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Modified cellulose nanofiber membrane based on layer-by-layer self-assembly of lysozyme and silk protein based as well as preparation and application thereof

A technology of nanofiber membrane and layer-by-layer self-assembly, applied in the fields of polymer materials and biomedical materials, can solve the problem of no biological application, and achieve the effects of low cost of raw materials, simple preparation equipment, and simple operation.

Active Publication Date: 2015-07-15
HUBEI SAILUO BIOLOGICAL MATERIAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patent with the application number "CN201010190222.6" reported the preparation method of nanofiber immobilized β-D-galactosidase, but did not conduct research on biological application

Method used

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  • Modified cellulose nanofiber membrane based on layer-by-layer self-assembly of lysozyme and silk protein based as well as preparation and application thereof
  • Modified cellulose nanofiber membrane based on layer-by-layer self-assembly of lysozyme and silk protein based as well as preparation and application thereof
  • Modified cellulose nanofiber membrane based on layer-by-layer self-assembly of lysozyme and silk protein based as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Prepare cellulose acetate nanofiber membrane by electrospinning technology, that is, dissolve cellulose acetate in acetone / dimethylacetamide (mass ratio 2:1) to prepare 25% (w / w) cellulose acetate solution, the electrospinning voltage was 35kV, the flow rate was 0.5mL / h, the temperature was 5°C, the relative humidity was 20%, and the duration was 96h; then the obtained cellulose nanofiber membrane was hydrolyzed in 0.5M NaOH solution for 7 days.

[0030] (2) At room temperature, soak the prepared cellulose nanofiber membrane in 0.2mg / L lysozyme solution for 5 minutes, then wash it three times with 0.2mg / L NaCl solution, and then wash it in 0.2mg / L silk protein solution Soak for 5 minutes, then wash three times with 0.2mg / L NaCl solution, and assemble in the same way (lysozyme / silk protein) n (n = 30) bilayers to obtain a layer-by-layer self-assembled modified cellulose nanofiber membrane based on lysozyme and silk protein.

Embodiment 2

[0032] (1) Prepare cellulose acetate nanofiber membrane by electrospinning technology, that is, dissolve cellulose acetate in acetone / dimethylacetamide (mass ratio 2:1) to prepare 20% (w / w) cellulose acetate solution, the electrospinning voltage was 20kV, the flow rate was 1mL / h, the temperature was 35°C, the relative humidity was 80%, and the duration was 36h; then the obtained cellulose nanofiber membrane was hydrolyzed in 0.2M NaOH solution for 6 days.

[0033] (2) At room temperature, soak the prepared cellulose nanofiber membrane in 0.1mg / L lysozyme solution for 25 minutes, then wash it three times with 0.1mg / L NaCl solution, and then wash it in 0.1mg / L silk protein solution Soak for 25 minutes, then wash three times with 0.1mg / L NaCl solution, and assemble in the same way (lysozyme / silk protein) n (n=50.5) bimolecular layers to obtain a layer-by-layer self-assembled modified cellulose nanofiber membrane based on lysozyme and silk protein.

Embodiment 3

[0035] (1) Cellulose acetate nanofiber membrane was prepared by electrospinning technology, that is, cellulose acetate was dissolved in acetone / dimethylacetamide (mass ratio 2:1) to prepare 16% (w / w) cellulose acetate solution, the electrospinning voltage was 15kV, the flow rate was 1mL / h, the temperature was 25°C, the relative humidity was 45%, and the duration was 24h; then the obtained cellulose nanofiber membrane was hydrolyzed in 0.1M NaOH solution for 7 days.

[0036] (2) At room temperature, soak the prepared cellulose nanofiber membrane in 0.1mg / L lysozyme solution for 20 minutes, then wash it three times with 0.1mg / L NaCl solution, and then wash it in 0.1mg / L silk protein solution Soak in water for 20 minutes, then wash three times with 0.1mg / L NaCl solution, and assemble in the same way (lysozyme / silk protein) n (n = 5) bilayers to obtain a layer-by-layer self-assembled modified cellulose nanofiber membrane based on lysozyme and silk protein. The morphology of the m...

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Abstract

The invention discloses a modified cellulose nanofiber membrane based on layer-by-layer self-assembly of lysozyme and silk protein as well as preparation and application thereof and belongs to the technical fields of high polymer materials and biomedical materials. According to the invention, a cellulose acetate nanofiber membrane is prepared by utilizing an electrospinning technique and lysozyme and silk protein which are opposite in charges are alternately assembled on the surface of the cellulose nanofiber membrane by adopting a layer-by-layer assembly technique so as to obtain the modified cellulose nanofiber membrane disclosed by the invention. The membrane disclosed by the invention has the advantages that the preparation equipment is simple, raw materials are low in cost, non-toxic and biodegradable, the whole process is simple to operate and the number of lysozyme / silk protein layers is controllable. Proved by antibacterial tests, cell survival tests, cell fluorescence staining tests, cell adhesion tests and in vitro wound healing tests, the modified cellulose nanofiber membrane disclosed by the invention has good antibacterial performance and cell adhesiveness and is capable of increasing the wound healing efficiency and preventing the wound infection, so that the membrane is a very good wound repairing material and can be used in the wound repairing field.

Description

technical field [0001] The invention belongs to the technical field of polymer materials and biomedical materials, and relates to a layer-by-layer self-assembled modified cellulose nanofiber membrane based on lysozyme and silk protein and its preparation and application. Background technique [0002] Due to its excellent biocompatibility, silk protein has been widely studied in the field of tissue engineering. At the same time, it has excellent compatibility with human dermis and can play an important role in wound repair. Lysozyme is a natural anti-infective substance with bactericidal effect. It has antibacterial, antiviral, hemostatic, swelling and pain relief, and accelerated tissue function recovery. However, industrial production of lysozyme often encounters poor stability and is easily affected by the external environment. Problems such as easy inactivation and difficulty in recycling limit its application in the fields of biomedical materials, drug controlled releas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L15/38A61L15/32A61L15/28A61L15/44A61L15/46
Inventor 邓红兵王先恺詹颖菲忻尚璟杜予民施晓文
Owner HUBEI SAILUO BIOLOGICAL MATERIAL CO LTD
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