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Fluorescent oxygen nano sensor with cellular mitochondrion targeting, and preparation method of nano sensor

A nano-sensor and mitochondrial technology, applied in nanostructure manufacturing, nanotechnology, nanotechnology, etc., to achieve the effects of good mitochondrial targeting, high oxygen sensitivity, and good biocompatibility

Inactive Publication Date: 2014-02-12
BEIJING JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the specific fluorescence detection of dissolved oxygen in mitochondria is still a challenging task.

Method used

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  • Fluorescent oxygen nano sensor with cellular mitochondrion targeting, and preparation method of nano sensor
  • Fluorescent oxygen nano sensor with cellular mitochondrion targeting, and preparation method of nano sensor
  • Fluorescent oxygen nano sensor with cellular mitochondrion targeting, and preparation method of nano sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Dissolve PtTFPP, PS and DTS in tetrahydrofuran at a mass ratio of 2:48:50, and make their total concentration in the solution 0.2%. Then, using a micro-adjusting syringe, take 500 μL of the above solution, and quickly inject it into deionized water with a pH value of 9 (adjust the pH value with ammonia water) under the condition of ultrasonic vibration, and the resulting suspension is subjected to ultrasonic vibration for 2 hours, and then Dialyzed in secondary water for 24 hours, the nanoparticles used for measuring the oxygen content outside the cells were obtained.

[0024] (2) A mixed solution of the same concentration of PtTFPP, PS and DTS was quickly injected into deionized water with a pH value of 9 and a PLL concentration of 0.02mg / ml under the condition of ultrasonic vibration, and the resulting suspension was ultrasonically oscillated for 2 hours. , followed by dialysis in secondary water for 24 hours (to remove the organic solvent and excess PLL), the PLL...

Embodiment 2

[0036] Repeat Example 1, the difference is only platinum (II) MESO-tetrakis (pentafluorobenzene) porphine molecule, polystyrene and dodecyltrimethoxysilane mass ratio is according to 1:49:50, more The concentration of polylysine in deionized water is 0.01 mg / ml.

Embodiment 3

[0038] Repeat Example 1, the only difference is that the mass ratio of platinum (II) MESO-tetrakis(pentafluorobenzene) porphine molecule, polystyrene and dodecyltrimethoxysilane is 3:47:50.

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Abstract

The invention discloses a fluorescent oxygen nano sensor with cellular mitochondrion targeting. The nano sensor has the fluorescent service life sensitive to dissolved oxygen quantity, and can specifically target a mitochondrion in a cell. After the cell phagocytizes the oxygen nano sensor, variations of the dissolved oxygen quantity in a cytoplasm and the mitochondrion can be detected by a variation of the fluorescent service life of the nano sensor. The fluorescent oxygen nano sensor with the cellular mitochondrion targeting has the following characteristics that (1) a PLL (Phase Locked Loop) housing layer allows the sensor to have good organism compatibility; encapsulation allows a probe to have external interference resistance; (2) the mitochondrion targeting is good; (3) the oxygen sensitivity of the fluorescent service life is high; (4) the dissolved oxygen quantity in different positions in the cell can be detected.

Description

technical field [0001] The invention belongs to the technical field of fluorescent detection of biological dissolved oxygen, and in particular relates to a fluorescent oxygen nanometer sensor with cell mitochondria targeting and a preparation method thereof. Background technique [0002] Mitochondria are important organelles in cells and play an important role in maintaining the homeostasis of physiological functions. Mitochondrial dysfunction is directly related to a series of pathological diseases, such as obesity, diabetes, tumors, and cardiovascular diseases. Therefore, the detection of mitochondrial function in cells is of great significance. As we all know, mitochondria are the main place for intracellular aerobic respiration to generate energy, and most of the oxygen in cells is utilized by mitochondria. If the dissolved oxygen level in mitochondria can be monitored in real time, it can indirectly reflect the activity of mitochondria and its metabolic function. [0...

Claims

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Application Information

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IPC IPC(8): G01N21/64B82B3/00
Inventor 彭洪尚王小卉
Owner BEIJING JIAOTONG UNIV
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