Portable high-resolution optical fiber CARS microscopic excitation source device and implementation method

Abstract

The invention discloses a portable high-resolution optical fiber CARS microscopic excitation source device and an implementation method. The device sequentially comprises a femtosecond optical fiber laser light source (1), a beam splitter (2), an electronically-controlled phase delayer (3), an analyzer (4), a photonic crystal fiber (5), a multimode fiber (6), a single mode fiber (7), an electronically-controlled delay line (8), a beam combiner (9), an output port (11) and a control module (10) according to the sequence from input to output. The portable high-resolution optical fiber CARS microscopic excitation source device has the advantages that the optical fiber structure is adopted, environment anti-interference performance is high, and the device is portable and has the ability to be quickly connected with a commercialized microscope to enable a CARS imaging function to be achieved; chirp processing can be carried out on pulsed light by means of the combined optical fiber structure, the stable and reliable spectral compression is achieved, and the spectral resolution is improved.

Description

technical field [0001] The invention belongs to the field of nonlinear optical imaging, and in particular relates to a portable high-resolution optical fiber excitation source for CARS microscopic imaging, which can be used for detection of material components in the fields of biology and chemistry. Background technique [0002] Coherent Anti-Stokes Raman Scattering (CARS) uses four-wave mixing to detect molecular vibrations. When the frequency difference between pump light and Stokes light is equal to the vibration frequency of the molecule to be detected, the natural vibration of the molecule is resonantly enhanced. , when the probe light interacts with it, a strong anti-Stokes scattering signal will be generated. The CARS signal is generated in the coherent process, and the signal has strong directionality and is easy to collect. The CARS signal is anti-Stokes light, which is blue-shifted relative to the excitation light, and it is easy to filter out the interference of ...

AI Technical Summary

Problems solved by technology

At present, CARS microscopy and spectroscopy systems mainly use two Ti:Sapphire lasers with feedback control or two optical parametric oscillators pumped by the same pump source as the excitation source. It is difficult to adjust the synchronization of pump light and Stokes light. Wavelength tuning uses a mechanical device, the tuning speed is slow, and the repeatability is poor, which makes the whole system huge and complicated, requiring full-time technicians to maintain and adjust the system, which seriously hinders the wide application of CARS

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