香港贸易有限公司