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Immunopeptide freeze-dried powder and preparation method thereof

A technology of peptide freeze-dried powder and immune activity, which is applied in the field of immune-active peptide freeze-dried powder and its preparation, can solve the problem of enhancing the functionality of peptide products, which has not yet been explored, and achieves maintaining physical and chemical properties, improving proliferation ability, and shortening enzymes. The effect of solution time

Active Publication Date: 2014-02-05
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, researchers have not yet explored the use of PEF to improve the efficiency of enzymatic hydrolysis and enhance the functionality of peptide products.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: The corn gluten powder with a moisture content of 3.5% and a protein content of 66% was ultrafinely pulverized to a particle size of 320 mesh, and the corn gluten ultrafine powder was obtained and sealed for later use. Use purified water to adjust the 320-mesh zein superfine powder to a zein solution with a substrate protein concentration of 2% (W / W). The zein solution was homogenized by using a high-speed dispersing homogenizer, the control speed was 2000r / min, and the processing time was 10min. Under the condition of an ambient temperature of 25 °C, a double electrode with a length of 1 mm and a radius of 0.5 mm was installed on a high-voltage pulsed electric field device, and 75% ethanol and distilled water were used to clean the material circulation pipeline in the device twice respectively. Set the electric field strength of the device to 10kV / cm, and the electric field frequency to 2000Hz, and then stand by; then use 1M NaOH solution to adjust the pH va...

Embodiment 2

[0034] Example 2: Wheat protein powder with a moisture content of 4.5% and a protein content of 69% was ultrafinely pulverized to a particle size of 320 mesh, and the wheat protein ultrafine powder was sealed for later use. Use purified water to adjust the 320-mesh wheat protein superfine powder to a wheat protein solution with a substrate protein concentration of 4% (W / W). The wheat protein solution was homogenized by using a high-speed dispersing homogenizer, the control speed was 2000r / min, and the processing time was 10min. Under the condition of an ambient temperature of 25 °C, a double electrode with a length of 1 mm and a radius of 0.5 mm was installed on a high-voltage pulsed electric field device, and 75% ethanol and distilled water were used to clean the material circulation pipeline in the device twice respectively. Set the electric field strength of the device to 10kV / cm, and the electric field frequency to 1000Hz in the working state, and then stand by; then use 1...

Embodiment 3

[0035]Example 3: Soybean protein powder with a moisture content of 4.7% and a protein content of 43% was ultrafinely pulverized to a particle size of 320 mesh, and the soybean protein ultrafine powder was sealed for later use. Use purified water to adjust the 320-mesh soybean protein superfine powder to a soybean protein solution with a substrate protein concentration of 1% (W / W). The soybean protein solution was homogenized by using a high-speed dispersing homogenizer, the control speed was 4000r / min, and the processing time was 10min. Under the condition of an ambient temperature of 25°C, a double electrode with a length of 3 mm and a radius of 1 mm was installed on a high-voltage pulsed electric field device, and 75% ethanol and distilled water were used to clean the material circulation pipeline in the device twice respectively. The electric field strength of the device is 40kV / cm, the electric field frequency is 3000Hz, and it is on standby; then use 1M NaOH solution to a...

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Abstract

The invention discloses immunopeptide freeze-dried powder and a preparation method thereof, belongs to the technical fields of deep-processing of agricultural products and comprehensive utilization of by-products thereof, and relates to immunopeptide freeze-dried powder and a preparation method thereof. With any one of corn gluten meal, wheat protein powder, rice protein powder, soyabean protein powder, mung bean protein powder and poultry and egg white powder as a raw material, a process route comprising superfine grinding of designed raw material, blending and homogenizing of a protein solution, controlled enzymatic hydrolysis under an environment of a high-voltage pulsed electric field, low-temperature enzyme deactivation, low-temperature centrifugation, low-temperature membrane separation and vacuum freeze drying is realized, wherein the moisture content of the raw material is 1.0-5.0% and the protein content of the raw material is 40-70%; the immunopeptide freeze-dried powder of which the moisture content is 1.2-1.8%, the protein content is 72-86% and the molecular weight is 10-30 kDa is obtained. Compared with a control group, an immune activity evaluation experiment proves that the multiplication capacity of splenic lymphocyte of mice of an experimented group is significantly improved.

Description

technical field [0001] The invention belongs to the technical field of intensive processing of agricultural products and the comprehensive utilization of by-products thereof, and relates to a freeze-dried immune active peptide powder and a preparation method thereof, which can realize the use of high-voltage pulsed electric field technology to obtain a product with a moisture content of 1.2 under normal temperature conditions. % to 1.8%, protein content of 72% to 86%, and molecular weight of 10 to 30kDa immunoactive peptide freeze-dried powder, the immune activity evaluation experiment proved that compared with the control group, the spleen lymphocytes of the mice in the test group proliferated The ability was significantly improved by 10% to 20% (P<0.01). Background technique [0002] In recent years, with the deepening understanding of various immune mechanisms and regulatory pathways, immunoactive peptides have increasingly become a research hotspot in the field of bio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/415C07K14/465C07K1/34
Inventor 林松毅王可刘静波
Owner JILIN UNIV
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