Immunopeptide freeze-dried powder and preparation method thereof

Abstract

The invention discloses immunopeptide freeze-dried powder and a preparation method thereof, belongs to the technical fields of deep-processing of agricultural products and comprehensive utilization of by-products thereof, and relates to immunopeptide freeze-dried powder and a preparation method thereof. With any one of corn gluten meal, wheat protein powder, rice protein powder, soyabean protein powder, mung bean protein powder and poultry and egg white powder as a raw material, a process route comprising superfine grinding of designed raw material, blending and homogenizing of a protein solution, controlled enzymatic hydrolysis under an environment of a high-voltage pulsed electric field, low-temperature enzyme deactivation, low-temperature centrifugation, low-temperature membrane separation and vacuum freeze drying is realized, wherein the moisture content of the raw material is 1.0-5.0% and the protein content of the raw material is 40-70%; the immunopeptide freeze-dried powder of which the moisture content is 1.2-1.8%, the protein content is 72-86% and the molecular weight is 10-30 kDa is obtained. Compared with a control group, an immune activity evaluation experiment proves that the multiplication capacity of splenic lymphocyte of mice of an experimented group is significantly improved.

Description

technical field

[0001] The invention belongs to the technical field of intensive processing of agricultural products and the comprehensive utilization of by-products thereof, and relates to a freeze-dried immune active peptide powder and a preparation method thereof, which can realize the use of high-voltage pulsed electric field technology to obtain a product with a moisture content of 1.2 under normal temperature conditions. % to 1.8%, protein content of 72% to 86%, and molecular weight of 10 to 30kDa immunoactive peptide freeze-dried powder, the immune activity evaluation experiment proved that compared with the control group, the spleen lymphocytes of the mice in the test group proliferated The ability was significantly improved by 10% to 20% (P<0.01). Background technique

[0002] In recent years, with the deepening understanding of various immune mechanisms and regulatory pathways, immunoactive peptides have increasingly become a research hotspot in the field of bio...