A kind of preparation method and application of layer-by-layer self-assembled microrna nano-carrier

A layer-by-layer self-assembly and nano-carrier technology is applied in the field of nanomaterial biology to achieve the effects of simple preparation method, high repeatability and good stability

Inactive Publication Date: 2016-07-13
成都达普医疗器械有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

We use nanoparticles formed by the interaction of nanodiamonds and protamine sulfate as siRNA carriers (patent publication number: 103060379A), but so far, there have been no reports of using such particles to adsorb miRNA and have cancer cell targeting effects

Method used

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  • A kind of preparation method and application of layer-by-layer self-assembled microrna nano-carrier
  • A kind of preparation method and application of layer-by-layer self-assembled microrna nano-carrier
  • A kind of preparation method and application of layer-by-layer self-assembled microrna nano-carrier

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Experimental program
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Embodiment

[0031] 1. Preparation of nanodiamonds

[0032] Dilute the nanodiamond (Nanodiamond, ND, hereinafter referred to as ND) hydrogel (catalogue number: 386-8567, Tokida, Ueda, Nagano) with a concentration of 150 mg / ml with deionized water into an aqueous solution with an ND concentration of 1 mg / ml, Take 2ml and use a KQ-100DB ultrasonic cleaning machine (Kunshan Ultrasonic Instrument Co., Ltd.) to sonicate for 2h at a power of 100W to obtain an aqueous solution with an ND concentration of 1mg / ml.

[0033] 2. Nano-diamond surface oxidation

[0034] Mix nitric acid with a mass fraction of 98% and sulfuric acid with a mass fraction of 98% at a mass ratio of nitric acid: sulfuric acid = 3:1, and place the aqueous solution with an ND concentration of 1 mg / ml obtained in 1 at 140°C in the above nitric acid and sulfuric acid React in the mixed solution for 12 hours, centrifuge the reaction product, wash the obtained precipitate with deionized water and centrifuge, repeat 3 times, finall...

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Abstract

A preparation method and application of a layer-by-layer self-assembled microRNA nanocarrier belong to the field of nanomaterial biology. The invention relates to a layer-by-layer self-assembly technology based on electrostatic attraction, which uses nano-diamond as the base material, protamine sulfate with good biological safety and small molecule folic acid with tumor targeting function as the self-assembly material. A microRNA nanocarrier with folate receptor targeting function was constructed. The microRNA nano-carrier prepared by the invention has the characteristics of self-assembly, strong microRNA protection ability, strong cell targeting ability, high gene silencing efficiency and the like.

Description

Technical field: [0001] The invention belongs to the field of nanometer material biology. The invention relates to a layer-by-layer self-assembly technology based on electrostatic attraction, which uses nano-diamond as the base material, protamine sulfate with good biological safety and small molecule folic acid with tumor targeting function as the self-assembly material. A microRNA nanocarrier with folate receptor targeting function was constructed. The microRNA nano-carrier prepared by the invention has the characteristics of self-assembly, strong microRNA protection ability, strong cell targeting ability, high gene silencing efficiency and the like. Background technique: [0002] RNA interference (RNAi) is a natural mechanism of gene silencing present in plant and mammalian cells. microRNA is a kind of endogenous small RNA with RNA interference activity that people have just discovered in recent years. Such small RNAs are generally called small interfering RNAs. When ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/87A61K48/00A61K47/42A61K47/04A61K9/14
Inventor 盛望邓雄威曹敏军曾毅肖向茜胡琴张芳
Owner 成都达普医疗器械有限公司
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