Method for preparing oligopeptide through mixed enzymolysis and fermentation of fish meal processing waste liquid and vegetable proteins

A plant protein and mixed enzymatic hydrolysis technology, which is applied in food processing, animal feed, animal feed, etc., can solve the problems of unfavorable large-scale production, reduced oligopeptide content, and cumbersome preparation steps, etc., and achieves remarkable enzymatic hydrolysis effect and nutritional Balanced and fully enzymatic effect

Inactive Publication Date: 2014-03-12
YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Patent CN200710168967.0 discloses a method for preparing functional feed protein enteric membrane protein powder. This method adopts a segmented liquid enzymatic hydrolysis method with multiple enzymes. This method pursues high-efficiency enzymatic hydrolysis, resulting in a relatively h

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0019] Example 1

[0020] 400 kg of crushed cotton meal, 400 kg of rapeseed meal and 200 kg of fresh white wine grains, mix with 1000 kg of concentrated silver carp and fish meal waste liquid, adjust pH to 5.5-7.0, add compound enzyme preparation, stir evenly, enzymatically In 2 hours, the enzymatic hydrolysis temperature is controlled within the range of 30℃-40℃. Enzyme preparations include neutral protease 2000u / g, papain 3000u / g, glucanase 1000u / g, and cellulase 1000u / g. After the enzymolysis, 5 kg of Bacillus subtilis, 4 kg of feed yeast, and 5 kg of Lactobacillus plantarum were added, mixed and fermented for 64 hours. Aeration is taken every 6-8 hours for 40s-80s, the fermentation temperature is controlled below 45℃ by turning the material and cooling measures, and the closed anaerobic fermentation is carried out after 48 hours of fermentation. After fermentation is completed, it is dried, crushed and packaged.

[0021] Silver carp is generally the first choice fish for fis...

Example Embodiment

[0022] Example 2

[0023] 500 kg of crushed cotton meal, 500 kg of rapeseed meal, and 100 kg of fresh white distiller’s grains, mix with 600 kg of concentrated fish meal waste liquid, adjust pH to 5.5-7.0, add compound enzyme preparation, stir evenly, enzymolysis for 2 hours, The temperature of enzymolysis is controlled within the range of 30℃-40℃. Enzyme preparations include neutral protease 1000u / g, alkaline protease 1000u / g, papain 2000u / g, glucanase 4000u / g, and cellulase 1000u / g. After the enzymolysis, 3 kg of Bacillus subtilis, 2 kg of feed yeast, and 3 kg of Lactobacillus plantarum were added, mixed and fermented for 56 hours. Aeration is taken every 6-8 hours for 40s-80s, the fermentation temperature is controlled below 45℃ by turning the material and cooling measures, and the closed anaerobic fermentation is carried out after 48 hours of fermentation. After the fermentation is completed, it is directly sealed and vacuum packaged or dried, crushed and packaged.

Example Embodiment

[0024] Example 3

[0025] 1000 kg of crushed cotton meal, 1000 kg of rapeseed meal, and 500 kg of fresh white distiller’s grains, mixed with 900 kg of concentrated fish meal waste liquid, adjusted to pH 5.5-7.0, added compound enzyme preparation, stirred evenly, enzymatic hydrolysis for 2.5 hours , The temperature of enzymolysis is controlled within the range of 30℃-40℃. Enzyme preparations include neutral protease 1000u / g, papain 2000u / g, pectinase 1000u / g, glucanase 4000u / g, and cellulase 1000u / g. After the enzymolysis, 6 kg of Bacillus subtilis, 5 kg of feed yeast, and 8 kg of Lactobacillus plantarum were added, mixed and fermented for 48 hours. Aeration is taken every 6-8 hours for 40s-80s, the fermentation temperature is controlled below 45°C by turning the material and cooling measures, and the closed anaerobic fermentation is carried out after 30 hours of fermentation. After the fermentation is completed, it is directly sealed and vacuum packaged.

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Abstract

The invention belongs to the technical field of microbial enzymatic and fermented feeds, and provides a method for preparing oligopeptide through the mixed enzymolysis and fermentation of fish meal processing waste liquid and vegetable proteins. According to the method, an enzymolysis method and a fermentation method are adopted, so that proteins can be fully degraded so as to obtain low molecular oligopeptide with relatively high content, and a delicious amino acid, oligopeptide, nucleotides and natural organic acids also can be produced, so that the palatability is obviously increased. In addition, according to the method provided by the invention, cottonseed meal and rapeseed meal are adopted for replacing part of soybean meal, so that the cost can be effectively saved, and thus the economic benefits are improved.

Description

Technical field [0001] The invention belongs to the technical field of microbial enzymatic hydrolysis and fermentation of feed, and relates to a method for preparing oligopeptides by mixed enzymatic hydrolysis and fermentation of freshwater fish meal processing waste liquid and plant protein. Background technique [0002] With the rapid development of the intensification of aquaculture, the demand for fishmeal has increased rapidly. However, the continuous deterioration of the environment and overfishing have led to a decline in global fishmeal production and high prices. High-quality feed proteins such as fishmeal have also become restrictions on animal husbandry and The bottleneck of the development of aquaculture industry. Fish meal processing technology is mostly wet. The process is characterized in that the raw materials are crushed-cooked-squeezed-dried to make fish meal. In the fish meal processing process, every 1 ton of fish meal produced can produce 3 to 3.5 tons of sq...

Claims

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Application Information

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IPC IPC(8): A23K1/00A23K1/14A23K1/06A23K1/165A23K10/38
CPCY02P60/87
Inventor 袁科平毛涛曹喜秀韦扬帆
Owner YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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