Preparation method and use of active low molecular weight fucosan

A fucoidan, low molecular weight technology, applied in application, food science, food preservation, etc., can solve the problems of high molecular weight and lack of physiological activity of polysaccharides, and achieve the effects of uniform molecular weight, strong controllability and high yield

Active Publication Date: 2014-02-05
厦门百拓生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The existing technology only separates fucoidan from seaweed. At this time, the polysaccharide has a large molecular weight and lacks physiological activity.

Method used

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  • Preparation method and use of active low molecular weight fucosan
  • Preparation method and use of active low molecular weight fucosan
  • Preparation method and use of active low molecular weight fucosan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: the preparation of fucoidan

[0033] 80-mesh kelp powder was added to deionized water at a ratio of 1:25 (w / v), extracted at 80 °C for 3 h, stirred continuously during the extraction process, the stirring speed was 2000 r / min, and centrifuged (3000 r / min, 10 min) , take the supernatant, add HCl (12 mol / L) to make the final concentration 0.1 mol / L; then centrifuge (3000 r / min, 5 min), remove the alginic acid precipitate, collect the supernatant, add absolute ethanol Make the final concentration 30% (v / v), overnight; centrifuge (5000 r / min, 10 min), take the supernatant, and add absolute ethanol to make the final concentration 60% (v / v), Overnight; centrifuge (3000 r / min, 5 min), take the precipitate, and dry it in vacuum to obtain dry fucoidan powder.

[0034] The obtained fucoidan is subjected to infrared spectroscopic analysis, and the results are shown in figure 1 with figure 2 , figure 1 Medium 846 cm -1 The absorption peak at shows that the p...

Embodiment 2

[0035] Embodiment 2: Preparation of low molecular weight fucoidan

[0036] Hydrolysis: Mix the fucoidan dry powder obtained in Example 1 with β-galactosidase (Shanghai Jiahe Biotechnology Co., Ltd., model: Amresco9031-11-2) and 30000 U / g enzyme activity respectively. g of β-glucosidase (Shanghai Hualan Chemical Technology Co., Ltd., model: 9001-22-3) was mixed evenly at a weight ratio of 1000:0.5:0.5, and deionized water was added to make 3% by weight (w / w) sugar solution, add 4.0 mol / L HCl to adjust the pH value to 5.0, hydrolyze at 37 °C for 5 h, then add 4 mol / L NaOH aqueous solution to adjust the pH value to neutral, heat to 80 °C and keep it warm for 5 min , thereby terminating the hydrolysis reaction, and obtaining low-molecular-weight fucoidan aqueous solutions of different molecular fragments.

[0037] Separation: 100 L of low-molecular-weight fucoidan aqueous solution containing different molecular fragments was separated by a medium-sized ultrafiltration membrane...

Embodiment 3

[0039] Embodiment 3: Antibacterial experiment of low-weight fucoidan

[0040] Staphylococcus aureus ( Staphyloccocus aureus ) strain (the strain number is ACCCNO.02863, and the preservation date is October 20, 2007) and Escherichia coli ( Escherichia coli ) strain (the strain number is ACCCNO.01623, and the preservation date is May 22, 2006) provided by the China Agricultural Microorganism Culture Collection Management Center.

[0041] 1. Experimental method

[0042] a. According to the method of Example 1, extract fucoidan from kelp; according to the method of Example 2, respectively prepare fucoidan with a molecular weight range of ≤5000 Da, 5000-100000 Da and ≥100000 Da, barium sulfate-suspended Nephelometric method detects that sulfate radical content is respectively 28.2 mass %, 26.9 mass % and 25.3 mass %;

[0043] b. Prepare a polysaccharide mother liquor with a concentration of 3.0% by weight of the low-molecular-weight fucoidan prepared in step a, filter an...

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Abstract

The invention discloses a preparation method and use of active low molecular weight fucosan. The method comprises the following steps: hydrolyzing the fucosan by using beta-galactosidase and beta-glucosaccharase to obtain an oligomerized fucosan aqueous solution with different molecular fragments; separating through an ultrafiltration membrane to obtain the low molecular weight fucosan at 5000-100000 Da. The preparation method of enzymatically preparing the low molecular weight fucosan is mild in reaction condition, strong in controllability, and high in retention rate of sulfate radicals which are the key active groups of the low molecular weight fucosan; the obtained low molecular weight fucosan has a good antibacterial effect and antibacterial stability. The prepared low molecular weight fucosan is a safe and environment-friendly product applied to antibacteria, and can be used for food fresh keeping and storage, and the use of pesticides and antibiotics is reduced so as to improve the security of the food.

Description

Technical field [0001] The invention is the field of biological preparations, especially the preparation method of active low molecular quantum alcoholic glycogen and its application in antibacterial. Background technique [0002] "People are used as the sky". With the rapid development of the global economy, people have changed fundamentally in terms of eating.There will be more consumers in food consumption not only satisfied in quantity, but also higher requirements in the quality of food.The abuse of pesticides has hindered the healthy development of modern agriculture, and the dining table pollution has also become a serious social issue that endangers the health of the people.No pollution, pollution -free, and high -quality green food will gradually become popular food.In addition, the widespread use of antibiotics has led to a large number of clinical resistance.According to reports, the current tolerance rate of germine to penicillin has reached more than 70 %, and the to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A23L3/3562
Inventor 吴永沛刘翼翔
Owner 厦门百拓生物工程有限公司
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