Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation method of paclitaxel silicon plastid microcapsule

A siliceous body and paclitaxel technology, applied in the field of biomedical materials, can solve the problems of uniform particle size, wide particle size distribution range of siliceous body, affecting practical application, etc., and achieves good stability, uniform and controllable particle size, and size. More controllable effects

Inactive Publication Date: 2014-02-19
SUN YAT SEN UNIV
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a drug carrier, siliceous bodies have solved the various deficiencies of liposomes well, but there is a problem at the same time, that is, the particle size distribution of siliceous bodies is wide, and the particle size cannot be made uniform by conventional preparation methods. affect their future practical application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of paclitaxel silicon plastid microcapsule
  • Preparation method of paclitaxel silicon plastid microcapsule
  • Preparation method of paclitaxel silicon plastid microcapsule

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Prepare 0.025M Na respectively 2 CO 3 、 Ca(NO 3 ) 2 100mL each solution Ca(NO 3 ) 2 Add 400mg polystyrene sodium sulfonate (PSS) to the solution, after it is completely dissolved, quickly add Na 2 CO 3 Solution, stirred at 600 rpm for 15s, left to stand for 20min, centrifuged and washed three times to obtain calcium carbonate particles with a particle size of 5 microns, the laser confocal photo is shown in figure 2 .

[0035] (2) Precisely weigh 10 mg of organic-inorganic composite lipid 1 and dissolve it in 2 mL of chloroform, remove the chloroform by rotary evaporation, and form a transparent lipid film at the bottom of the eggplant-shaped bottle. 4mg / mL uniform particle size and positively charged CaCO 3 Add 5 mL of the microsphere solution into the lipid film, incubate in a water bath at 45°C for 30 min, and then sonicate in a water bath for 5 min. After standing at room temperature for 12 hours, add 0.2M EDTA solution to react for a period of ...

Embodiment 2

[0038] (1) Same as Step 1 of Example 1;

[0039] (2) Same as step 2 of embodiment 1;

[0040] (3) Same as Step 3 of Example 1, the siliceous microcapsules loaded with doxorubicin hydrochloride are released in the phosphate buffer solution of pH=7.4 and pH=5.0, and the drug release rate is detected, see Figure 5 . This experimental group can demonstrate that doxorubicin hydrochloride encapsulated in siliceous microcapsules can play a slow-release effect on the drug, and has a good slow-release effect under neutral or acidic conditions.

Embodiment 3

[0042] (1) Same as Step 1 of Example 1;

[0043] (2) Same as Step 2 of Example 1, except that the lipid used is organic-inorganic composite lipid 2.

[0044] (3) Same as Step 3 of Example 1, the encapsulation efficiency of doxorubicin hydrochloride was 84.4%. It shows that the siliceous microcapsules prepared with organic-inorganic composite lipid 2 have the ability to encapsulate hydrophilic drugs, and the encapsulation efficiency is very high.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of a paclitaxel silicon plastid microcapsule. The method comprises the following steps: taking calcium carbonate microspheres as a template; according to sol-gel and self-assembly methods, mounting organic-inorganic compound lipids to the surfaces of the colloid microspheres; at the same time, loading a lipid-soluble anticancer drug on a lipid bilayer; according to a chemical method, removing the template calcium carbonate microspheres to obtain the silicon plastid microcapsule with uniform and controllable grain size, stable properties and monodisperse performance; jointly hatching the silicon plastid microcapsule and a water-soluble anticancer drug, so as to embed the water-soluble anticancer drug into the silicon plastid microcapsule. The organic-inorganic compound lipids for preparing the microcapsule are non-toxic, and have excellent biocompatibility. The preparation method is simple in process, mild in operation conditions, high in repeatability, good in slow-release effect, and meanwhile suitable for embedding and controllably releasing the lipid-soluble anticancer drug and the water-soluble anticancer drug, and has an excellent application prospect.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to an anticancer drug siliceous microcapsule and a preparation method thereof. Background technique [0002] Malignant tumors are one of the diseases that seriously endanger human health, and chemotherapy is currently an indispensable treatment. However, the current chemotherapeutic drugs not only kill tumor cells but also kill normal cells after entering the human body in a free form, causing serious toxic and side effects. Clinically, the side effects of anticancer drugs are mainly manifested in: inhibition of bone marrow hematopoietic function, reduction of platelets and leukocytes, and gastrointestinal reactions. Some drugs have obvious toxicity to the heart and liver. These toxic effects increase the suffering of patients during chemotherapy and largely limit the clinical application of anticancer drugs. Liposomes have been paid more and more attention as carriers of diagn...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/50A61K31/337A61K47/24A61P35/00
Inventor 曹众张春阳蒋庆
Owner SUN YAT SEN UNIV