Method for improving degradation effect of ochratoxin A (OTA) in solution by using electron beam irradiation

A technology of ochratoxin and electron beam irradiation, which is applied in the direction of protection devices against harmful chemicals, etc., and can solve problems such as complicated operation, restriction, and incomplete degradation

Active Publication Date: 2014-02-26
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are many ways to degrade OTA, including physical methods, chemical methods, biological methods, etc., but most of these methods

Method used

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  • Method for improving degradation effect of ochratoxin A (OTA) in solution by using electron beam irradiation
  • Method for improving degradation effect of ochratoxin A (OTA) in solution by using electron beam irradiation
  • Method for improving degradation effect of ochratoxin A (OTA) in solution by using electron beam irradiation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1, the effect of different rays on OTA degradation effect

[0026] At room temperature,

[0027] The OTA standard stock solution was diluted with water to 100ng / ml, respectively, using 0-10kGy electron beam and 60 Co-γ rays were irradiated.

[0028] Result analysis:

[0029] Such as figure 2 As shown, the x-axis represents the irradiation intensity, the y-axis represents the ratio of the initial concentration of the OTA solution to the concentration after degradation, ■ represents the electron beam, ● represents 60 Co-gamma rays.

[0030] The degradation effect of ochratoxin A in the solution is different with different types of radiation. When the dose is low, the radiation degradation effect of electron beam on OTA is slightly higher than that of 60 Co-gamma rays. At 1kGy, the degradation rates were 70.98% and 61.92%, respectively. With the increase of dose, the increase of degradation rate tends to be stable. At 7.5 kGy, OTA was almost completely...

Embodiment 2

[0031] Embodiment 2, the influence of different solvents on OTA degradation effect

[0032] At room temperature,

[0033] The OTA standard stock solution was diluted to 100 ng / mL with water, acetonitrile, 60% methanol and hydrogen peroxide solution; the electron beam with the intensity of 0-25 kGy was used for irradiation treatment.

[0034] Result analysis:

[0035] Such as image 3 As shown, the x-axis represents the irradiation intensity, the y-axis represents the ratio of the initial concentration of the OTA solution to the concentration after degradation, ■ indicates that the solvent of the OTA solution is water, ● indicates that the solvent of the OTA solution is acetonitrile, and ▲ indicates the solvent of the OTA solution is 60% methanol, ▼ indicates that the solvent of the OTA solution is hydrogen peroxide.

[0036] After electron beam irradiation, OTA was effectively degraded, and the degradation rate of OTA in these four solvents increased with the increase of do...

Embodiment 3

[0037] Embodiment 3, initial concentration is to the degradation effect test of OTA in the aqueous solution

[0038] At room temperature,

[0039] Three OTA aqueous solutions with different concentrations of 50ng / mL, 100ng / mL and 200ng / mL were prepared and irradiated by electron beams with an intensity of 0-25kGy.

[0040] Result analysis

[0041] Such as Figure 4 As shown, the x-axis represents the irradiation intensity, and the y-axis represents the ratio of the initial concentration of the OTA aqueous solution to the concentration after degradation. OTA aqueous solution with a concentration of 200ng / mL.

[0042]The initial concentration of OTA aqueous solution has certain influence on the degradation of OTA. The degradation rate of OTA in the three OTA aqueous solutions was 50ng / ml>100ng / ml>200ng / ml in turn, that is, low initial concentration was beneficial to the degradation of OTA. At 5kGy, the degradation rates of three different concentrations of OTA aqueous solut...

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Abstract

The invention discloses a method for improving the degradation effect of ochratoxin A (OTA) in a solution by using electron beam irradiation. The method is characterized in that the OTA in the aqueous solution is subjected to irradiating treatment by adopting electron beams generated by an electron accelerator, and.OH with extremely strong oxidizability, hydrated electrons (a formula shown in the specification) with extremely strong reducibility and H. radicals are produced due to the radiolysis of water after treatment, wherein.OH free radicals can be subjected to addition reaction with benzene rings and unsaturated bonds of OTA, and the Cl element of OTA is easily attacked by the hydrated electrons, so that OTA is dechlorinated; after OTA is dechlorinated, the H. free radicals are rapidly added to groups, so that OTA is degraded. The process conditions for improving the degradation effect are as follows: the pH value of the OTA aqueous solution is adjusted to be 7-10 during irradiation, the initial concentration of the solution is not higher than 200ng/mL, and the degradation rate of OTA is over 98% when the adopted radiation dose is 1-12kGy. The method has the advantages that the operation is simple and convenient, the effect is remarkable, and OTA in the OTA aqueous solution can be effectively degraded.

Description

technical field [0001] The invention relates to a method for improving the degradation effect of ochratoxin A in a solution by using electron beam irradiation, and belongs to the technical field of degrading mycotoxins in food and environmental protection. Background technique [0002] OTA (ochratoxin A) is a toxic secondary metabolite produced by Penicillium and Aspergillus species. OTA is relatively stable, not easy to degrade, and widely exists in nature. Crops and feed become its main pollution objects, which seriously threaten the health of humans and animals. A large number of data show that OTA has certain carcinogenicity, teratogenicity and mutagenicity, as well as strong nephrotoxicity, liver toxicity, neurotoxicity and immunotoxicity, and its toxicity is second only to aflatoxin. In 1993, the International Agency for Research on Cancer listed OTA as a Class 2B carcinogen. In 2011, China revised GB2701-2011 "Limits of Mycotoxins in Food", which clearly stipulates ...

Claims

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Application Information

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IPC IPC(8): A62D3/15A62D101/20
Inventor 李淑荣彭春红周林燕魏明张乐李亚茹曹珍
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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