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Drop diterpenoid type compound as well as preparation method and application thereof

A compound, the technology of norditerpene, applied in the field of norditerpenoids, can solve the problems of serious residue, pollution, biological and human health hazards, etc., and achieve the effect of inhibiting bacteria and tumor cells

Active Publication Date: 2015-04-01
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Since the 20th century, the extensive use of chemically synthesized pesticides has brought serious pollution to water bodies and soils. More than 2 / 3 of the pesticides have directly penetrated into the environment, and the residues are very serious, causing direct and potential harm to both organisms and human health.

Method used

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  • Drop diterpenoid type compound as well as preparation method and application thereof
  • Drop diterpenoid type compound as well as preparation method and application thereof
  • Drop diterpenoid type compound as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The norditerpenoids derived from seaweed endophytic fungi are represented by formula (I).

[0026]

Embodiment 2

[0028] The preparation method of the alkaloid compound shown in formula (I):

[0029] Take the fungus Aspergillus wentii (Aspergillus wentii) NA3 strain that grew well on the plate and inoculate it in JDB liquid medium, put 300mL medium in each 1L Erlenmeyer flask, 30 bottles in total, ferment statically at room temperature for 30 days, filter, and collect mycelia body. The JDB liquid medium consists of 500 milliliters of Jerusalem artichoke tuber juice per liter, 10 grams of glucose, 2 grams of sodium nitrate, 20 micromoles of suberoylanilide hydroxamic acid (SAHA), and 500 milliliters of aged sea water.

[0030] The mycelium was dried and pulverized, extracted three times with chloroform-methanol at a volume ratio of 1:1, extracted with ethyl acetate, and concentrated under reduced pressure to obtain 5.72 g of crude extract. The crude extract was subjected to 200-300 mesh silica gel column chromatography, and petroleum ether-ethyl acetate was used at a volume ratio of 100:0...

Embodiment 3

[0036] The difference from Example 2 is that

[0037] Take the fungus Aspergillus wentii (Aspergillus wentii) NA3 strain that grew well on the plate and inoculate it in JDB liquid medium, put 300mL medium in each 1L Erlenmeyer flask, 100 bottles in total, ferment statically at room temperature for 20 days, filter, and collect mycelia body. The JDB liquid medium consists of 500 milliliters of Jerusalem artichoke tuber juice per liter, 10 grams of glucose, 2 grams of sodium nitrate, 10 micromoles of suberoylanilide hydroxamic acid (SAHA), and 500 milliliters of aged seawater.

[0038]The mycelium was dried and pulverized, extracted three times with chloroform-methanol at a volume ratio of 1:1, then extracted with ethyl acetate, and concentrated under reduced pressure to obtain 18.00 g of crude extract. The crude extract was subjected to 200-300 mesh silica gel column chromatography, and petroleum ether-ethyl acetate was used at a volume ratio of 100:0, 50:1, 20:1, 10:1, 5:1, 2:...

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Abstract

The invention relates to the field of insecticides, bacteriostats and tumor cell inhibitors, and in particular relates to a drop diterpenoid type compound from seaweed endophytes as well as a preparation method and application of the drop diterpenoid type compound. The drop diterpenoid type compound has a structural formula shown by a formula (I). The preparation method of the compound comprises the steps of inoculating Aspergillus wentii NA3 to a fungus liquid culture medium for fermentation culture, and purifying a fermentation product to obtain the drop diterpenoid type compound shown by the formula (I). An insecticidal activity test shows that the median lethal concentration of the drop diterpenoid type compound obtained by the preparation method disclosed by the invention is 1.81 micrograms per milliliter, and meanwhile, the compound has activities of inhibiting bacteria and tumor cells.

Description

technical field [0001] The invention relates to the fields of insecticides, bacteriostatic agents and tumor cell inhibitors, in particular to a norditerpene compound derived from seaweed endophytic fungi and its preparation and application. Background technique [0002] Since the 20th century, the extensive use of chemically synthesized pesticides has brought serious pollution to water bodies and soils. More than 2 / 3 of the pesticides have directly penetrated into the environment, and the residues are very serious, causing direct and potential harm to organisms and human health. . Compared with chemically synthesized pesticides, biological pesticides have the advantages of good environmental compatibility and less resistance. Their development and application are of great significance to human health, environmental protection and sustainable development of agriculture. [0003] Bacterial diseases remain an important type of disease in humans and other organisms. Due to the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C49/747A01N35/06A01P7/04A01P1/00A61K31/122A61P31/04A61P35/00C12P7/26C12R1/71
CPCA01N35/06C07C49/747C07C2603/26C12P7/26
Inventor 季乃云苗凤萍
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI