The invention provides a cargo compartment door opening and closing positioning device which comprises a left compartment door, a right compartment door and a compartment body, and the left compartment door and the right compartment door are both rotationally arranged on the rear portion of the compartment body and each comprise a first positioning assembly, a clamping table and a mounting plate;two groups of first positioning assemblies are arranged; the first positioning assemblies comprise first positioning rods, second positioning rods, rocking handles, gears, first racks, first connecting plates, first elastic pieces, second racks, second connecting plates, second elastic pieces, first mounting covers and positioning tables used for penetrating through the first positioning rods andthe second positioning rods; the mounting plate is of an I-shaped structure; the number of the clamping tables is four, the four clamping tables are located at the four ends of the mounting plate respectively, and clamping grooves used for clamping the first connecting plates and the second connecting plates are formed in the clamping tables; and a second positioning assembly for driving the mounting plate to move is arranged on the left compartment door or the right compartment door. The two compartment doors can be positioned, the closing firmness of the two compartment doors is improved, and the driving safety is guaranteed.