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A kind of preparation method of high-purity phosphatidylcholine

A phosphatidylcholine, high-purity technology, applied in the field of phospholipids, can solve the problems of cost reduction, high processing difficulty, high cost, etc., and achieves the effects of simple overall process, easy regeneration and application, and short production cycle

Active Publication Date: 2016-04-27
WENGYUAN GUANGYE QINGYI FOOD TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many steps in the solvent method, and there are many types of solvents used. Acetone has to be used in large quantities in industrial production, which poses a great safety hazard.
And the column chromatography method reported at present mainly contains alumina method (U.S.Pat.No4235793,4528139,5453523), macroporous resin chromatography (CN102633832) etc., aluminum oxide and macroporous resin are to other phospholipids in the above-mentioned method The adsorption capacity is very strong, it is easy to form dead adsorption, and the filler regeneration is difficult. In addition, there are other separation and purification methods such as the expensive supercritical fluid method and the chemical denaturation method that is more difficult to treat after adding reaction reagents, etc.
In terms of production on an industrial scale, none of the above methods can better simplify steps, simplify operations and reduce costs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] At room temperature, soak 500g of SephadexLH-20 dry powder in 50% ethanol by volume for 28 hours, and keep stirring to ensure that the gel swells, wash off the residual ethanol with anhydrous salt water, and then dry the gel with volume Ethanol with a percentage content of 97% was used to replace and dissolve the slurry. After the swollen gel was degassed, it was placed in the column at one time according to the column packing requirements. Take 100g of sunflower seed alcohol-soluble crude phospholipids (with a PC content of 24%) as raw material, prepare a 20mg / mL ethanol solution with 95% ethanol by volume, and dissolve the alcohol-soluble phospholipids in ethanol at a constant flow rate of 3mL / min. The solution was added to a Sephadex LH-20 chromatographic column of Sephadex, followed by eluting with 95% ethanol as the eluent at a flow rate of 3mL / min, collecting the eluate, and detecting its composition by TLC. , the parts containing phosphatidylcholine were combined...

Embodiment 2

[0026] At room temperature, soak 500g of SephadexLH-20 dry powder in ethanol with a volume percentage of 50% for 24 hours, and keep stirring to ensure the swelling of the gel, wash with anhydrous ethanol to remove the residual ethanol and filter to dry. Then the gel was replaced with 100% ethanol and slurried. After the swollen gel was degassed, it was put into the column at one time according to the packing requirements. Take 105 g of soybean alcohol-soluble crude phospholipids (with a PC content of 20%) as a raw material, and prepare an ethanol solution of 22 mg / mL alcohol-soluble phospholipids with a volume percentage of 95% ethanol, and dissolve the alcohol-soluble phospholipids at a constant flow rate of 3 mL / min. The ethanol solution was added to the Sephadex LH-20 chromatographic column of Sephadex, followed by eluting with 95% ethanol as the eluent at a flow rate of 4mL / min, collecting the eluate, and detecting it by TLC. Composition, the parts containing phosphatidylc...

Embodiment 3

[0028] At room temperature, soak 500g of SephadexLH-20 dry powder in ethanol with a volume percentage of 50% for 27 hours, and keep stirring to ensure the swelling of the gel, wash with anhydrous ethanol to remove residual ethanol and filter to dry. Then the gel was replaced with 98% ethanol by volume and slurried. After the swollen gel was degassed, it was put into the column at one time according to the column packing requirements. Take 115g of soybean-derived alcohol-soluble crude phospholipids (with a PC content of 23%) as raw materials, prepare a 24 mg / mL ethanol solution of alcohol-soluble phospholipids with 97% ethanol by volume, and dissolve the alcohol-soluble crude phospholipids at a constant flow rate of 4 mL / min. The ethanol solution of phospholipids was added to the Sephadex LH-20 chromatography column of Sephadex, followed by eluting with 97% ethanol as the eluent at a flow rate of 5mL / min, collecting the eluate, and detecting it by TLC Its composition, the part ...

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Abstract

The invention discloses a preparation method of high-purity phosphatidylcholine. The preparation method comprises the following steps: preparing an alcohol-soluble raw phospholipid ethanol solution by taking low-alcohol alcohol-soluble raw phospholipid as a raw material; loading the alcohol-soluble raw phospholipid ethanol solution into a dextrangel SephadexLH-20 chromatographic column; after the alcohol-soluble raw phospholipid ethanol solution is eluted by using high-concentration ethanol; collecting an eluent; removing solvents in the eluent by evaporating, so that high-purity phosphatidylcholine is obtained. Dextrangel SephadexLH-2 used in the invention has less possibility of forming dead adsorption and is easily regenerated and used indiscriminately, and eluted solvents are single and non-toxic; solvents adopted in the preparation method are less in type, and high in indiscriminate use degree in production; the preparation method disclosed by the invention is simple in whole process and short in production cycle, the purity of obtained products is over 85%, and the industrialized production is easily realized.

Description

technical field [0001] The invention belongs to the technical field of phospholipids, in particular to a method for preparing high-purity phosphatidylcholine. Background technique [0002] Phospholipids, also known as phospholipids and phospholipids, are lipids containing phosphoric acid and belong to complex lipids. Phospholipids are the main components of biological membranes, which are divided into two categories: glycerophospholipids and sphingomyelins, which are composed of glycerol and sphingosine respectively. Phospholipids are amphiphilic molecules with a hydrophilic nitrogen or phosphorus tail at one end and a long hydrophobic (oleophilic) hydrocarbon chain at the other end. Phospholipids can be divided into glycerolphospholipids and sphingolipids according to their skeletons. They are all polar fats. Polar lipids consist of a polar part (called the polar head) and a nonpolar part (called the nonpolar tail). Among them, glycerophospholipids can be divided into p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07F9/10
Inventor 杜阳吉梁丽敏刘春凤
Owner WENGYUAN GUANGYE QINGYI FOOD TECH
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