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Preparation method for effective part of valerianajatamansi epoxy iridoid ester

A technology for fragrant epoxy iridoid ester and effective part, which is applied in the field of preparation of the effective part of spider fragrant epoxy iridoid ester, can solve the problems affecting the purity of the effective part of the iridoid ester, blockage of network pores, Affect the purification effect and other problems, and achieve the effect of less extraction solvent, small dosage and high extraction efficiency

Active Publication Date: 2014-02-26
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the iridoid esters are oily substances with strong fat solubility, and their solubility in water is very small. Therefore, when the sample is applied, the volume of the aqueous solution will be relatively large. If the dissolution is not complete, the iridoid esters will precipitate out and cause macropores. The blockage of the net-like pores of the adsorption resin affects the purification effect; ethanol has a high polarity, and even when ethanol with a high quality fraction (such as 95% ethanol) is eluted, a large amount of non-iridoid esters with high polarity will be eluted. The impurities are eluted to the effective part, which in turn affects the purity of the effective part of the iridoid ester
[0010] In terms of the application of effective parts of iridoid esters of Valeriana genus Patraceae, literature reports mostly focus on sedation, hypnosis, antidepressant, etc., and there are few reports on its application in cancer treatment

Method used

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  • Preparation method for effective part of valerianajatamansi epoxy iridoid ester

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The preparation method of the effective part of the spider aromatic iridoid ester comprises the following steps:

[0028] A. Extraction: powder the medicinal materials of spider incense, and pass through a 40-mesh sieve for later use. Take 7kg of the coarse powder of spider incense, add an appropriate amount of extraction solvent to moisten it, and then pack it into a column, add an appropriate amount of 95% ethanol for cold soaking for 24 hours, then extract by percolation, and collect the percolation liquid. The total solvent volume for extraction is equivalent to 15 times the mass of medicinal materials (a total of 105L of 95% ethanol is used).

[0029] B. Extraction: Concentrate the percolation liquid obtained in step A to near dryness under reduced pressure to obtain 527.4 g of thick extract, add water to suspend to 3 L, and use 3 L of dichloromethane, 3 L of ethyl acetate, and 3 L of n-butanol respectively Extraction, each extraction solvent was extracted three t...

Embodiment 2

[0033] A. Extraction: Take 7kg of the medicinal material of spider incense, add 8 times the amount (56L) of 95% ethanol, and extract under reflux at 85°C. The extraction time is 2 hours each time, and the two extracts are combined. A total of 112L of 95% ethanol was used.

[0034] B. Extraction: Concentrate the extract obtained in step A to near dryness under reduced pressure to obtain 586.4 g of thick extract, add water to suspend to 3 L, extract with 3 L of dichloromethane, 3 L of ethyl acetate, and 3 L of n-butanol respectively , each extraction solvent was extracted three times, and the extracts were combined to obtain the dichloromethane extract, ethyl acetate extract and n-butanol extract respectively, which were separated by thin layer chromatography (TLC) and developed by Epstahl reagent. , the blue spots of the spider incense epoxy iridoid esters are mainly concentrated in the dichloromethane extract, and the dichloromethane extract is decompressed and the solvent is ...

Embodiment 3

[0038] A. Extraction: powder the medicinal materials of spider incense, and pass through a 40-mesh sieve for later use. Take 7kg of the coarse powder of spider incense, add extraction solvent to moisten it, pack it into a column, add an appropriate amount of 75% ethanol for cold soaking for 24 hours, and then extract by percolation. ) to collect the percolate.

[0039] B. Extraction: Concentrate the percolation liquid obtained in step A to nearly dryness under reduced pressure to obtain 613.5 g of thick extract, add water to suspend to 3 L, and use 3 L of dichloromethane, 3 L of ethyl acetate, and 3 L of n-butanol respectively Extraction, each extraction solvent was extracted three times, and the extracts were combined to obtain the dichloromethane extract, ethyl acetate extract, and n-butanol extract respectively, which were separated by thin layer chromatography (TLC) and purified by Epstahl reagent. For color development, the blue spots of Epoxyiridoid Epoxyides are mainly...

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Abstract

A provided preparation method for the effective part of valerianajatamansi epoxy iridoid ester comprises: A, using an extraction solvent to performing extraction on medicinal material valerianajatamansi to obtain the extract; B, concentrating the extract, adding water, mixing, extracting with dichloromethane to obtain the extract liquor, extracting the extract liquor to obtain epoxy iridoid ester crude extract; and C, performing positive column chromatography on the epoxy iridoid ester crude product, eluting with an eluant, collecting the eluate and drying to obtain the effective part of valerianajatamansi epoxy iridoid ester, wherein the eluant is an eluant composed of petroleum ether and ethyl acetate with a volume ratio of 15-2:1. The valerianajatamansi epoxy iridoid ester effective part prepared by employing the method is strong in biological activity, simple in operation and low in production cost, has the advantages of being stable in stable, controllable and the like, and is applicable to preparation of cancer treating medicines.

Description

(1) Technical field [0001] The invention relates to a preparation method of the effective part of epoxyiridoid ester of spider fragrance. (2) Background technology [0002] There are more than 200 species of Valeriana L. plants in the family Valerianaceae, which are widely distributed in the northern temperate regions of America, Europe and Asia. There are 17 species and 2 varieties in my country, with the most species distributed in the west and southwest. As a medicinal plant, Valeriana officinalis Linn (Valeriana officinalis Linn), Valeriana officinalis Linn (Valeriana officinalis Linn), Valeriana officinalis Linn (Valeriana officinalis Linn .var.latifolia Miq), etc. This genus of plants has a long history of medicine in Europe, and can be used to treat mild and moderate insomnia in Europe, America and other countries. [0003] Spider incense is a plant of the genus Valeriana in the Patriaceae family. It is widely distributed in my country and has a history of many yea...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P35/00
Inventor 杨波谷满仓程汝滨王一奇陈佳丽张如松
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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