Lipidosome encapsulating epigallocatechin gallate and preparation method thereof

A technology for epigallocatechin and gallate, which is applied in the field of liposomes and preparations for encapsulating epigallocatechin gallate, and can solve the problems of prone to oxidation, polymerization, condensation, low bioavailability, Low bioavailability and other problems, to achieve the effect of easy popularization and application, low equipment requirements, and simple preparation process

Active Publication Date: 2014-03-05
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because EGCG has strong oxidation resistance, it is very easy to undergo oxidation, polymerization, condensation and other reactions under conditions such as humidity, sunlight, and high temperature, and its bioavailability is not high in an unprotected state. Problem, it is necessary to embed EGCG and prepare EGCG liposomes to improve its bioavailability and solve the problems of its low bioavailability and poor oil solubility

Method used

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  • Lipidosome encapsulating epigallocatechin gallate and preparation method thereof
  • Lipidosome encapsulating epigallocatechin gallate and preparation method thereof
  • Lipidosome encapsulating epigallocatechin gallate and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Dissolve 90mg of soybean lecithin and 30mg of cholesterol in a mixed solution containing 6ml of chloroform and 9ml of ether to obtain liquid A, dissolve 15mg of EGCG in 3ml of phosphate buffer solution to obtain liquid B, and mix the two solutions of liquid A and liquid B , first magnetically stirred for 1.5h, and then ultrasonicated for 5min under the condition of a water bath sonicator with a frequency of 100Hz, so that the mixed solution became a clear single phase; in a water bath at 40°C, under a vacuum pressure of 0.09 Mpa, the mixed solution was rotary evaporated until Form a layer of lipid film; add 300 μl Tween-80 and 30 ml phosphate buffer solution to the lipid film to dissolve the lipid film to obtain solution C; leave solution C at room temperature for 2 hours, and then pass through 0.8 μm Filter paper, 0.45 μm filter membrane, and store the filtrate in a refrigerator at 4°C to obtain liposomes encapsulating EGCG.

[0026] Phosphate buffer configuration:

...

Embodiment 2

[0033] Dissolve 80mg of soybean lecithin and 40mg of cholesterol in a mixed solution containing 6ml of chloroform and 9ml of ether to obtain liquid A, dissolve 18mg of EGCG in 3ml of phosphate buffer solution to obtain liquid B, and mix the two solutions of liquid A and liquid B , first magnetically stirred for 1.5h, and then ultrasonicated for 5min under the condition of a water bath sonicator with a frequency of 100Hz, so that the mixed solution became a clear single phase; then the mixed solution was rotary evaporated in a water bath at 40°C under a vacuum pressure of 0.09 Mpa Until a layer of lipid film is formed; add 300 μl Tween-80 and 30 ml phosphate buffer solution to the lipid film to dissolve the lipid film to obtain solution C; leave solution C at room temperature for 2 hours, and then pass through 0.8 μm filter paper, 0.45 μm filter membrane, and store the filtrate in a refrigerator at 4°C to obtain EGCG-encapsulated liposomes.

[0034] The configuration of the pho...

Embodiment 3

[0036] Dissolve 100mg of soybean lecithin and 20mg of cholesterol in a mixed solution containing 6ml of chloroform and 9ml of ether to obtain liquid A, dissolve 13mg of EGCG in 3ml of phosphate buffer solution to obtain liquid B, and mix the two solutions of liquid A and liquid B , first magnetically stirred for 1.5h, and then ultrasonicated for 5min under the condition of a water bath sonicator with a frequency of 100Hz, so that the mixed solution became a clear single phase; in a water bath at 40°C, under a vacuum pressure of 0.09 Mpa, the mixed solution was rotary evaporated until Form a layer of lipid film; add 300 μl Tween-80 and 30 ml phosphate buffer solution to the lipid film to dissolve the lipid film to obtain solution C; leave solution C at room temperature for 2 hours, and then pass through 0.8 μm Filter paper, 0.45 μm filter membrane, and store the filtrate in a refrigerator at 4°C to obtain liposomes encapsulating EGCG.

[0037] The configuration of the phosphate...

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PUM

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Abstract

The invention discloses a lipidosome encapsulating epigallocatechin gallate and a preparation method process. The lipidosome consists of the following components: soya bean lecithin, cholesterol, epigallocatechin gallate, buffer liquor and tween-80. The preparation method disclosed by the invention is simple in process and the EGCG lipidosome is prepared by a reverse evaporation method, and ultrasonic treatment is carried out on the lipidosome by a water bath ultrasonic instrument till the system is stable. The method can remarkably improve the stability of the EGCG lipidosome, and the grain size of the lipidosome can be effectively controlled and the encapsulation efficiency can be effectively improved by adjusting the ratio of the lipid components and the buffer liquor through the technical steps such as magnetic stirring, ultrasonic treatment and rotary evaporation. The EGCG lipidosome disclosed by the invention develops the application range of lipidosome, and the lipidosome is used as a carrier to encapsulate EGCG, so that the lipidosome is simple in preparation process, lower in demand on equipment, convenient to popularize and apply and can be industrially produced.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a liposome encapsulating epigallocatechin gallate and a preparation method thereof. Background technique [0002] Liposome is an artificial membrane. The hydrophilic head of phospholipid molecule is inserted into the water, and the hydrophobic tail of liposome extends to the air. After stirring, a spherical liposome with bilayer lipid molecules is formed, with a diameter of 25-1000nm. Liposomes can be used for genetically modified or prepared drugs, using the characteristics of liposomes that can fuse with cell membranes to deliver drugs into cells. [0003] Epigallocatechin gallate (hereinafter referred to as EGCG) is the most effective antioxidant polyphenol in green tea, which has anti-oxidation, anti-cancer, anti-mutation and other activities. The antioxidant activity is at least 100 times that of vitamin C and 25 times that of vitamin E. It can protect cells and DNA...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K31/353A23L1/29A23P1/00A61P39/06A61P35/00A23L33/00
Inventor 骆晓波关荣发沈海涛赵进胡颖叶兴乾
Owner CHINA JILIANG UNIV
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