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Method for treating intestinal residue in production process of crude product heparin sodium

A technology of production process and treatment method, which is applied in the field of intestinal residue treatment in the production process of crude heparin sodium, to achieve obvious effects, improve recovery rate, and increase benefits

Active Publication Date: 2014-02-26
南通天龙畜产品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the production of heparin sodium, the salt solution method will inevitably lead to incomplete dissociation of heparin in the heparin-protein complex. At high temperature, the protein will wrap the undissociated heparin, denature and form intestinal residue, resulting in a decrease in the recovery rate of crude heparin sodium. Phenomenon

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Dissolution: Take 400g of intestinal residue from the workshop, add 800mL of water to dissolve, stir and heat to 60°C;

[0024] (2) Enzymolysis: Add NaOH solution to the reaction solution to adjust the pH to 9.5, then add 40 g of 2709 alkaline protease to the reaction solution, after 2.5 hours of reaction, add HCl to adjust the pH to neutral, raise the temperature to 80°C, and keep it warm for 10 minutes Centrate

[0025] (3) Adsorption: add water to adjust the salinity of the centrifuge to 2.0° at 60°C, add 3.2g of heparin-specific adsorption resin, and absorb for 8 hours;

[0026] (4) Desorption: collect the adsorbed resin, desorb with 23 ° brine for 3 times, and collect the desorption liquid;

[0027] (5) Precipitation: add ethanol with a volume fraction of 90% to the desorption solution for precipitation, let it stand for 24 hours, and collect the lower layer of precipitation;

[0028] (6) Dehydration and drying: Dehydrate the obtained precipitate with 95% eth...

Embodiment 2

[0030] (7) Dissolution: Take 800g of intestinal residue from the workshop, add 1600mL of water to dissolve, stir and heat to 60°C;

[0031] (8) Enzymolysis: Add NaOH solution to the reaction solution to adjust the pH to 9.5, then add 80 g of 2709 alkaline protease to the reaction solution, after 2.5 hours of reaction, add HCl to adjust the pH to neutral, heat up to 80°C, and keep warm for 10 minutes Centrate

[0032] (9) Adsorption: add water to adjust the salinity of the centrifuge to 2.0° at 60°C, add 6.4g of heparin-specific adsorption resin, and absorb for 8 hours;

[0033] (10) Desorption: collect the adsorbed resin, desorb with 23 ° brine for 3 times, and collect the desorption liquid;

[0034] (11) Precipitation: add ethanol with a volume fraction of 90% to the desorption solution for precipitation, let it stand for 24 hours, and collect the lower layer of precipitation;

[0035] (12) Dehydration and drying: Dehydrate the obtained precipitate with 95% ethanol, let it ...

Embodiment 3

[0037] (13) Dissolution: Take 1200g of intestinal residue from the workshop, add 2400mL of water to dissolve, stir and heat to 60°C;

[0038] (14) Enzymolysis: Add NaOH solution to the reaction solution to adjust the pH to 9.5, then add 120 g of 2709 alkaline protease to the reaction solution, after 2.5 hours of reaction, add HCl to adjust the pH to neutral, heat up to 80°C, and keep warm for 10 minutes Centrate

[0039] (15) Adsorption: add water to adjust the salinity of the centrifuge to 2.0° at 60°C, add 9.6g of heparin-specific adsorption resin, and absorb for 8 hours;

[0040] (16) Desorption: collect the adsorbed resin, desorb with 23 ° brine for 3 times, and collect the desorption liquid;

[0041] (17) Precipitation: add ethanol with a volume fraction of 90% to the desorption solution for precipitation, let it stand for 24 hours, and collect the lower layer of precipitation;

[0042] (18) Dehydration and drying: the obtained precipitate was dehydrated with 95% ethano...

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Abstract

The invention relates to the technical field of heparin sodium production, and specifically relates to a method for treating intestinal residue in the production process of crude product heparin sodium. The method for treating the intestinal residue in the production process of crude product heparin sodium comprises the steps of dissolving, enzymolysis, adsorption, desorption, precipitation, and dehydration and drying. The method provided by the invention has the advantages of mature process, simple operation and obvious effect, therefore, the recovery rate of crude product heparin sodium is effectively increased and the economic benefits of a producer are improved; in the adsorption process, an adsorption solution formed after adsorption is directly spray-dried to obtain intestinal protein peptide powder which can be used for preparing feeds for young animals; in this way, the residual heparin in the intestinal residue is recovered, and the protein in the intestinal residue can also be effectively utilized; consequently, environmental pollution is avoided and the benefits are also increased.

Description

Technical field: [0001] The present invention involves the field of heparin sodium production technology, and specifically involves the treatment method of intestinal residue in the production process of a rough heparin sodium. Background technique: [0002] Heparin exists in the hearts, liver, spleen, lungs, thymus, muscles, blood vessels, etc., but mainly derived from the tissue of large cells in animals, such as liver, lung, and end intestine.Core Protein (CP) forms heparin proteoglycan (HEP-PG).Heparin is a polysaccharide chain composed of a polysacchal chain composed of repeated two sugar chains connected by 1 → 4 keys in a polysaccharide chain.Do not wait for 10-30 dietary units, 2-O-sulfuric acid-α-L an Eydinhenylic acid and 6-O-sulfate-α-D glycosamine are the main sugar of them.They formed that the duplicate unit of trigly sulfate is the so -called "rule zone" of heparin and is the main part of the heparin structure. [0003] The large -scale preparation research of hepa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/10
Inventor 刘亚男许春龙孙学超张玉根鲍程刘洪星韩国栋
Owner 南通天龙畜产品有限公司
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