Method for preparing Soraean gum and application thereof in water treatment

A solar glue and water treatment technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, flocculation/sedimentation water/sewage treatment, etc., can solve the problems of refractory secondary pollution, strong neurotoxicity, etc., and achieve Low cost, clear ingredients, fast flocculation effect

Inactive Publication Date: 2014-01-29
NANJING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Polyacrylamide itself is not toxic, but its refractory property easily causes secondary pollution, and studies have shown that acrylamide monomer is a strong carcinogen with strong neurotoxicity (H. Yokoi, T. Obita, J. Hirose, S. Hayashi, Y. Takasaki. Flocculation properties of pectin in various suspensions. Bioresource Technology. 84:287-290.)

Method used

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  • Method for preparing Soraean gum and application thereof in water treatment
  • Method for preparing Soraean gum and application thereof in water treatment
  • Method for preparing Soraean gum and application thereof in water treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Such as figure 1 As shown, prepare the culture medium.

[0026] Seed culture solution: NaH 2 PO 4 0.25g, KNO 3 1.5g, CaCl 2 0.05g, MgCl 2 0.15g, FeSO 4 ·7H 2 O 0.004g, MnSO 4 0.001g, ZnCl 2 0.004g, sucrose 20g, H 2 O 1000 mL, pH7.2, fermentation culture solution Add 30g NaCl to the seed culture solution, heat the seed culture solution at 110°C for 30 minutes to sterilize, add 100 μL plate colony liquid to the seed culture solution cooled to 30°C, and incubate at 28°C Ferment for 18 hours to form seed liquid, wash the brewer's grains twice with water, add 15mL of fermentation culture liquid, and then add 5% seed liquid, ferment at 28 ℃ for 48h, add 20mL of distilled water to the fermentation bottle to wash the brewer's grains twice, and collect supernatant, inject more than twice the volume of ethanol into the hair supernatant, and vacuum-dry at 50°C to obtain the crude product of bioflocculant sola gum.

[0027] Detection of flocculation effect: take 1...

Embodiment 2

[0033] Such as figure 2 As shown, prepare the culture medium.

[0034] Seed culture solution: NaH 2 PO 4 0.5g, KNO 3 2.5g, CaCl 2 0.07g, MgCl 2 0.2g, FeSO 4 ·7H 2 O 0.006g, MnSO 4 0.002g, ZnCl 2 0.006g, sucrose 40g, H 2 O 1000 mL, pH8.0, fermentation culture solution Add 30g NaCl to the seed culture solution, heat the seed culture solution at 110°C for 30 minutes to sterilize, add 100 μL plate colony liquid to the seed culture solution cooled to 32°C, and incubate at 28°C Ferment for 20 hours to form seed liquid, wash the brewer's grains twice with water, add 15mL of fermentation culture liquid, then add 8% seed liquid, ferment at 28 ℃ for 48h, add 20mL of distilled water to the fermentation bottle to wash the brewer's grains twice, collect supernatant, inject more than twice the volume of isopropanol into the hair supernatant, and vacuum-dry at 50°C to obtain the crude product of bioflocculant Sola gum.

[0035] Detection of flocculation effect: Take 1000mL ...

Embodiment 3

[0038] Such as figure 1 As shown, prepare the culture medium.

[0039] Seed culture solution: NaH 2 PO 4 2g, KNO 3 3g, CaCl 2 0.07g, MgCl 2 0.25g, FeSO 4 ·7H 2 O 0.01g, MnSO 4 0.002g, ZnCl 2 0.008g, 30g sucrose, H 2 O 1000 mL, pH 6.0, fermentation culture solution Add 40g NaCl to the seed culture solution, heat the seed culture solution at 121°C to sterilize for 15 minutes, add 150 μL plate colony liquid to the seed culture solution cooled to 37°C, and incubate at 28°C Ferment for 20 hours to form seed liquid, wash the brewer's grains twice with water, add 20mL of fermentation culture liquid, and then add 8% seed liquid, ferment at 28 ℃ for 56h, add 20mL of distilled water to the fermentation bottle to wash the brewer's grains twice, and collect supernatant, inject more than twice the volume of acetone into the hair supernatant, and vacuum-dry at 50°C to obtain the crude bioflocculant sola gum.

[0040] Flocculation treatment of Yangtze River water in Nanjing...

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Abstract

The invention discloses methods for producing water-soluble bioflocculant Soraean gum through solid-state fermentation of Agrobacterium ZX09 by using an inorganic-salt culture solution and treating high-turbidity and eutrophicated river / lake water. According to the methods, Agrobacterium is subjected to solid-state fermentation in the culture solution prepared from inorganic salts so as to obtain a viscous metabolic product, ethanol of a volume which is twice that of fermentation liquor is added into the fermentation liquor for precipitation, and a precipitate is subjected to vacuum drying, so that a crude product of bioflocculant Soraean gum can be obtained. A Soraean gum solution is prepared, kaolin, diatomite, activated carbon, yeast particles and suspension particles are put into wastewater, the crude product of Soraean gum and polymeric ferric sulfate are used in a compounded manner so as to treat the high-turbidity and eutrophicated river / lake water, and then, the particle content and COD (Chemical Oxygen Demand) content of the river / lake water can be lowered effectively; the flocculation activity is high, the usage amount is small, the flocculation rate is high, and the pH range applicable to treatment is wide, so that environmental conservation and large-scale industrial production are facilitated.

Description

technical field [0001] The present invention relates to Agrobacterium Agrobacterium The preparation method of sp. ZX09 sola gum solid-state fermentation and its application in water treatment. Background technique [0002] At present, my country's water pollution has extended from surface water pollution to groundwater pollution, and from conventional pollution to toxic chemical pollution. The situation is severe and has become a restrictive factor restricting the stable development of my country's economy and society. Flocculation is a common means and method for water purification in water treatment, especially the pretreatment of various sewage treatment. The commonly used traditional flocculants are polyaluminum trichloride and polyacrylamide. As an inorganic polymer flocculant, polyaluminum trichloride has strong charge neutralization and sticky bridging effects, but the flocculation and sedimentation speed is slow and the residence time is long, and Al 3+ It is toxi...

Claims

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Application Information

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IPC IPC(8): C12P19/04C02F1/56C12R1/01
Inventor 张建法朱斌周梦怡
Owner NANJING UNIV OF SCI & TECH
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