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Marine microorganism adhesion sensor based on organic electrochemical transistor and preparation method of sensor

A technology of marine microorganisms and transistors, applied in the field of biosensors, can solve problems such as equipment stability and safety impact, loss of working ability, and microbial fouling of marine engineering equipment, achieving low cost, simple structure, and easy on-site long-term monitoring. Effect

Inactive Publication Date: 2014-02-05
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the marine environment, microorganisms such as bacteria and diatoms usually quickly attach to the surface of the material and form a microbial film, causing microbial fouling of marine engineering equipment, greatly affecting the stability and safety of the equipment, and even losing work ability

Method used

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  • Marine microorganism adhesion sensor based on organic electrochemical transistor and preparation method of sensor
  • Marine microorganism adhesion sensor based on organic electrochemical transistor and preparation method of sensor

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Effect test

Embodiment 1

[0039] A marine microbial sensor based on an organic electrochemical transistor consists of a substrate 1, a drain electrode 2 and a source electrode 3, a conductive polymer layer 4, a water storage tank 5, and a gate electrode 6, and the drain electrode 2 and source electrode 3 are located between the substrate 1 Above, the conductive polymer layer 4 connects the drain electrode 2 and the source electrode 3, the water storage tank 5 is located on the conductive polymer layer 4, the gate electrode 6 is located in the water storage tank 5, suspended above the conductive polymer layer 4, through the electrolyte and The conductive polymer layer 4 is connected. The conductive polymer layer is made of conductive polymer poly(3,4-dioxyethylthiophene) / polystyrene sulfonic acid (PEDOT:PSS).

[0040] The glass substrate was cleaned by ultrasonic cleaning with deionized water, acetone and absolute ethanol for 10 min respectively. After cleaning, put the glass substrate into a UV surfac...

Embodiment 2

[0043] The PET polyester film was cleaned by ultrasonic cleaning with deionized water, acetone and absolute ethanol for 10 min respectively. After cleaning, put it into a UV surface irradiation cleaning machine for cleaning for 10 minutes; use a vacuum evaporation method to evaporate the source electrode and the drain electrode, the source and drain electrodes are Cu electrodes, the evaporation rate is 0.2 nanometers per second, and the thickness is 10 nanometers; Spin-coat the conductive polymer layer polypyrrole (PPy) at a speed of 2000 rpm with a thickness of 5nm; then anneal at 200°C for 1 hour under a nitrogen atmosphere; then fix the silicone tube on the surface of the device with glue and seal it into a water storage pool ; Finally, the silver / silver chloride electrode is inserted into the water storage tank as a grid; the organic electrochemical transistor marine microbial sensor is obtained.

Embodiment 3

[0045]The silicon wafer substrate was cleaned by ultrasonic cleaning with deionized water, acetone, and absolute ethanol for 10 min respectively. After cleaning, put it into a UV surface irradiation cleaning machine for cleaning for 10 minutes; use the vacuum evaporation method to evaporate the source electrode and the drain electrode, the source and drain electrodes are both indium tin oxide (ITO) electrodes, and the evaporation rate is 0.6 nm / s. The thickness is 300 nm; the conductive polymer layer polyaniline is spin-coated at a speed of 2000 rpm with a thickness of 80 nm; then annealed at 200 ° C for 1 hour in a nitrogen atmosphere; then the silicone tube is fixed on the surface of the device with glue and sealed into a A water storage tank; finally, a gold wire electrode is inserted into the water storage tank as a grid; an organic electrochemical transistor marine microbial sensor is obtained.

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Abstract

The invention relates to a marine microorganism adhesion sensor based on an organic electrochemical transistor and a preparation method of the sensor. The sensor consists of a substrate (1), a drain electrode (2), a source electrode (3), a conductive polymer layer (4) with a microorganism detection function, a water storage pond (5) and a gate electrode (6), wherein the drain electrode (2) and the source electrode (3) are located above the substrate (1); the conductive polymer layer (4) is connected with the drain electrode (2) and the source electrode (3); the water storage pond (5) is located above the conductive polymer layer (4); the gate electrode (6) is located in the water storage pond (5), suspended above the conductive polymer layer (4) and connected with the conductive polymer layer (4) through electrolyte. The preparation method comprises the following preparation steps: washing and drying the substrate; preparing the source electrode and the drain electrode on the surface of the substrate; preparing a conductive polymer detection layer between the source electrode and the drain electrode; mounting the water storage pond on the surface of the conductive polymer layer. The marine microorganism adhesion sensor is simple in structure, simple in preparation method and easy to manufacture.

Description

technical field [0001] The invention belongs to the field of biosensors, in particular to a marine microorganism attachment sensor based on an organic electrochemical transistor and a preparation method. [0002] Background technique [0003] In the marine environment, microorganisms such as bacteria and diatoms usually quickly attach to the surface of the material and form a microbial film, causing microbial fouling of marine engineering equipment, greatly affecting the stability and safety of the equipment, and even losing work ability. At the same time, the microbial film is also the "soil" for the subsequent attachment of large marine organisms such as barnacles, oysters, and mussels. If the evolution process of the early microbial film is prevented, the subsequent large-scale attachment will be prevented. Therefore, it is particularly important to quickly detect the adhesion of marine microorganisms in order to provide a basis for taking certain protective measures i...

Claims

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Application Information

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IPC IPC(8): G01N27/26
Inventor 林仕伟廖建军张蕊蕊杨岳
Owner HAINAN UNIVERSITY
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